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The interleukin-1 (IL-1) system is constituted by IL-1 alpha and IL-1 β and IL-1 receptor antagonist (IL-1ra) that bind the same IL-1 receptors. Hypothalamic-pituitary-adrenal axis hormones are major mediators of the neuroendocrine control over immune function. Corticotropin-releasing hormone (CRH) is produced in peripheral inflammatory sites; its direct effects on inflammatory cytokine synthesis, however, remain unclear. We have studied the effects of CRH (0.1-100 nM) on IL-1 β and IL-1ra expression by human peripheral monocytes in culture activated with different doses of lipopolysaccharide (LPS). In the absence of LPS, CRH up-regulated IL-1ra and IL-1 β messenger RNA expression as well as protein synthesis. No significant changes were observed with low doses of LPS (1 ng/ml). In contrast, in combination with high doses of LPS (1 μ/ml), CRH caused inhibition of IL-1ra and IL-1 β transcription and secretion. The CRH effects were blocked by its antagonist alpha-helical CRH and mediated by intracellular cAMP. These data indicate that CRH modulates the IL-1 system; depending on the state of activation of the monocyte, CRH exerts an inhibitory control on the activated cell and a stimulatory action on the resting monocyte. © 1995 by The Endocrine Society.


Documento: Artículo
Título:Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin
Autor:Pereda, M.P.; Sauer, J.; Castro, C.P.; Finkielman, S.; Stalla, G.K.; Holsboer, F.; Arzt, E.
Filiación:Instituto de Investigaciones Médicas, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Biología-FCEN, Universidad de Buenos Aires, Buenos Aires, Argentina
Max-Planck Institute of Psychiatry, Clinical Institute, Munich, 80804, Germany
Palabras clave:corticotropin releasing factor; interleukin 1; interleukin receptor; article; corticotropin release; human; human cell; hypothalamus hypophysis adrenal system; leukocyte activation; priority journal; protein synthesis; Cells, Cultured; Corticotropin-Releasing Hormone; Cyclic AMP; Gene Expression Regulation; Human; Interleukin-1; Lipopolysaccharides; Monocytes; RNA, Messenger; Sialoglycoproteins; Support, Non-U.S. Gov't
Página de inicio:5504
Página de fin:5510
Título revista:Endocrinology
Título revista abreviado:Endocrinology
CAS:corticotropin releasing factor, 9015-71-8; Corticotropin-Releasing Hormone, 9015-71-8; Cyclic AMP, 60-92-4; interleukin 1 receptor antagonist protein; Interleukin-1; Lipopolysaccharides; RNA, Messenger; Sialoglycoproteins


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---------- APA ----------
Pereda, M.P., Sauer, J., Castro, C.P., Finkielman, S., Stalla, G.K., Holsboer, F. & Arzt, E. (1995) . Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin. Endocrinology, 136(12), 5504-5510.
---------- CHICAGO ----------
Pereda, M.P., Sauer, J., Castro, C.P., Finkielman, S., Stalla, G.K., Holsboer, F., et al. "Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin" . Endocrinology 136, no. 12 (1995) : 5504-5510.
---------- MLA ----------
Pereda, M.P., Sauer, J., Castro, C.P., Finkielman, S., Stalla, G.K., Holsboer, F., et al. "Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin" . Endocrinology, vol. 136, no. 12, 1995, pp. 5504-5510.
---------- VANCOUVER ----------
Pereda, M.P., Sauer, J., Castro, C.P., Finkielman, S., Stalla, G.K., Holsboer, F., et al. Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin. Endocrinology. 1995;136(12):5504-5510.