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Tesone, M.; Oliveira-Filho, R.M.; Biella de Souza Valle, L.; Calvo, J.C.; Barañao, J.L.S.; Foglia, V.G.; Charreau, E.H. "Androgen receptors in the diabetic rat" (1980) Diabetologia. 18(5):385-390
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Abstract:

Male rats rendered diabetic by IV streptozotocin (65mg/kg body weight) were treated with exogenous insulin or testosterone. Charcoal-coated dextran and polyacrylemide gel electrophoresis techniques were employed in studying the characteristics of androgen (R1881) binding to prostate cytosol protein. In comparison with normal (N) rats, the replacement therapy of diabetic (D) animals with insulin (D+I) or testosterone (D+T) was able to restore epididymal weight (N = 0.40 ±0.04 g; D = 0.18 ± 0.02 g; D+I = 0.42 ± 0.05 g; D+T = 0.40 ± 0.06 g) and total prostate weight (N = 0.24 ± 0.02 g; D = 0.15 ± 0.02 g; D+I = 0.24 ± 0.05 g; D+T = 0.35 ± 0.06 g). Testicular endogenous content of testosterone was restored after insulin treatment (N = 154 ± 13 ng/testis; D = 41 ± 5 ng/testis; D+I = 142 ± 9 ng/testis), and significant improvements of serum testosterone levels were also achieved (N = 540 ± 64 ng/100 ml; D = 238 ± 37 ng/100 ml; D+I = 358 ± 18 ng/100 ml). Prostate cytosol of streptozotocin-diabetic rats had strongly lowered capacity for 3H-R1881 binding compared with controls (94 and 12 fmol/mg protein, respectively). Testosterone treatment produced a 3.3-fold improvement of this lowered value, whereas the increment seen with insulin was less (1.5-fold). It is emphasized that some of the improvements caused by insulin replacement therapy in diabetic animals are due to the partial restoration of testosterone secretion. Thus, the combined actions of insulin and testosterone (instead of insulin alone) seem to be of major importance in the maintenance and regulation of accessory sex glands function. © 1980 Springer-Verlag.

Registro:

Documento: Artículo
Título:Androgen receptors in the diabetic rat
Autor:Tesone, M.; Oliveira-Filho, R.M.; Biella de Souza Valle, L.; Calvo, J.C.; Barañao, J.L.S.; Foglia, V.G.; Charreau, E.H.
Filiación:Laboratorio de Esteroides, Instituto de Biología y Medicina Experimental, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Androgen receptors; cytosol binding sites; R1881; rat prostate; streptozotocin-diabetes; androgen receptor; androstanolone; androstanolone h 3; drug receptor; insulin; methyltrienolone h 3; metribolone; radioisotope; streptozocin; testosterone; unclassified drug; animal experiment; article; diabetes mellitus; endocrine system; male genital system; prostate; rat; Animals; Body Weight; Cytosol; Diabetes Mellitus, Experimental; Epididymis; Estrenes; Insulin, Long-Acting; Male; Metribolone; Organ Size; Prostate; Rats; Receptors, Androgen; Receptors, Steroid; Testis; Testosterone; Testosterone Congeners
Año:1980
Volumen:18
Número:5
Página de inicio:385
Página de fin:390
DOI: http://dx.doi.org/10.1007/BF00276819
Título revista:Diabetologia
Título revista abreviado:Diabetologia
ISSN:0012186X
CODEN:DBTGA
CAS:androstanolone, 521-18-6; insulin, 9004-10-8; metribolone, 965-93-5; streptozocin, 18883-66-4; testosterone, 58-22-0; Estrenes; Insulin, Long-Acting; Metribolone, 965-93-5; Receptors, Androgen; Receptors, Steroid; Testosterone, 58-22-0; Testosterone Congeners
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0012186X_v18_n5_p385_Tesone

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Citas:

---------- APA ----------
Tesone, M., Oliveira-Filho, R.M., Biella de Souza Valle, L., Calvo, J.C., Barañao, J.L.S., Foglia, V.G. & Charreau, E.H. (1980) . Androgen receptors in the diabetic rat. Diabetologia, 18(5), 385-390.
http://dx.doi.org/10.1007/BF00276819
---------- CHICAGO ----------
Tesone, M., Oliveira-Filho, R.M., Biella de Souza Valle, L., Calvo, J.C., Barañao, J.L.S., Foglia, V.G., et al. "Androgen receptors in the diabetic rat" . Diabetologia 18, no. 5 (1980) : 385-390.
http://dx.doi.org/10.1007/BF00276819
---------- MLA ----------
Tesone, M., Oliveira-Filho, R.M., Biella de Souza Valle, L., Calvo, J.C., Barañao, J.L.S., Foglia, V.G., et al. "Androgen receptors in the diabetic rat" . Diabetologia, vol. 18, no. 5, 1980, pp. 385-390.
http://dx.doi.org/10.1007/BF00276819
---------- VANCOUVER ----------
Tesone, M., Oliveira-Filho, R.M., Biella de Souza Valle, L., Calvo, J.C., Barañao, J.L.S., Foglia, V.G., et al. Androgen receptors in the diabetic rat. Diabetologia. 1980;18(5):385-390.
http://dx.doi.org/10.1007/BF00276819