Artículo

Saravia-Fernandez, F.; Faveeuw, C.; Blasquez-Bulant, C.; Tappaz, M.; Throsby, M.; Pelletier, G.; Vaudry, H.; Dardenne, M.; Homo-Delarche, F. "Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse" (1996) Endocrinology. 137(8):3497-3506
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Glutamic acid decarboxylase (GAD), among other potential autoantigens, is thought to play a crucial role in type I diabetes, particularly in a spontaneous model of the disease, the nonobese diabetic (NOD) mouse. In the pancreas, the presence of GAD and γ-aminobutyric acid (GABA), the decarboxylation product of GAD and a putative neurotransmitter in the islets of Langerhans, is well documented in the β-cells. This is particularly true in rats, in which another GABAergic structure exists near the islets, the neuronal bodies. In this study, first the GABA content was measured in isolated islets from NOD and C57BL/6 mice (controls), and a decrease was found in NOD females as their insulitis progressed. Second, for the first time in mice, confocal analysis of immunofluorescent-labeled pancreatic sections revealed near the islets neuronal structures in which GAD and neuropeptide Y were colocalized, as they are in the brain. These structures were always observed in the pancreata of both sexes of C57BL/6 mice at the various ages investigated. In NOD mice, however, these neuronal structures were only detected in young females (< 10 weeks old) and in males until an intermediate age. Moreover, patches oft cells surrounding GAD-containing fibers were seen in the vicinity of the islets with incipient periinsulitis.

Registro:

Documento: Artículo
Título:Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse
Autor:Saravia-Fernandez, F.; Faveeuw, C.; Blasquez-Bulant, C.; Tappaz, M.; Throsby, M.; Pelletier, G.; Vaudry, H.; Dardenne, M.; Homo-Delarche, F.
Filiación:Ctr. Natl. Rech. Sci. U. Rech. A., Université de Paris V, Hôpital Necker, 75015 Paris, France
Inst. de Biol. y Med. Experimental, 1428 Buenos Aires, Argentina
INSERM U413, Université de Rouen, 76821 Mont Saint-Aignan, France
INSERM U171, 69230 Saint-Genis-Laval, France
Med. Res. Ctr. Grp. Molec. E., Ctr. Hosp. et Universitaire Laval, Qué. GIV4G2, Canada
CNRS URA 1461, Hôpital Necker, 161, rue de Sèvres, 75743 Paris Cedex 15, France
Palabras clave:4 aminobutyric acid; glutamate decarboxylase; neuropeptide Y; animal model; animal tissue; article; controlled study; diabetes mellitus; enzyme analysis; enzyme localization; immunohistochemistry; insulitis; mouse; nonhuman; pancreas; priority journal; T lymphocyte; tissue distribution
Año:1996
Volumen:137
Número:8
Página de inicio:3497
Página de fin:3506
DOI: http://dx.doi.org/10.1210/endo.137.8.8754779
Título revista:Endocrinology
Título revista abreviado:ENDOCRINOLOGY
ISSN:00137227
CODEN:ENDOA
CAS:4 aminobutyric acid, 28805-76-7, 56-12-2; glutamate decarboxylase, 9024-58-2; neuropeptide Y, 82785-45-3, 83589-17-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v137_n8_p3497_SaraviaFernandez

Referencias:

  • Martin, D.L., Rimvall, K., Regulation of gamma-aminobutyric acid synthesis in the brain (1993) J Neurochem, 60, pp. 395-407
  • Erdo, S.L., Peripheral GABAergic mechanisms (1985) Trends Pharmacol Sci, 6, pp. 205-208
  • Erdo, S., Wolff, J.R., Gamma-aminobutyric acid outside the mammalian brain (1990) J Neurochem, 54, pp. 363-372
  • Michalik, M., Erecinska, M., GABA in pancreatic islets: Metabolism and function (1992) Biochem Pharmacol, 44, pp. 1-9
  • Solimena, M., De Camilli, P., Spotlight on a neuronal enzyme (1993) Nature, 366, pp. 15-17
  • Kaufman, D.L., Tobin, A.J., Glutamate decarboxylases and autoimmunity in insulin-dependent diabetes (1993) Trends Pharmacol Sci, 14, pp. 107-109
  • Baekkeskov, S., Aanstoot, H.J., Christgau, S., Reetz, A., Solimena, M., Cascalho, M., Folli, F., De Camilli, P., Identification of the 64K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylase (1990) Nature, 347, pp. 151-156
  • Solimena, M., De Camilli, P., Autoimmunity to glutamic acid decarboxylase (GAD) in stiff-man syndrome and insulin-dependent diabetes mellitus (1991) Trends Neurosci, 14, pp. 452-457
  • Harrison, L.C., Islet cell antigens in insulin-dependent diabetes: Pandora's box revisited (1992) Immunol Today, 13, pp. 348-352
  • Karlsen, A.E., Michelsen, B.K., Petersen, J.S., Hagopian, W.A., Grubin, C.E., Boel, E., Madsen, O.D., Lernmark, A., Glutamic acid decarboxylase, an autoantigen in insulin-dependent diabetes mellitus (1992) Diabetes Nutr Metab, 5, pp. 97-103
  • De Aizpurua, H.J., Harrison, L.C., Glutamic acid decarboxylase in insulin-dependent diabetes mellitus (1992) Diabetes Metab Rev, 8, pp. 133-147
  • Atkinson, M.A., MacLaren, N.K., Islet cell autoantigens in insulin-dependent diabetes (1993) J Clin Invest, 92, pp. 1608-1616
  • Bach, J.F., Insulin-dependent diabetes mellitus as an autoimmune disease (1994) Endocr Rev, 15, pp. 516-542
  • Kaufman, D.L., Clare-Salzer, M., Tian, J., Forsthuber, T., Ting, G.S.P., Robinson, P., Atkinson, M.A., Lehmann, P.V., Spontaneous loss of T-cell tolerance to glutamic acid decarboxylase in murine insulin-dependent diabetes (1993) Nature, 366, pp. 69-72
  • Tisch, R., Yang, X.D., Singer, S.M., Liblau, R.S., Fugger, L., McDevitt, H.O., Immune response to glutamic acid decarboxylase correlates with insulitis in non-obese diabetic mice (1993) Nature, 366, pp. 72-75
  • Jansen, A., Homo-Delarche, F., Hooijkaas, H., Leenen, P.J., Dardenne, M., Drexhage, H.A., Immunohistochemical characterization of monocytes-macrophages and dendritic cells involved in the initiation of the insulitis and beta cell destruction in NOD mice (1994) Diabetes, 43, pp. 667-675
  • Kaufman, D.L., Erlander, M.G., Clare-Salzier, M., Atkinson, M.A., MacLaren, N.K., Tobin, A.J., Autoimmunity to two forms of glutamate decarboxylase in insulin-dependent diabetes mellitus (1992) J Clin Invest, 89, pp. 283-292
  • Petersen, J.S., Karlsen, A.E., Markholst, H., Worsaae, A., Dyrberg, T., Michelsen, B., Neonatal tolerization with glutamic acid decarboxylase but not with bovine serum albumin delays the onset of diabetes in NOD mice (1994) Diabetes, 43, pp. 1478-1484
  • Elliott, J.F., Qin, H.Y., Bhatti, S., Smith, D.K., Singh, R.K., Dillon, T., Lauzon, J., Singh, B., Immunization with the larger isoform of mouse glutamic acid decarboxylase (GAD(67)) prevents autoimmune diabetes in NOD mice (1994) Diabetes, 43, pp. 1494-1499
  • Pleau, J.M., Femandez-Saravia, F., Esling, A., Homo-Delarche, F., Dardenne, M., Prevention of autoimmune diabetes in NOD female mice by treatment with recombinant glutamic acid decarboxylase (GAD 65) (1995) Clin Immunol Immunopathol, 76, pp. 90-95
  • Okada, Y., Taniguchi, H., Shimada, C., High concentration of GABA and high glutamate decarboxylase activity in rat pancreatic islets and human insulinoma (1976) Science, 191, pp. 620-622
  • Drummond, R.J., Phillips, A.T., L-Glutamic acid decarboxylase in non-neural tissues of the mouse (1974) J Neurochem, 23, pp. 1207-1213
  • Garry, D.J., Garry, M.G., Sorenson, R.L., Ultrastructural immunocytochemical localization of L-glutamate decarboxylase and GABA in rat pancreatic zymogen granules (1988) Cell Tissue Res, 252, pp. 191-197
  • Garry, D.J., Appel, N.M., Garry, M.G., Sorenson, R.L., Cellular and subcellular immunological localization of L-glutamate decarboxylase in rat pancreatic islets (1988) J Histochem Cytochem, 36, pp. 573-580
  • Garry, D.J., Sorenson, R.L., Coulter, H.D., Ultrastructural localization of gamma-amino-butyric acid immunoreactivity in beta-cells of the rat pancreas (1987) Diabetologia, 30, pp. 115-119
  • Sorenson, R.L., Garry, D.G., Brelje, T.C., Structural and functional considerations of GABA in islets of Langerhans: Beta cells and nerves (1991) Diabetes, 40, pp. 1365-1374
  • Gilon, P., Campistron, G., Geffard, M., Remacle, C., Immunocytochemical localization of GABA in endocrine cells of the rat entero-pancreatic system (1988) Biol Cell, 62, pp. 265-273
  • Reetz, A., Solimena, M., Matteoli, M., Folli, F., Takei, K., De Camilli, P., GABA and pancreatic beta-cells: Colocalization of glutamic acid decarboxylase (GAD) and GABA with synaptic-like microvesicles suggests their role in GABA storage and secretion (1991) EMBO J, 10, pp. 1275-1284
  • Michalik, M., Nelson, J., Erecinska, M., GABA production in rat islets of Langerhans (1993) Diabetes, 42, pp. 1506-1513
  • Gerber III, J.C., Hare, T.A., GABA in peripheral tissues: Presence and actions in endocrine pancreatic function (1980) Brain Res Bull, 5, pp. 341-346
  • Vincent, S.R., Hokfelt, T., Wu, J.Y., Elde, R.P., Morgan, L.M., Kimmel, J.R., Immunohistochemical studies of the GABA system in the pancreas (1983) Neuroendocrinology, 36, pp. 197-204
  • Sakaue, M., Saito, N., Tanaka, C., Immunohistochemical localization of gamma-aminobutyric acid (GABA) in the rat pancreas (1987) Histochemistry, 86, pp. 365-369
  • Vives-Pi, M., Somoza, N., Vargas, F., Armengol, P., Sarri, Y., Wu, J.Y., Pujol-Borrell, R., Expression of glutamic acid decarboxylase (GAD) in the alpha, beta and delta cells of normal and diabetic pancreas: Implications for the pathogenesis of type I diabetes (1993) Clin Exp Immunol, 92, pp. 391-396
  • Velloso, L.A., Kampe, O., Eizirik, D.L., Hallberg, A., Andersson, A., Karlsson, F.A., Human autoantibodies react with glutamic acid decarboxylase antigen in human and rat but not in mouse pancreatic islets (1993) Diabetologia, 36, pp. 39-46
  • Kim, J., Richter, W., Aanstoot, H.J., Shi, Y., Fu, Q., Rajotte, R., Warnock, G., Baekkeskov, S., Differential expression of GAD 65 and GAD 67 in human, rat and mouse pancreatic islets (1993) Diabetes, 42, pp. 1799-1808
  • Li, L., Jiang, J., Hagopian, W.A., Karlsen, A.E., Skelly, M., Baskin, D.G., Lemmark, A., Differential detection of rat islet and brain glutamic acid decarboxylase (GAD) isoforms with sequence-specific antibodies (1995) J Histochem Cytochem, 43, pp. 53-59
  • Faulkner-Jones, B.E., Cram, D.S., Kun, J., Harrison, L.C., Localization and quantitation of expression of two glutamate decarboxylase genes in pancreatic beta-cells and other peripheral tissues of mouse and rat (1993) Endocrinology, 133, pp. 2962-2972
  • Miller, R.E., Pancreatic neuroendocrinology: Peripheral neural mechanisms in the regulation of the islets of Langerhans (1981) Endocr Rev, 2, pp. 471-494
  • Honjin, R., The innervation of the pancreas of the mouse, with special reference to the structure of the peripheral extension of the vegetative nervous system (1956) J Comp Neurol, 104, pp. 331-371
  • Godfrey, D.A., Matschinsky, F.M., Enzymes of the cholinergic system in islets of Langerhans (1975) J Histochem Cytochem, 23, pp. 645-651
  • Lundquist, I., Ericson, L.E., Adrenergic insulin release and adrenergic innervation of mouse pancreatic islets (1978) Cell Tissue Res, 193, pp. 73-85
  • Kamel, I., Mikhail, Y., Beshir, S., Study of the innervation of the pancreas of the rat (1979) Acta Anat, 104, pp. 237-241. , Basel
  • Luiten, P.G.M., Ter Horst, G.J., Buijs, R.M., Steffens, A.B., Autonomic innervation of the pancreas in diabetic and non-diabetic rats. A new view on intramural sympathetic structural organization (1986) J Auton Nerv Syst, 15, pp. 33-44
  • Sundler, F., Bottcher, G., Islet innervation, with special reference to neuropeptides (1991) The Endocrine Pancreas, pp. 29-52. , Samols E (ed) Raven Press, New York
  • Amrani, A., Chaouloff, F., Mormede, P., Dardenne, M., Homo-Delarche, F., Glucose, insulin and open-field responses to immobilization in nonobese diabetic (NOD) mice (1994) Physiol Behav, 56, pp. 241-246
  • Durant, S., Coulaud, J., Amrani, A., El Hasnaoui, A., Dardenne, M., Homo-Delarche, F., Effects of various environmental stress paradigms and adrenalectomy on the expression of autoimmune type 1 diabetes in the nonobese diabetic (NOD) mouse (1993) J Autoimmun, 6, pp. 735-751
  • Fitzpatrick, F., Lepault, F., Homo-Delarche, F., Bach, J.F., Dardenne, M., Influence of castration, alone or combined with thymectomy, on the development of diabetes in the nonobese diabetic mouse (1991) Endocrinology, 129, pp. 1382-1390
  • Cubells, J.F., Blanchard, J.S., Makman, M.H., The effects of in vivo inactivation of GABA-transaminase and glutamic acid decarboxylase on levels of GABA in the rat retina (1987) Brain Res, 419, pp. 208-215
  • Shah, B.H., Hausman, R.E., Effect of insulin on GABAergic development in the embryonic chick retina (1993) Dev Brain Res, 72, pp. 151-158
  • Tappaz, M.L., Brownstein, M.J., Palkovits, M., Distribution of glutamate decarboxylase in discrete brain nuclei (1976) Brain Res, 108, pp. 371-379
  • Oertel, W.H., De Schmechel, Tappaz, M.L., Kopin, I.J., Production of a specific antiserum to rat brain glutamic acid decarboxylase by injection of an antigen-antibody complex (1981) Neuroscience, 6, pp. 2689-2700
  • Oertel, W.H., Schmechel, D.E., Mugnaini, E., Tappaz, M.L., Kopin, I.J., Immunocytochemical localization of glutamate decarboxylase in rat cerebellum with a new antiserum (1981) Neuroscience, 6, pp. 2715-2735
  • Heckroth, J.A., Development of glutamic acid decarboxylase-immunoreactive elements in the cerebellar cortex of normal and lurcher mutant mice (1992) J Comp Neurol, 315, pp. 85-97
  • Gilon, P., Tappaz, M., Remacle, C., Localization of GAD-like immunoreactivity in the pancreas and stomach of the rat and mouse (1991) Histochemistry, 96, pp. 353-365
  • Apud, J.A., Tappaz, M.L., Celotti, F., Negri-Cesi, P., Masotto, C., Racagni, G., Biochemical and immunochemical studies on the GABAergic system in the rat fallopian tube and ovary (1984) J Neurochem, 43, pp. 120-125
  • Tonon, M.C., Bosler, O., Stoeckel, M.E., Pelletier, G., Tappaz, M., Vaudry, H., Co-localization of tyrosine hydroxylase, GABA and neuropeptide T within axon terminals innervating the intermediate lobe of the frog Rana ridibunda (1992) J Comp Neurol, 319, pp. 599-605
  • Ledbetter, J.A., Herzenberg, L.A., Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens (1979) Immunol Rev, 47, pp. 63-80
  • Adjeroud, S., Tonon, M.C., Lamacz, M., Leneveu, E., Stoeckel, M.E., Tappaz, M.L., Cazin, L., Vaudry, H., GABA-ergic control of alpha-melanocyte-stimulating hormone (alpha-MSH) release by frog neurointermediate lobe in vitro (1986) Brain Res Bull, 17, pp. 717-723
  • Pelletier, G., Guy, J., Allen, Y.S., Polack, J.M., Electron microscope immunocytochemical localization of neuropeptide Y (NPY) in the rat brain (1984) Neuropeptides, 4, pp. 319-324
  • Danger, J.M., Guy, J., Benyamina, M., Jegou, S., Leboulenger, L.F., Coté, J., Tonon, M.C., Vaudry, H., Localization and identification of neuropeptide Y (NPY)-like immunoreactivity in the frog brain (1985) Peptides, 6, pp. 1225-1236
  • Gilon, P., Remade, C., High-affinity GABA uptake in a subpopulation of somatostatin cells in rat pancreas (1989) J Histochem Cytochem, 37, pp. 1133-1139
  • Okada, Y., Tanoguchi, H., Baba, S., High concentration of GABA in the pancreatic islets with special emphasis on beta-cells (1982) Problems in GABA Research from Brain to Bacteria, pp. 379-386. , Okada Y, Roberts E (eds) Excerpta Medica, Amsterdam
  • Gylfe, E., Hellman, B., Role of glucose as a regulator and precursor of amino acids in the pancreatic beta cells (1974) Endocrinology, 94, pp. 1150-1156
  • Briel, G., Gylfe, E., Hellman, B., Newhoff, V., Microdetermination of free amino acids in pancreatic islets isolated from obese-hyperglycemic mice (1972) Acta Physiol Scand, 84, pp. 247-253
  • Sundler, F., Moghimzadeh, E., Hakanson, R., Ekelund, M., Emson, P., Nerve fibers in the gut and pancreas of the rat displaying neuropeptide-Y immunoreactivity. Intrinsic and extrinsic origin (1983) Cell Tissue Res, 230, pp. 487-493
  • Lundberg, J.M., Terenius, L., Hökfelt, T., Tatemoto, K., Comparative immunohistochemical and biochemical analysis of pancreatic polypeptide-like peptides with special reference to presence of neuropeptide Y in central and peripheral neurons (1984) J Neurosci, 4, pp. 2376-2386
  • Carlei, F., Allen, J.M., Bishop, A.E., Bloom, S.R., Polak, J.M., Occurrence, distribution and nature of neuropeptide Y in the rat pancreas (1985) Experientia, 41, pp. 1554-1557
  • Dunning, B.E., Ahren, B., Böttcher, G., Sundler, F., Taborsky, G.J., The presence and actions of NPY in the canine endocrine pancreas (1987) Regul Pept, 18, pp. 253-265
  • De Giorgio, R., Sternini, C., Anderson, K., Brecha, N.C., Go, V.L.W., Tissue distribution and innervation pattern of peptide immunoreactivities in the rat pancreas (1992) Pediatrics, 13, pp. 91-98
  • De Giorgio, R., Sternini, C., Brecha, N.C., Widdison, A.L., Karangia, N.D., Reber, H.A., Go, V.L.W., Patterns of innervation of vasoactive intestinal polypeptide, neuropeptide Y, and gastrin-releasing peptide immunoreactive nerves in the feline pancreas (1992) Pancreas, 7, pp. 376-384
  • Alpert, S., Hanahan, D., Teitelman, G., Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons (1988) Cell, 53, pp. 295-308
  • Pettersson, M., Ahren, B., Lundquist, I., Böttcher, G., Sundler, F., Neuropeptide Y: Intrapancreatic neuronal localization and effects on insulin secretion in the mouse (1987) Cell Tissue Res, 248, pp. 43-48
  • Lee, Y., Shiosaka, S., Glemson, P.C., Powell, J.F., Smith, A.D., Tohyama, M., Neuropeptide Y-like immunoreactive structures in the rat stomach with special reference to the noradrenaline neuron system (1985) Gastroenterology, 89, pp. 118-126

Citas:

---------- APA ----------
Saravia-Fernandez, F., Faveeuw, C., Blasquez-Bulant, C., Tappaz, M., Throsby, M., Pelletier, G., Vaudry, H.,..., Homo-Delarche, F. (1996) . Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse. Endocrinology, 137(8), 3497-3506.
http://dx.doi.org/10.1210/endo.137.8.8754779
---------- CHICAGO ----------
Saravia-Fernandez, F., Faveeuw, C., Blasquez-Bulant, C., Tappaz, M., Throsby, M., Pelletier, G., et al. "Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse" . Endocrinology 137, no. 8 (1996) : 3497-3506.
http://dx.doi.org/10.1210/endo.137.8.8754779
---------- MLA ----------
Saravia-Fernandez, F., Faveeuw, C., Blasquez-Bulant, C., Tappaz, M., Throsby, M., Pelletier, G., et al. "Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse" . Endocrinology, vol. 137, no. 8, 1996, pp. 3497-3506.
http://dx.doi.org/10.1210/endo.137.8.8754779
---------- VANCOUVER ----------
Saravia-Fernandez, F., Faveeuw, C., Blasquez-Bulant, C., Tappaz, M., Throsby, M., Pelletier, G., et al. Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse. ENDOCRINOLOGY. 1996;137(8):3497-3506.
http://dx.doi.org/10.1210/endo.137.8.8754779