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Abstract:

Messenger RNA (mRNA) for enzymes involved in adrenal steroid biosynthesis are expressed in the brain, and the coded enzymes have been shown to be active. The expression of mRNA for the cytochrome P-450 enzyme aldosterone synthase, crucial for the final step in the synthesis of aldosterone and the synthesis of aldosterone was studied in several anatomic areas of the rat brain. Expression of the mRNA for the aldosterone synthase was demonstrated by RT-PCR/Southern blot in adrenal, aorta, hypothalamus, hippocampus, amygdala, cerebrum, and cerebellum. Incubation of brain minces from intact and adrenalectomized rats demonstrated the synthesis of corticosterone and aldosterone from endogenous precursors. Incubations of brain mince, with [1,23H]-deoxycorticosterone, followed by extraction and three different successive TLCs, demonstrated the presence of labeled aldosterone, corticosterone, and 18-hydroxy-deoxycorticosterone. Incubation, in the presence of 10 μM cortisol or metyrapone, inhibited the synthesis of aldosterone or both aldosterone and corticosterone, respectively. These studies indicate that the rat brain has the enzymatic machinery for the synthesis of adrenal corticosteroids and is capable of synthesizing aldosterone. Aldosterone synthesized in the brain might play a paracrine role in the regulation of blood pressure.

Registro:

Documento: Artículo
Título:Aldosterone biosynthesis in the rat brain
Autor:Gomez-Sanchez, C.E.; Zhou, M.Y.; Cozza, E.N.; Morita, H.; Foecking, M.F.; Gomez-Sanchez, E.P.
Filiación:Endocrinology Section, Harry S. Truman Mem. Vet. Hospital, University of Missouri-Columbia, Columbia, MO 65201, United States
Department of Internal Medicine, University of Missouri-Columbia, Columbia, MO 65201, United States
Dept. of Vet. Biomedical Sciences, University of Missouri-Columbia, Columbia, MO 65201, United States
Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Research Service, Harry S. Truman Mem. Vet. Hospital, Columbia, MO 65201, United States
Palabras clave:18 hydroxydeoxycorticosterone; aldosterone; corticosterone; messenger RNA; synthetase; amygdaloid nucleus; animal tissue; article; brain; cerebellum; controlled study; gene expression; hippocampus; hormone synthesis; hypothalamus; male; nonhuman; priority journal; rat; reverse transcription polymerase chain reaction; Southern blotting; steroidogenesis
Año:1997
Volumen:138
Número:8
Página de inicio:3369
Página de fin:3373
DOI: http://dx.doi.org/10.1210/endo.138.8.5326
Título revista:Endocrinology
Título revista abreviado:ENDOCRINOLOGY
ISSN:00137227
CODEN:ENDOA
CAS:18 hydroxydeoxycorticosterone, 379-68-0; aldosterone, 52-39-1, 6251-69-0; corticosterone, 50-22-6; synthetase, 9031-56-5, 9031-57-6
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00137227_v138_n8_p3369_GomezSanchez.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v138_n8_p3369_GomezSanchez

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Citas:

---------- APA ----------
Gomez-Sanchez, C.E., Zhou, M.Y., Cozza, E.N., Morita, H., Foecking, M.F. & Gomez-Sanchez, E.P. (1997) . Aldosterone biosynthesis in the rat brain. Endocrinology, 138(8), 3369-3373.
http://dx.doi.org/10.1210/endo.138.8.5326
---------- CHICAGO ----------
Gomez-Sanchez, C.E., Zhou, M.Y., Cozza, E.N., Morita, H., Foecking, M.F., Gomez-Sanchez, E.P. "Aldosterone biosynthesis in the rat brain" . Endocrinology 138, no. 8 (1997) : 3369-3373.
http://dx.doi.org/10.1210/endo.138.8.5326
---------- MLA ----------
Gomez-Sanchez, C.E., Zhou, M.Y., Cozza, E.N., Morita, H., Foecking, M.F., Gomez-Sanchez, E.P. "Aldosterone biosynthesis in the rat brain" . Endocrinology, vol. 138, no. 8, 1997, pp. 3369-3373.
http://dx.doi.org/10.1210/endo.138.8.5326
---------- VANCOUVER ----------
Gomez-Sanchez, C.E., Zhou, M.Y., Cozza, E.N., Morita, H., Foecking, M.F., Gomez-Sanchez, E.P. Aldosterone biosynthesis in the rat brain. ENDOCRINOLOGY. 1997;138(8):3369-3373.
http://dx.doi.org/10.1210/endo.138.8.5326