Saravia-Fernandez, F.; Faveeuw, C.; Blasquez-Bulant, C.; Tappaz, M.; Throsby, M.; Pelletier, G.; Vaudry, H.; Dardenne, M.; Homo-Delarche, F. "Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse" (1996) Endocrinology. 137(8):3497-3506
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Glutamic acid decarboxylase (GAD), among other potential autoantigens, is thought to play a crucial role in type I diabetes, particularly in a spontaneous model of the disease, the nonobese diabetic (NOD) mouse. In the pancreas, the presence of GAD and γ-aminobutyric acid (GABA), the decarboxylation product of GAD and a putative neurotransmitter in the islets of Langerhans, is well documented in the β-cells. This is particularly true in rats, in which another GABAergic structure exists near the islets, the neuronal bodies. In this study, first the GABA content was measured in isolated islets from NOD and C57BL/6 mice (controls), and a decrease was found in NOD females as their insulitis progressed. Second, for the first time in mice, confocal analysis of immunofluorescent-labeled pancreatic sections revealed near the islets neuronal structures in which GAD and neuropeptide Y were colocalized, as they are in the brain. These structures were always observed in the pancreata of both sexes of C57BL/6 mice at the various ages investigated. In NOD mice, however, these neuronal structures were only detected in young females (< 10 weeks old) and in males until an intermediate age. Moreover, patches oft cells surrounding GAD-containing fibers were seen in the vicinity of the islets with incipient periinsulitis.


Documento: Artículo
Título:Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse
Autor:Saravia-Fernandez, F.; Faveeuw, C.; Blasquez-Bulant, C.; Tappaz, M.; Throsby, M.; Pelletier, G.; Vaudry, H.; Dardenne, M.; Homo-Delarche, F.
Filiación:Ctr. Natl. Rech. Sci. U. Rech. A., Université de Paris V, Hôpital Necker, 75015 Paris, France
Inst. de Biol. y Med. Experimental, 1428 Buenos Aires, Argentina
INSERM U413, Université de Rouen, 76821 Mont Saint-Aignan, France
INSERM U171, 69230 Saint-Genis-Laval, France
Med. Res. Ctr. Grp. Molec. E., Ctr. Hosp. et Universitaire Laval, Qué. GIV4G2, Canada
CNRS URA 1461, Hôpital Necker, 161, rue de Sèvres, 75743 Paris Cedex 15, France
Palabras clave:4 aminobutyric acid; glutamate decarboxylase; neuropeptide Y; animal model; animal tissue; article; controlled study; diabetes mellitus; enzyme analysis; enzyme localization; immunohistochemistry; insulitis; mouse; nonhuman; pancreas; priority journal; T lymphocyte; tissue distribution
Página de inicio:3497
Página de fin:3506
Título revista:Endocrinology
Título revista abreviado:ENDOCRINOLOGY
CAS:4 aminobutyric acid, 28805-76-7, 56-12-2; glutamate decarboxylase, 9024-58-2; neuropeptide Y, 82785-45-3, 83589-17-7


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---------- APA ----------
Saravia-Fernandez, F., Faveeuw, C., Blasquez-Bulant, C., Tappaz, M., Throsby, M., Pelletier, G., Vaudry, H.,..., Homo-Delarche, F. (1996) . Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse. Endocrinology, 137(8), 3497-3506.
---------- CHICAGO ----------
Saravia-Fernandez, F., Faveeuw, C., Blasquez-Bulant, C., Tappaz, M., Throsby, M., Pelletier, G., et al. "Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse" . Endocrinology 137, no. 8 (1996) : 3497-3506.
---------- MLA ----------
Saravia-Fernandez, F., Faveeuw, C., Blasquez-Bulant, C., Tappaz, M., Throsby, M., Pelletier, G., et al. "Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse" . Endocrinology, vol. 137, no. 8, 1996, pp. 3497-3506.
---------- VANCOUVER ----------
Saravia-Fernandez, F., Faveeuw, C., Blasquez-Bulant, C., Tappaz, M., Throsby, M., Pelletier, G., et al. Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse. ENDOCRINOLOGY. 1996;137(8):3497-3506.