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Granulosa cells from diethylstilbestrol-treated immature rats were cultured in a defined medium on collagen-coated plates. Thymidine incorporation was significantly increased by insulin (ED50, 656 ±110 ng/ml) and insulin-like growth factor (IGF-I; ED50, 95 ± 10 ng/ml). Insulin and IGF-I stimulations were amplified by methylisobutylxan- thine an inhibitor of phosphodiesterase activity. The effect of both peptides were also enhanced by low doses of (Bu)2cAMP (0.2-1 mM). In contrast, higher concentrations were inhibitory. Similarly, FSH produced a biphasic enhancement of the insulin- and IGF-I-stimulated DNA synthesis. Maximal effects (2- to 6-fold increases) were observed with the lower doses (2-20 ng/ml) of the gonadotropin. FSH enhancement of IGF-I-stimulated DNA synthesis was dependent on cell density. Plating densities of 3-5 X 105 cells/cm2 were required for a maximal interaction. It is concluded that FSH, acting through a cAMP-mediated pathway, may regulate granulosa cell proliferation by modulating the mitogenic effects of insulin and/or IGF-I. © 1992 by The Endocrine Society.


Documento: Artículo
Título:Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis
Autor:Bley, M.A.; Simón, J.C.; Estévez, A.G.; De Asúa, L.J.; Barañao, J.L.
Filiación:Instituto de Biología y Medicina Experimental, Instituto de Investigaciones en Ingeniería Genètica y Biología Molecular, Facultad de Ciencias Exactas y Naturales, U.B.A, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:dna; follitropin; somatomedin c; animal cell; article; cell growth; dna synthesis; enzyme activity; granulosa cell; growth stimulation; hormone action; nonhuman; ovary follicle maturation; priority journal; rat; 1-Methyl-3-isobutylxanthine; Animal; Bucladesine; Cell Division; Cell Nucleus; Cells, Cultured; Cyclic AMP; DNA; DNA Replication; Female; Follicle Stimulating Hormone; Granulosa Cells; Insulin-Like Growth Factor I; Kinetics; Rats; Rats, Inbred Strains; Recombinant Proteins; Support, Non-U.S. Gov't
Página de inicio:1223
Página de fin:1239
Título revista:Endocrinology
Título revista abreviado:Endocrinology
CAS:1-Methyl-3-isobutylxanthine, 28822-58-4; Bucladesine, 362-74-3; Cyclic AMP, 60-92-4; DNA, 9007-49-2; Follicle Stimulating Hormone, 9002-68-0; Insulin-Like Growth Factor I, 67763-96-6; Recombinant Proteins


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---------- APA ----------
Bley, M.A., Simón, J.C., Estévez, A.G., De Asúa, L.J. & Barañao, J.L. (1992) . Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis. Endocrinology, 131(3), 1223-1239.
---------- CHICAGO ----------
Bley, M.A., Simón, J.C., Estévez, A.G., De Asúa, L.J., Barañao, J.L. "Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis" . Endocrinology 131, no. 3 (1992) : 1223-1239.
---------- MLA ----------
Bley, M.A., Simón, J.C., Estévez, A.G., De Asúa, L.J., Barañao, J.L. "Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis" . Endocrinology, vol. 131, no. 3, 1992, pp. 1223-1239.
---------- VANCOUVER ----------
Bley, M.A., Simón, J.C., Estévez, A.G., De Asúa, L.J., Barañao, J.L. Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis. Endocrinology. 1992;131(3):1223-1239.