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Abstract:

Two of the most potent cytokines regulating anterior pituitary cell function are leukemia inhibitory factor and interleukin-6 (IL-6), which belong to the cytokine receptor family using the common gp130 signal transducer. We studied the actions of two other members of this family, IL-11 and ciliary neurotropic factor (CNTF), on folliculostellate (FS) cells (TtT/GF cell line) and lactosomatotropic cells (GH3 cell line). The messenger RNA (mRNA) for the α-chain specific for the IL-11 receptor (1.7 kb) and CNTF receptor (2 kb) are expressed on both cell types. In addition, we detected CNTF receptor mRNA in normal rat anterior pituitary cells. IL-11 (1.25-5 nM) dose dependently stimulated the proliferation of FS cells. CNTF, at doses from 0.4-2 nM, also significantly stimulated the growth of these cells. In addition, both cytokines significantly stimulated proliferation of lactosomatotropic GH3 cells, and CNTF stimulated hormone production (GH and PRL) at 24 h by these cells. At 16-72 h, IL-11 stimulates the secretion of the angiogenic factor vascular endothelial growth factor by FS cells. In addition, both GH3 and FS cells express CNTF mRNA. These data suggest that IL-11 and CNTF may act as growth and regulatory factors in anterior pituitary cells.

Registro:

Documento: Artículo
Título:The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines
Autor:Castro, C.P.; Nagashima, A.C.; Pereda, M.P.; Goldberg, V.; Chervin, A.; Largen, P.; Renner, U.; Stalla, G.K.; Arzt, E.
Filiación:Laboratorio de Fisiología y Biología Molecular, Department de Biología, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Department of Endocrinology, Max Planck Institute of Psychiatry, 80804 Munich, Germany
Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, 1427 Buenos Aires, Argentina
Hospital Santa Lucía, 1232 Buenos Aires, Argentina
Laboratorio de Fisiología y Biología Molecular, Department de Biología, Pabellon II, 1428 Buenos Aires, Argentina
Argentine National Research Council, Argentina
Palabras clave:ciliary neurotrophic factor; ciliary neurotrophic factor receptor; cytokine; glycoprotein gp 130; interleukin 11; interleukin 11 receptor; leukemia inhibitory factor; vasculotropin; adenohypophysis; alpha chain; angiogenesis; animal cell; article; cell proliferation; controlled study; dose response; hormone synthesis; hypophysis cell; nonhuman; priority journal; rat; regulatory mechanism; signal transduction; stellate cell
Año:2000
Volumen:141
Número:5
Página de inicio:1746
Página de fin:1753
DOI: http://dx.doi.org/10.1210/endo.141.5.7442
Título revista:Endocrinology
Título revista abreviado:Endocrinology
ISSN:00137227
CODEN:ENDOA
CAS:vasculotropin, 127464-60-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v141_n5_p1746_Castro

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Citas:

---------- APA ----------
Castro, C.P., Nagashima, A.C., Pereda, M.P., Goldberg, V., Chervin, A., Largen, P., Renner, U.,..., Arzt, E. (2000) . The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines. Endocrinology, 141(5), 1746-1753.
http://dx.doi.org/10.1210/endo.141.5.7442
---------- CHICAGO ----------
Castro, C.P., Nagashima, A.C., Pereda, M.P., Goldberg, V., Chervin, A., Largen, P., et al. "The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines" . Endocrinology 141, no. 5 (2000) : 1746-1753.
http://dx.doi.org/10.1210/endo.141.5.7442
---------- MLA ----------
Castro, C.P., Nagashima, A.C., Pereda, M.P., Goldberg, V., Chervin, A., Largen, P., et al. "The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines" . Endocrinology, vol. 141, no. 5, 2000, pp. 1746-1753.
http://dx.doi.org/10.1210/endo.141.5.7442
---------- VANCOUVER ----------
Castro, C.P., Nagashima, A.C., Pereda, M.P., Goldberg, V., Chervin, A., Largen, P., et al. The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines. Endocrinology. 2000;141(5):1746-1753.
http://dx.doi.org/10.1210/endo.141.5.7442