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Abstract:

For cytogenetic studies it is necessary to develop an efficient method of chromosome preparation with wellpreserved and dispersed chromosomes and little or no cell-wall debris. In this study, we describe a method for obtaining high quality chromosomal preparations of dipteran cells. This procedure combines drop method and air-dry technique and produces a cell suspension with separated and mixed cells. Suspended mitotic cells were then dropped onto a glass slide in order to distribute the cytoplasm and spread the chromosomes. We compare and evaluate different chromosome preparation methods to develop a more reliable procedure for the resolution of chromosome characteristics and chromosome bandings in blow fly mitotic chromosomes. The combined drop and air-dry technique described here is convenient for identification of sex chromosomes. Using this protocol, acceptable high-quality chromosomal spreads with flattened cells and no cell debris, and without damage and/or loss of chromosomes were obtained.

Registro:

Documento: Artículo
Título:Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations
Autor:Chirino, M.G.; Rossi, L.F.; Bressa, M.J.; Luaces, J.P.; Merani, M.S.
Filiación:Instituto de Ecología, Genética y Evolución de Buenos Aires, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Laboratorio de Biología Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Calliphoridae; Diptera
Año:2014
Volumen:107
Número:11
Página de inicio:1792
Página de fin:1794
Título revista:Current Science
Título revista abreviado:Curr. Sci.
ISSN:00113891
CODEN:CUSCA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00113891_v107_n11_p1792_Chirino

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Citas:

---------- APA ----------
Chirino, M.G., Rossi, L.F., Bressa, M.J., Luaces, J.P. & Merani, M.S. (2014) . Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations. Current Science, 107(11), 1792-1794.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00113891_v107_n11_p1792_Chirino [ ]
---------- CHICAGO ----------
Chirino, M.G., Rossi, L.F., Bressa, M.J., Luaces, J.P., Merani, M.S. "Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations" . Current Science 107, no. 11 (2014) : 1792-1794.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00113891_v107_n11_p1792_Chirino [ ]
---------- MLA ----------
Chirino, M.G., Rossi, L.F., Bressa, M.J., Luaces, J.P., Merani, M.S. "Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations" . Current Science, vol. 107, no. 11, 2014, pp. 1792-1794.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00113891_v107_n11_p1792_Chirino [ ]
---------- VANCOUVER ----------
Chirino, M.G., Rossi, L.F., Bressa, M.J., Luaces, J.P., Merani, M.S. Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations. Curr. Sci. 2014;107(11):1792-1794.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00113891_v107_n11_p1792_Chirino [ ]