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Cells derived from an experimental luteinized ovarian tumor are more sensitive to GnRH endocrine action than control luteal cells. In an attempt to understand the possible causes of the differential sensibility to GnRH action, we examined the number and affinity of GnRH receptors and the second messenger response to GnRH stimulation in both tissues. For GnRH receptor studies membranes were obtained from 4-to 6-week-old ovarian tumors (luteoma) and ovaries from prepubertal rats treated with 25 IU PMSG and 25 IU hCG (SPO) and were incubated with [125I]Buserelin. The number of GnRH receptors were increased in luteoma compared with that in SPO ovaries; dissociation constants were similar in both tissues. GnRH stimulation of second messenger release was assessed in cells obtained from luteoma and SPO ovaries by collagenase treatment. Buserelin (100 ng/ml) induced a significant 35% calcium increase in SPO cells, as determined by the fura-2 method; in luteoma cells no response was observed after buserelin stimulation, although a calcium transient was induced by thapsigargin (0.5 μM), an inhibitor of Ca2+-adenosine triphosphatase associated with the endoplasmic reticulum. The effect of buserelin on inositol phosphates was evaluated after incubation of luteoma and SPO cells with [3H]myoinositol for 48 h. Buserelin induced a 400% increase in inositol trisphosphate in SPO cells. Again, luteoma cells did not respond to buserelin stimulation, although NaF (10 mM), an activator of G proteins coupled to phospholipase C, induced an 800% increase in inositol trisphosphate. Although the number of GnRH receptors is augmented in luteoma cells, justifying an increased endocrine response, neither inositol phosphates nor intracellular calcium were released by a GnRH analog, indicating the uncoupling of GnRH receptors from phospholipase C. These data provide evidence that the transformation of the ovary into a luteoma implies the acquisition of novel characteristics in the GnRH receptor second messenger-generating system.


Documento: Artículo
Título:Alterations in intracellular messengers mobilized by gonadotropin-releasing hormone in an experimental ovarian tumor
Autor:Chamson-Reig, A.; Pignataro, O.P.; Libertun, C.; Lux-Lantos, V.A.R.
Filiación:Instituto de Biología y Medicina Experimental, CONICET, 1428 Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:adenosine triphosphatase (calcium); buserelin; calcium; chorionic gonadotropin; collagenase; enzyme inhibitor; fluoride sodium; fura 2; gonadorelin; gonadorelin receptor; guanine nucleotide binding protein; inositol trisphosphate; phospholipase C; seric gonadotropin; thapsigargin; animal cell; animal experiment; animal model; article; calcium cell level; calcium mobilization; cell stimulation; controlled study; female; hormonal regulation; luteal cell; luteoma; nonhuman; ovary tumor; priority journal; rat; receptor affinity; receptor binding; second messenger
Página de inicio:3573
Página de fin:3580
Título revista:Endocrinology
Título revista abreviado:Endocrinology
CAS:adenosine triphosphatase (calcium); buserelin, 57982-77-1; calcium, 7440-70-2, 14092-94-5; chorionic gonadotropin, 9002-61-3; collagenase, 37288-86-1, 39433-96-0; fura 2, 96314-98-6; gonadorelin, 33515-09-2, 9034-40-6; inositol trisphosphate, 27121-73-9; phospholipase C, 9001-86-9; seric gonadotropin, 9002-70-4; thapsigargin, 67526-95-8


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---------- APA ----------
Chamson-Reig, A., Pignataro, O.P., Libertun, C. & Lux-Lantos, V.A.R. (1999) . Alterations in intracellular messengers mobilized by gonadotropin-releasing hormone in an experimental ovarian tumor. Endocrinology, 140(8), 3573-3580.
---------- CHICAGO ----------
Chamson-Reig, A., Pignataro, O.P., Libertun, C., Lux-Lantos, V.A.R. "Alterations in intracellular messengers mobilized by gonadotropin-releasing hormone in an experimental ovarian tumor" . Endocrinology 140, no. 8 (1999) : 3573-3580.
---------- MLA ----------
Chamson-Reig, A., Pignataro, O.P., Libertun, C., Lux-Lantos, V.A.R. "Alterations in intracellular messengers mobilized by gonadotropin-releasing hormone in an experimental ovarian tumor" . Endocrinology, vol. 140, no. 8, 1999, pp. 3573-3580.
---------- VANCOUVER ----------
Chamson-Reig, A., Pignataro, O.P., Libertun, C., Lux-Lantos, V.A.R. Alterations in intracellular messengers mobilized by gonadotropin-releasing hormone in an experimental ovarian tumor. Endocrinology. 1999;140(8):3573-3580.