Artículo

Da Ros, V.G.; Maldera, J.A.; Willis, W.D.; Cohen, D.J.; Goulding, E.H.; Gelman, D.M.; Rubinstein, M.; Eddy, E.M.; Cuasnicu, P.S. "Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)" (2008) Developmental Biology. 320(1):12-18
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Abstract:

Mammalian fertilization is a complex multi-step process mediated by different molecules present on both gametes. Epididymal protein CRISP1, a member of the Cysteine-RIch Secretory Protein (CRISP) family, was identified by our laboratory and postulated to participate in both sperm-zona pellucida (ZP) interaction and gamete fusion by binding to egg-complementary sites. To elucidate the functional role of CRISP1 in vivo, we disrupted the Crisp1 gene and evaluated the effect on animal fertility and several sperm parameters. Male and female Crisp1-/- animals exhibited no differences in fertility compared to controls. Sperm motility and the ability to undergo a spontaneous or progesterone-induced acrosome reaction were neither affected in Crisp1-/- mice. However, the level of protein tyrosine phosphorylation during capacitation was clearly lower in mutant sperm than in controls. In vitro fertilization assays showed that Crisp1-/- sperm also exhibited a significantly reduced ability to penetrate both ZP-intact and ZP-free eggs. Moreover, when ZP-free eggs were simultaneously inseminated with Crisp1+/+ and Crisp1-/- sperm in a competition assay, the mutant sperm exhibited a greater disadvantage in their fusion ability. Finally, the finding that the fusion ability of Crisp1-/- sperm was further inhibited by the presence of CRISP1 or CRISP2 during gamete co-incubation, supports that another CRISP cooperates with CRISP1 during fertilization and might compensate for its lack in the mutant mice. Together, these results indicate that CRISP proteins are players in the mammalian fertilization process. To our knowledge this is the first knockout mice generated for a CRISP protein. The information obtained might have important functional implications for other members of the widely distributed and evolutionarily conserved CRISP family. © 2008 Elsevier Inc.

Registro:

Documento: Artículo
Título:Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)
Autor:Da Ros, V.G.; Maldera, J.A.; Willis, W.D.; Cohen, D.J.; Goulding, E.H.; Gelman, D.M.; Rubinstein, M.; Eddy, E.M.; Cuasnicu, P.S.
Filiación:Instituto de Biología y Medicina Experimental (IBYME-CONICET), Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina
Gamete Biology Section, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, United States
Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI-CONICET), Buenos Aires, 1428, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
Palabras clave:CRISP; Egg; Fertilization; Sperm; cysteine rich secretory protein 1; DNA; RNA; secretory protein; tyrosine; acrosome reaction; animal cell; animal experiment; animal tissue; article; controlled study; female; fertility; fertilization; fertilization in vitro; gene disruption; genotype; immunoblotting; male; mouse; mutant; nonhuman; priority journal; protein phosphorylation; reverse transcription polymerase chain reaction; sperm; spermatozoon capacitation; spermatozoon motility; zona pellucida; Animalia; Mammalia; Mus
Año:2008
Volumen:320
Número:1
Página de inicio:12
Página de fin:18
DOI: http://dx.doi.org/10.1016/j.ydbio.2008.03.015
Handle:http://hdl.handle.net/20.500.12110/paper_00121606_v320_n1_p12_DaRos
Título revista:Developmental Biology
Título revista abreviado:Dev. Biol.
ISSN:00121606
CODEN:DEBIA
CAS:DNA, 9007-49-2; RNA, 63231-63-0; tyrosine, 16870-43-2, 55520-40-6, 60-18-4
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00121606_v320_n1_p12_DaRos.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00121606_v320_n1_p12_DaRos

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Citas:

---------- APA ----------
Da Ros, V.G., Maldera, J.A., Willis, W.D., Cohen, D.J., Goulding, E.H., Gelman, D.M., Rubinstein, M.,..., Cuasnicu, P.S. (2008) . Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1). Developmental Biology, 320(1), 12-18.
http://dx.doi.org/10.1016/j.ydbio.2008.03.015
---------- CHICAGO ----------
Da Ros, V.G., Maldera, J.A., Willis, W.D., Cohen, D.J., Goulding, E.H., Gelman, D.M., et al. "Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)" . Developmental Biology 320, no. 1 (2008) : 12-18.
http://dx.doi.org/10.1016/j.ydbio.2008.03.015
---------- MLA ----------
Da Ros, V.G., Maldera, J.A., Willis, W.D., Cohen, D.J., Goulding, E.H., Gelman, D.M., et al. "Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)" . Developmental Biology, vol. 320, no. 1, 2008, pp. 12-18.
http://dx.doi.org/10.1016/j.ydbio.2008.03.015
---------- VANCOUVER ----------
Da Ros, V.G., Maldera, J.A., Willis, W.D., Cohen, D.J., Goulding, E.H., Gelman, D.M., et al. Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1). Dev. Biol. 2008;320(1):12-18.
http://dx.doi.org/10.1016/j.ydbio.2008.03.015