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Abstract:

Bacterial lipopolysaccharide (LPS) activates the immune system and induces increases in peripheral cytokines, which, in turn, affect the endocrine system. In particular, LPS-induced cytokines stimulate the hypothalamic-pituitary-adrenal axis to increase levels of antiinflammatory-acting glucocorticoids. In the present work, we show that LPS directly stimulates interleukin (IL)-6 release by mouse pituitary folliculostellate (FS) TtT/GF tumor cells and FS cells of mouse pituitary cell cultures. The stimulatory effect of LPS was strongly enhanced in the presence of serum, suggesting that LPS is only fully active as a complex with LPS-binding protein (LBP). Both TtT/GF cells and mouse pituitaries expressed CD14, which binds the LPS/LBP complex, and Toll-like receptor type 4, which induces LPS signals. LPS increased phospoinositol turnover in TtT/GF cells and induced phosphorylation of p38α mitogen-activated protein kinase and the inhibitor (IκB) of nuclear factor-κ B. Nuclear factor-κ B was activated by LPS in TtT/GF cells. Functional studies demonstrated that My4 (an antibody blocking the interaction between LPS/LBP and CD14), SB203580, (a specific inhibitor of p38α mitogen-activated protein kinase phosphorylation), dexamethasone, and the messenger RNA translation inhibitor cycloheximide all inhibited LPS-induced IL-6 production by TtT/GF cells and mouse pituitary FS cells. LPS-induced intrapituitary IL-6 may modulate the function of anterior pituitary cells during bacterial infection/inflammation.

Registro:

Documento: Artículo
Título:Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
Autor:Lohrer, P.; Gloddek, J.; Carbia Nagashima, A.; Korali, Z.; Hopfner, U.; Paez Pereda, M.; Arzt, E.; Stalla, G.K.; Renner, U.
Filiación:Max Planck Institute of Psychiatry, Department of Endocrinology, D-80804 Munich, Germany
Laboratorio Fisiologia y Biologia Molecular, FCEN, Universidad de Buenos Aires and CONICET, 1427 Buenos Aires, Argentina
Max Planck Institute of Psychiatry, Department of Endocrinology, Kraepelin Street 10, D-80804 Munich, Germany
Palabras clave:4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole; antibody; CD14 antigen; cycloheximide; dexamethasone; immunoglobulin enhancer binding protein; interleukin 6; lipopolysaccharide; lipopolysaccharide binding protein; mitogen activated protein kinase; toll like receptor 4; animal cell; antigen expression; article; cell line; cell stimulation; complex formation; controlled study; cytokine production; cytokine release; hypophysis cell; male; mouse; nonhuman; priority journal; protein metabolism; protein phosphorylation; signal transduction
Año:2000
Volumen:141
Número:12
Página de inicio:4457
Página de fin:4465
DOI: http://dx.doi.org/10.1210/endo.141.12.7811
Título revista:Endocrinology
Título revista abreviado:Endocrinology
ISSN:00137227
CODEN:ENDOA
CAS:4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole, 152121-47-6; cycloheximide, 642-81-9, 66-81-9; dexamethasone, 50-02-2; lipopolysaccharide binding protein, 203946-66-1; mitogen activated protein kinase, 142243-02-5; toll like receptor 4, 203811-83-0
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00137227_v141_n12_p4457_Lohrer.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v141_n12_p4457_Lohrer

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Citas:

---------- APA ----------
Lohrer, P., Gloddek, J., Carbia Nagashima, A., Korali, Z., Hopfner, U., Paez Pereda, M., Arzt, E.,..., Renner, U. (2000) . Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway. Endocrinology, 141(12), 4457-4465.
http://dx.doi.org/10.1210/endo.141.12.7811
---------- CHICAGO ----------
Lohrer, P., Gloddek, J., Carbia Nagashima, A., Korali, Z., Hopfner, U., Paez Pereda, M., et al. "Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway" . Endocrinology 141, no. 12 (2000) : 4457-4465.
http://dx.doi.org/10.1210/endo.141.12.7811
---------- MLA ----------
Lohrer, P., Gloddek, J., Carbia Nagashima, A., Korali, Z., Hopfner, U., Paez Pereda, M., et al. "Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway" . Endocrinology, vol. 141, no. 12, 2000, pp. 4457-4465.
http://dx.doi.org/10.1210/endo.141.12.7811
---------- VANCOUVER ----------
Lohrer, P., Gloddek, J., Carbia Nagashima, A., Korali, Z., Hopfner, U., Paez Pereda, M., et al. Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway. Endocrinology. 2000;141(12):4457-4465.
http://dx.doi.org/10.1210/endo.141.12.7811