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Abstract:

The effects of long-term corticotropin-releasing hormone (CRH) infusion in the lateral ventricle of the rat on hypothalamic-pituitary-adrenocortical (HPA) axis parameters and on the immune system function were studied. Compared with infusion of vehicle, the CRH treatment produced a sustained overactivity of the HPA axis, as evidenced by elevated plasma ACTH and corticosterone levels, increased anterior pituitary POMC messenger RNA (mRNA) expression, and adrenal enlargement. Long-term CRH treatment also inhibited body weight gain and reduced thymus and spleen weight. In the CRH-treated animals, both Concanavalin A (Con A)-induced T lymphocyte proliferation and lipopolysaccharide (LPS)-induced B lymphocyte mitogenesis was largely suppressed. Surprisingly, interleukin-2 (IL-2) levels were higher in supernatants of splenocyte cultures from CRH-treated rats than in those of control animals. However, IL-2 receptor alpha chain (IL-2R α) mRNA expression after Con A stimulation was highly suppressed in the CRH-treated animals. In addition, Northern blot analysis of RNA from splenocytes isolated from spleens of CRH-treated rats revealed a marked expression of IL-1 β mRNA, in contrast to the barely detectable levels of this cytokine in control animals. Moreover, incubation of total splenocytes and spleen macrophages with LPS resulted in an enhanced induction of IL-1 β mRNA in cells of CRH-treated rats compared with that of control animals. When adrenalectomized rats were treated with CRH or vehicle, the effects of the CRH treatment on T and B cell proliferation, IL-2 production, and IL-1 β mRNA expression were abolished. Thus, a continuously increased HPA axis drive results in disparate changes in immune system function. Whether the observed changes in cytokine expression should be regarded as physiologically adaptive adjustments in support of immune function or as potentially pathological anomalies remains to be elucidated. © 1995 by The Endocrine Society.

Registro:

Documento: Artículo
Título:Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression
Autor:Labeur, M.S.; Arzt, E.; Wiegers, G.J.; Holsboer, F.; Reul, J.M.H.M.
Filiación:Max Planck Institute of Psychiatry, Clinical Institute, Department of Neuroendocrinology, Munich, 80804, Germany
Instituto de Investigaciones Médicas and Dto. Biología, Facultad de Ciencias Exactas y Naturales, Uniuersidad de Buenos Aires and Consejo National de Znvestigaciones Científicas y Técnicas, Buenos Aires, Argentina
Palabras clave:concanavalin a; corticosterone; corticotropin; corticotropin releasing factor; cytokine; interleukin 1beta; interleukin 2; interleukin 2 receptor; lipopolysaccharide; messenger rna; proopiomelanocortin; animal cell; animal experiment; article; b lymphocyte; corticotropin blood level; gene expression; hypothalamus hypophysis adrenal system; immune system; intracerebroventricular drug administration; lateral brain ventricle; lymphocyte proliferation; nonhuman; priority journal; rat; spleen cell; t lymphocyte; weight gain; Animal; B-Lymphocytes; Corticotropin-Releasing Hormone; Cytokines; Human; Hypothalamo-Hypophyseal System; In Vitro; Injections, Intraventricular; Interleukin-1; Interleukin-2; Lymphocyte Activation; Male; Neuroimmunomodulation; Pituitary-Adrenal System; Rats; Rats, Wistar; Receptors, Interleukin-2; RNA, Messenger; Support, Non-U.S. Gov't; T-Lymphocytes
Año:1995
Volumen:136
Número:6
Página de inicio:2678
Página de fin:2688
DOI: http://dx.doi.org/10.1210/endo.136.6.7750492
Título revista:Endocrinology
Título revista abreviado:Endocrinology
ISSN:00137227
CAS:concanavalin A, 11028-71-0; corticosterone, 50-22-6; corticotropin releasing factor, 9015-71-8; corticotropin, 11136-52-0, 9002-60-2, 9061-27-2; interleukin 2, 85898-30-2; proopiomelanocortin, 66796-54-1; Corticotropin-Releasing Hormone, 9015-71-8; Cytokines; Interleukin-1; Interleukin-2; Receptors, Interleukin-2; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v136_n6_p2678_Labeur

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Citas:

---------- APA ----------
Labeur, M.S., Arzt, E., Wiegers, G.J., Holsboer, F. & Reul, J.M.H.M. (1995) . Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression. Endocrinology, 136(6), 2678-2688.
http://dx.doi.org/10.1210/endo.136.6.7750492
---------- CHICAGO ----------
Labeur, M.S., Arzt, E., Wiegers, G.J., Holsboer, F., Reul, J.M.H.M. "Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression" . Endocrinology 136, no. 6 (1995) : 2678-2688.
http://dx.doi.org/10.1210/endo.136.6.7750492
---------- MLA ----------
Labeur, M.S., Arzt, E., Wiegers, G.J., Holsboer, F., Reul, J.M.H.M. "Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression" . Endocrinology, vol. 136, no. 6, 1995, pp. 2678-2688.
http://dx.doi.org/10.1210/endo.136.6.7750492
---------- VANCOUVER ----------
Labeur, M.S., Arzt, E., Wiegers, G.J., Holsboer, F., Reul, J.M.H.M. Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression. Endocrinology. 1995;136(6):2678-2688.
http://dx.doi.org/10.1210/endo.136.6.7750492