Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Rat epididymal protein DE is localized on the fusogenic region of the acrosome-reacted spermatozoa and has a potential role in sperm-egg fusion. We investigated the presence of DE binding sites on the egg surface by co-incubating zona-free eggs and capacitated sperm in different concentrations of pure DE. Results indicate that DE produced a concentration-dependent decrease in egg penetration by sperm (fusion), with almost complete inhibition at 200 μg/ml. This inhibition was not due to an effect of DE on initial sperm binding to the egg membrane, since the presence of this protein did not affect the percentage of oocytes with bound sperm nor the number of bound sperm per egg. Those sperm that failed to penetrate the egg in the presence of DE became able to do so after transfer of the eggs to protein- and sperm-free medium, indicating a role for DE in an event subsequent to binding and leading to fusion. Indirect immunofluorescence using a polyclonal antibody against DE revealed a patchy labeling over the entire egg surface, with the exception of the area overlying the second metaphase spindle. This conclusion was supported by the disappearance of the DE-negative area on the fertilized egg. Zona-free eggs, incubated with DE at 4°C or fixed before exposure to DE displayed a uniform staining, suggesting that the patchy labeling resulted from aggregation of DE binding sites by the purified protein. The aggregation of these egg components may represent a necessary step of the fusion process. To our knowledge, this is the first study reporting the existence and localization of complementary sites to a specific sperm protein on the plasma membrane of the mammalian egg. © 1992.

Registro:

Documento: Artículo
Título:Mammalian sperm-egg fusion: The rat egg has complementary sites for a sperm protein that mediates gamete fusion
Autor:Rochwerger, L.; Cohen, D.J.; Cuasnicú, P.S.
Filiación:Instituto de Biología y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:protein; animal cell; article; cell fusion; egg; female; fertilization; gamete; male; nonhuman; oocyte; priority journal; rat; spermatozoon; Amino Acid Sequence; Animal; Binding Sites; Female; Glycoproteins; Male; Membrane Fusion; Membrane Glycoproteins; Molecular Sequence Data; Ovum; Rats; Rats, Inbred Strains; Sperm-Ovum Interactions; Spermatozoa; Support, Non-U.S. Gov't; Animalia; Mammalia; Mammalia
Año:1992
Volumen:153
Número:1
Página de inicio:83
Página de fin:90
DOI: http://dx.doi.org/10.1016/0012-1606(92)90093-V
Título revista:Developmental Biology
Título revista abreviado:Dev. Biol.
ISSN:00121606
CODEN:DEBIA
CAS:Glycoproteins; Membrane Glycoproteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00121606_v153_n1_p83_Rochwerger

Referencias:

  • Boldt, Howe, Preble, Enzymatic alteration of the ability of mouse egg plasma membrane to interact with sperm (1988) Biol. Reprod, 39, pp. 19-27
  • Boldt, Gunter, Howe, Characterization of cell surface polypeptides of unfertilized, fertilized, and protease-treated zona-free mouse eggs (1989) Gamete Res, 23, pp. 91-101
  • Brett-Finlay, Heffron, Falkow, Epithelial cell surfaces induce Salmonella proteins required for bacterial adherence and invasion (1989) Science, 243, pp. 940-943
  • Bronson, Fusi, Sperm-oolemal interaction: Role of the Arg-Gly-Asp (RGD) adhesion peptide (1990) Fertil. Steril, 54, pp. 527-529
  • Bronson, Fusi, Evidence that an Arg-Gly-Asp adhesion sequence plays a role in mammalian fertilization (1990) Biol. Reprod, 43, pp. 1019-1025
  • Cameo, Gonzalez Echeverria, Blaquier, Burgos, Immunochemical localization of epididymal protein DE on rat spermatozoa: Its fate after induced acrosome reaction (1986) Gamete Res, 15, pp. 247-258
  • Chandler, Comparison of quick-frozen and chemically fixed sea urchin eggs: Structural evidence that cortical granule exocytosis is preceded by a local increase in membrane mobility (1984) J. Cell Sci, 72, pp. 23-36
  • Cuasnicú, Gonzalez Echeverria, Piazza, Cameo, Blaquier, Antibody against epididymal glycoprotein blocks fertilizing ability in rats (1984) Reproduction, 72, pp. 467-471
  • Cuasnicú, Conesa, Rochwerger, Potential contraceptive use of an epididymal protein that participates in fertilization (1990) Gamete Interaction: Prospects for Immunocontraception, pp. 143-154. , Wiley-Liss, New York
  • Detmers, Wright, Olsen, Kimball, Cohn, Aggregation of complement receptor on human neutrophils in the absence of ligand (1987) J. Cell Biol, 105, pp. 1137-1145
  • Eager, Johnson, Thurley, Ultrastructural studies on the surface membrane of the mouse egg (1976) J. Cell Sci, 22, pp. 345-353
  • Ebensperger, Barros, Changes at the hamster oocyte surface from the germinal vesicle stage to ovulation (1984) Gamete Res, 9, pp. 387-397
  • Foltz, Lennarz, Purification and characterization of an extracellular fragment of the sea urchin egg receptor for sperm (1990) J. Cell Biol, 111, pp. 2951-2959
  • Foltz, Lennarz, Identification of the sea urchin egg receptor for sperm using an antiserum raised against a fragment of its extracellular domain (1992) J. Cell Biol, 116, pp. 647-658
  • Garberi, Kohane, Cameo, Blaquier, Isolation and characterization of specific rat epididymal proteins (1979) Mol. Cell. Endocrinol, 13, pp. 73-82
  • Glabe, Interaction of the sperm adhesive protein, bindin, with phospholipid vesicles. II. Bindin induces the fusion of mixed-phase vesicles that contain phosphatidylcholine and phosphatidylserine in vitro (1985) J. Cell Biol, 100, pp. 800-806
  • Hirao, Yanagimachi, Temperature-dependence of sperm-egg fusion and post-fusion events in hamster fertilization (1978) J. Exp. Zool, 205, pp. 433-438
  • Ikari, Yoshino, Moses, Flier, Evidence that receptor aggregation may play a role in transmembrane signaling through the insulin-like growth factor-I receptor (1988) Mol. Endocrinol, 2, pp. 831-837
  • Johnson, Eager, Muggleton-Harris, Grave, Mosaicism in organization of concanavalin A receptors on surface membranes of mouse eggs (1975) Nature, 257, pp. 321-322
  • Kaplan, Kraicer, Effect of elevated calcium concentration on fertilization of rat oocytes in vitro (1978) Gamete Research, 1, pp. 281-285
  • Kennedy, DeAngelis, Glabe, Analysis of the membrane-interacting domain of the sea urchin sperm adhesive protein bindin (1989) Biochemistry, 28, pp. 9153-9158
  • Knudsen, The calcium-dependent myoblast adhesion that precedes cell fusion is mediated by glycoproteins (1985) J. Cell Biol, 101, pp. 891-897
  • Kohane, González Echeverría, Piñeiro, Blaquier, Interaction of proteins of epididymal origin with spermatozoa (1980) Biol. Reprod, 23, pp. 737-742
  • Kohane, Cameo, Piñeiro, Garberi, Blaquier, Distribution and production of specific proteins in the rat epididymis (1980) Biol. Reprod, 23, pp. 181-187
  • Ledbetter, June, Grossmaire, Rabinovitch, Crosslinking of a surface antigens causes mobilization of intracellular ionized calcium in T lymphocytes (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 1384-1388
  • Leyton, Saling, Evidence that aggregation of mouse sperm receptors by ZP3 triggers the acrosome reaction (1989) J. Cell Biol, 108, pp. 2163-2168
  • Longo, Chen, Development of cortical polarity in mouse eggs: Involvement of the meiotic apparatus (1985) Dev. Biol, 107, pp. 382-394
  • Nicolson, Yanagimachi, Yanagimachi, Ultrastructural localization of lectin-binding sites on the zonae pellucidae and plasma membranes of mammalian eggs (1975) J. Cell Biol, 66, pp. 263-273
  • O'Brien, Soos, Siddle, Monoclonal antibodies to the insulin receptor stimulate the intrinsic tyrosine kinase activity by cross-linking receptor molecules (1987) EMBO J, 6, pp. 4003-4010
  • Phillips, Shalgi, Surface architecture of the mouse and hamster zona pellucida and oocyte (1980) J. Ultrastruct. Res, 72, pp. 1-12
  • Primakoff, Hyatt, Tredick-Kline, Identification and purification of a sperm surface protein with a potential role in sperm-egg membrane fusion (1987) J. Cell Biol, 104, pp. 141-149
  • Rochwerger, Cuasnicú, Redistribution of a rat sperm epididymal glycoprotein after in vivo and in vitro capacitation (1992) Mol. Reprod. Dev, 31, pp. 34-41
  • Saling, Irons, Waibel, Mouse sperm antigens that participate in fertilization. I. Inhibition of sperm fusion with the egg plasma membrane using monoclonal antibodies (1985) Biol. Reprod, 33, pp. 515-526
  • White, Membrane fusion proteins of enveloped animal viruses (1983) Quart. Rev. Biophys, 16 (2), pp. 151-195
  • White, Viral and cellular membrane fusion proteins (1990) Annu. Rev. Physiol, 52, pp. 675-697
  • Wolf, Ziomek, Regionalization and lateral diffusion of membrane proteins in unfertilized and fertilized mouse eggs (1983) J. Cell Biol, 96, pp. 1786-1790
  • Yanagimachi, Calcium requirement for sperm-egg fusion in mammals (1978) Biol. Reprod, 19, pp. 949-958
  • Yanagimachi, Mechanisms of fertilization in mammals (1981) Fertilization and Embryonic Development In Vitro, pp. 81-182. , L. Mastroianni, J.D. Biggers, Plenum Press, New York
  • Yanagimachi, Sperm-egg fusion (1988) Current Topics in Membranes and Transport, 32, pp. 3-43. , N. Duzgunes, F. Bronner, Academic Press, Orlando, FL

Citas:

---------- APA ----------
Rochwerger, L., Cohen, D.J. & Cuasnicú, P.S. (1992) . Mammalian sperm-egg fusion: The rat egg has complementary sites for a sperm protein that mediates gamete fusion. Developmental Biology, 153(1), 83-90.
http://dx.doi.org/10.1016/0012-1606(92)90093-V
---------- CHICAGO ----------
Rochwerger, L., Cohen, D.J., Cuasnicú, P.S. "Mammalian sperm-egg fusion: The rat egg has complementary sites for a sperm protein that mediates gamete fusion" . Developmental Biology 153, no. 1 (1992) : 83-90.
http://dx.doi.org/10.1016/0012-1606(92)90093-V
---------- MLA ----------
Rochwerger, L., Cohen, D.J., Cuasnicú, P.S. "Mammalian sperm-egg fusion: The rat egg has complementary sites for a sperm protein that mediates gamete fusion" . Developmental Biology, vol. 153, no. 1, 1992, pp. 83-90.
http://dx.doi.org/10.1016/0012-1606(92)90093-V
---------- VANCOUVER ----------
Rochwerger, L., Cohen, D.J., Cuasnicú, P.S. Mammalian sperm-egg fusion: The rat egg has complementary sites for a sperm protein that mediates gamete fusion. Dev. Biol. 1992;153(1):83-90.
http://dx.doi.org/10.1016/0012-1606(92)90093-V