Artículo
Cardinali, D.P.; Ritta, M.N.; Pereyra, E.; Solveyra, C.G. "Role of prostaglandins in rat pineal neuroeffector junction. changes in melatonin and norepinephrine release in vitro" (1982) Endocrinology. 111(2):530-534
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Abstract:
The effects of prostaglandins (PGs) on melatonin secretion and norepinephrine (NE) release in rat pineal gland were examined in vitro. To study melatonin secretion, pineal explants were incubated for 6 h in tissue culture 199 medium with 1–1000 nM PGE1, PGE2) or PGF2n. Melatonin concentration in pineal glands and media was determined by RIA. PGE2 increased pineal and medium melatonin at all concentrations tested, with a maximum of 1 nM; PGE1 was effective only at concentrations 100–1000 times greater, whereas 100 nM PGF2n decreased melatonin concentration in media but not in pineal gland. Exposure of pineal explants to 10 μM NE brought about a 20-fold increase in melatonin release to the medium. This effect was impaired significantly, but not blocked, by prior exposure to indomethacin, acetylsalicylic acid, or mefenamic acid at supramaximal concentrations to inhibit PG synthesis (100 μM). TO examine the effects of PGs on NE release, endogenous NE stores in pineal nerve endings were labeled in vitro by incubating rat pineals with [3H]NE for 30 min. Fifty minutes later, at the time when spontaneous radioactivity efflux had leveled off, transmitter release was elicited by a 1-min exposure to 80 mM K+ (S1), and the stimulus was repeated 35 min later (S2). PGs (10–100 nM) were added to the medium 20 min before S2. Ratios between fractional release of the two consecutive stimulations (S2/S1) varied between 0.84 and 1.16 in control pineals. Only 100 nM PGE2 impaired significantly transmitter release by 40%. These results suggest that PGE2 can play a role in NE-stimulated melatonin synthesis. At greater concentrations PGE2 inhibits NE release from pineal nerve endings. © 1982 by The Endocrine Society.
Registro:
| Documento: | Artículo |
| Título: | Role of prostaglandins in rat pineal neuroeffector junction. changes in melatonin and norepinephrine release in vitro |
| Autor: | Cardinali, D.P.; Ritta, M.N.; Pereyra, E.; Solveyra, C.G. |
| Filiación: | Centro de Estudios Farmacológicos y de Principios Naturales, Serrano 665/9, Buenos Aires, 1414, Argentina Consejo Nacional de Investigaciones Cientificas y Tácnicas (CONICET), Argentina CONICET, Argentina |
| Palabras clave: | acetylsalicylic acid; indometacin; mefenamic acid; melatonin; noradrenalin; potassium; prostaglandin; prostaglandin E; prostaglandin E1; prostaglandin E2; prostaglandin F; prostaglandin F2 alpha; animal; article; drug effect; male; metabolism; pineal body; rat; rat strain; Alprostadil; Animal; Aspirin; Dinoprost; Dinoprostone; Indomethacin; Male; Mefenamic Acid; Melatonin; Norepinephrine; Pineal Gland; Potassium; Prostaglandins; Prostaglandins E; Prostaglandins F; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't |
| Año: | 1982 |
| Volumen: | 111 |
| Número: | 2 |
| Página de inicio: | 530 |
| Página de fin: | 534 |
| DOI: | http://dx.doi.org/10.1210/endo-111-2-530 |
| Título revista: | Endocrinology |
| Título revista abreviado: | Endocrinology |
| ISSN: | 00137227 |
| CAS: | acetylsalicylic acid, 493-53-8, 50-78-2, 53663-74-4, 53664-49-6, 63781-77-1; indometacin, 53-86-1, 74252-25-8, 7681-54-1; mefenamic acid, 61-68-7; melatonin, 73-31-4; noradrenalin, 1407-84-7, 51-41-2; potassium, 7440-09-7; prostaglandin E, 11042-70-9; prostaglandin E1, 745-65-3; prostaglandin E2, 363-24-6; prostaglandin F2 alpha, 551-11-1; Alprostadil, 745-65-3; Aspirin, 50-78-2; Dinoprost, 551-11-1; Dinoprostone, 363-24-6; Indomethacin, 53-86-1; Mefenamic Acid, 61-68-7; Melatonin, 73-31-4; Norepinephrine, 51-41-2; Potassium, 7440-09-7; Prostaglandins E; Prostaglandins F; Prostaglandins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v111_n2_p530_Cardinali |
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Citas:
---------- APA ----------
Cardinali, D.P., Ritta, M.N., Pereyra, E. & Solveyra, C.G. (1982) . Role of prostaglandins in rat pineal neuroeffector junction. changes in melatonin and norepinephrine release in vitro. Endocrinology, 111(2), 530-534.http://dx.doi.org/10.1210/endo-111-2-530
---------- CHICAGO ----------
Cardinali, D.P., Ritta, M.N., Pereyra, E., Solveyra, C.G. "Role of prostaglandins in rat pineal neuroeffector junction. changes in melatonin and norepinephrine release in vitro" . Endocrinology 111, no. 2 (1982) : 530-534.http://dx.doi.org/10.1210/endo-111-2-530
---------- MLA ----------
Cardinali, D.P., Ritta, M.N., Pereyra, E., Solveyra, C.G. "Role of prostaglandins in rat pineal neuroeffector junction. changes in melatonin and norepinephrine release in vitro" . Endocrinology, vol. 111, no. 2, 1982, pp. 530-534.http://dx.doi.org/10.1210/endo-111-2-530
---------- VANCOUVER ----------
Cardinali, D.P., Ritta, M.N., Pereyra, E., Solveyra, C.G. Role of prostaglandins in rat pineal neuroeffector junction. changes in melatonin and norepinephrine release in vitro. Endocrinology. 1982;111(2):530-534.http://dx.doi.org/10.1210/endo-111-2-530
