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Abstract:

Trametes trogii laccase has been studied as biocatalyst for the oxygen electro-reduction in three different systems: (i) soluble laccase was studied in solution; (ii) an enzyme monolayer was tethered to a gold surface by dithiobis N-succinimidyl propionate (DTSP), with a soluble osmium pyridine-bipyridine redox mediator in both cases. The third case (iii) consisted in the sequential immobilization of laccase and the osmium complex derivatized poly(allylamine) self-assembled layer-by-layer (LbL) on mercaptopropane sulfonate modified gold to produce an all integrated and wired enzymatic oxygen cathode. The polycation was the same osmium complex covalently bound to poly-(ally-lamine) backbone (PAH-Os), the polyanion was the enzyme adsorbed from a solution of a suitable pH so that the protein carries a net negative charge. The adsorption of laccase was studied by monitoring the mass uptake with a quartz crystal microbalance and the oxygen reduction electrocatalysis was studied by linear scan voltammetry. While for the three cases, oxygen electrocatalysis mediated by the osmium complex was observed, for tethered laccase direct electron transfer in the absence of redox mediator was also apparent but no electrocatalysis for the oxygen reduction was recorded in the absence of mediator in solution. For the fully integrated LbL self-assembled laccase and redox mediator (case iii) a catalytic reduction of oxygen could be recorded at different oxygen partial pressures and different electrolyte pH. The tolerance of the reaction to methanol and chloride was also investigated. © 2008 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator
Autor:Szamocki, R.; Flexer, V.; Levin, L.; Forchiasin, F.; Calvo, E.J.
Filiación:INQUIMAE-DQIAyQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Micología Experimental, Departamento de Biodiversidad, Biología Experimental. Facultad de Ciencias Exactas y Naturales, 1428 Buenos Aires, Argentina
Palabras clave:Electrocatalysis; Laccase; Layer-by-layer self-assembly; Oxygen reducing cathode; Redox polymers; Adsorption; Aviation; Catalysis; Chlorine compounds; Electrocatalysis; Electrochemistry; Electrolysis; Electrolytic reduction; Enzymes; Gold compounds; Methanol; Nonmetals; Osmium; Oxide minerals; Oxygen; Quartz; Quartz crystal microbalances; Self assembly; Bipyridine; Catalytic reductions; Covalently bounds; Direct electron transfers; Electro reductions; Fully integrated; Gold surfaces; Laccase; Layer by layers; Layer-by-layer self-assembly; Linear-scan voltammetries; Negative charges; Osmium complexes; Oxygen cathodes; Oxygen partial pressures; Oxygen reducing cathode; Oxygen reductions; Poly(allylamine); Polyanion; Polycation; Quartz crystals; Redox mediators; Redox polymers; Self-assembled; Gas adsorption
Año:2009
Volumen:54
Número:7
Página de inicio:1970
Página de fin:1977
DOI: http://dx.doi.org/10.1016/j.electacta.2008.09.002
Título revista:Electrochimica Acta
Título revista abreviado:Electrochim Acta
ISSN:00134686
CODEN:ELCAA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00134686_v54_n7_p1970_Szamocki

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Citas:

---------- APA ----------
Szamocki, R., Flexer, V., Levin, L., Forchiasin, F. & Calvo, E.J. (2009) . Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator. Electrochimica Acta, 54(7), 1970-1977.
http://dx.doi.org/10.1016/j.electacta.2008.09.002
---------- CHICAGO ----------
Szamocki, R., Flexer, V., Levin, L., Forchiasin, F., Calvo, E.J. "Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator" . Electrochimica Acta 54, no. 7 (2009) : 1970-1977.
http://dx.doi.org/10.1016/j.electacta.2008.09.002
---------- MLA ----------
Szamocki, R., Flexer, V., Levin, L., Forchiasin, F., Calvo, E.J. "Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator" . Electrochimica Acta, vol. 54, no. 7, 2009, pp. 1970-1977.
http://dx.doi.org/10.1016/j.electacta.2008.09.002
---------- VANCOUVER ----------
Szamocki, R., Flexer, V., Levin, L., Forchiasin, F., Calvo, E.J. Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator. Electrochim Acta. 2009;54(7):1970-1977.
http://dx.doi.org/10.1016/j.electacta.2008.09.002