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Abstract:

The binding and uptake of [3H]estradiol were studied in the anterior pituitary (AP) and hypothalamus of ovariectomized rats which were subjected to electrolytic destruction of the median eminence or to sham operation. As expected, serum PRL levels were markedly increased while LH was low in the lesioned rats. [3H]Estradiol binding in vitro by cytosol from the AP was decreased from a control value of 59.7 ± 6.2 to 36.9 ±6.1 fmol/mg protein in rats studied 8-10 days after placement of the lesion (P < 0.025). A time-course study, performed at various times after the lesion, indicated that binding of [3H]-estradiol in the AP was lower than normal when measured at 1 h and 1, 7, and 21 days. These changes were accompanied by increments in serum PRL throughout the experiment. Scatchard plot analysis of the binding data demonstrated a decreased binding capacity after median eminence lesion and an association constant in the range of the control AP. In contrast to the findings with the AP, median eminence lesion had no effect on [3H]estradiol binding in cytosol of hypothalamus. Intravenous administration of [3H]estradiol to control rats and rats lesioned 8 days previously demonstrated a significant lower uptake of radioactivity in the latter group in both AP and hypothalamus in vivo, regardless of whether results were expressed as the tissue to cortex ratio or as disintegrations per min/100 mg. The rates of [3H]glycine incorporation into proteins measured in incubated AP or hypothalamus were similar in controls and in rats with median eminence lesions, indicating that the decrement in binding or uptake was not due to an inhibition of total protein synthesis. These results suggest that estrogen receptors in the AP may be influenced by the brain, either by a direct trophic action of the hypothalamus or indirectly by regulation of serum PRL levels. © 1979 by The Endocrine Society.

Registro:

Documento: Artículo
Título:Effect of median eminence lesions on [3h]estradiol binding in the anterior pituitary and hypothalamus
Autor:Weisenberg, L.S.; De Nicola, A.F.; Arakelian, M.C.; Libertun, C.
Filiación:Laboratorios de Esteroides y Neuroendocrinología, Instituto de Biología y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:androgen; dexamethasone; estrogen; progesterone; prolactin; radioisotope; sex hormone binding protein; adenohypophysis; animal experiment; brain injury; central nervous system; endocrine system; estradiol h 3; glycine h 3; hormone binding; hypophysis; hypothalamus; median eminence; ovariectomy; rat; Animal; Brain; Comparative Study; Cytosol; Estradiol; Female; Hypothalamo-Hypophyseal System; Hypothalamus; Kinetics; Median Eminence; Organ Specificity; Pituitary Gland, Anterior; Proteins; Rats; Receptors, Estrogen
Año:1979
Volumen:105
Número:5
Página de inicio:1152
Página de fin:1157
DOI: http://dx.doi.org/10.1210/endo-105-5-1152
Título revista:Endocrinology
Título revista abreviado:Endocrinology
ISSN:00137227
CAS:dexamethasone, 50-02-2; progesterone, 57-83-0; prolactin, 12585-34-1, 50647-00-2, 9002-62-4; Estradiol, 50-28-2; Proteins; Receptors, Estrogen
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v105_n5_p1152_Weisenberg

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Citas:

---------- APA ----------
Weisenberg, L.S., De Nicola, A.F., Arakelian, M.C. & Libertun, C. (1979) . Effect of median eminence lesions on [3h]estradiol binding in the anterior pituitary and hypothalamus. Endocrinology, 105(5), 1152-1157.
http://dx.doi.org/10.1210/endo-105-5-1152
---------- CHICAGO ----------
Weisenberg, L.S., De Nicola, A.F., Arakelian, M.C., Libertun, C. "Effect of median eminence lesions on [3h]estradiol binding in the anterior pituitary and hypothalamus" . Endocrinology 105, no. 5 (1979) : 1152-1157.
http://dx.doi.org/10.1210/endo-105-5-1152
---------- MLA ----------
Weisenberg, L.S., De Nicola, A.F., Arakelian, M.C., Libertun, C. "Effect of median eminence lesions on [3h]estradiol binding in the anterior pituitary and hypothalamus" . Endocrinology, vol. 105, no. 5, 1979, pp. 1152-1157.
http://dx.doi.org/10.1210/endo-105-5-1152
---------- VANCOUVER ----------
Weisenberg, L.S., De Nicola, A.F., Arakelian, M.C., Libertun, C. Effect of median eminence lesions on [3h]estradiol binding in the anterior pituitary and hypothalamus. Endocrinology. 1979;105(5):1152-1157.
http://dx.doi.org/10.1210/endo-105-5-1152