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Abstract:

The stabilizing role of sugars on dehydrated membranes is well established. The formation of a glassy matrix and the direct interaction between the sugars and the lipids are some of the mechanisms proposed to be involved in this stabilizing effect. Phospholipidic systems have been studied extensively as models for biological membranes and also due to the practical applications of liposomes as vehicles for drug delivery. In this work, we evaluate the effect of sugar-phosphate mixtures on the transition temperature of dehydrated 1,2-dipalmitoylphosphatidylcholine, and also examine some physical characteristics of these mixtures, such as the glass transition temperature and water sorption properties. The addition of phosphate salts to sugar systems has several interesting features that merit its consideration in formulations to protect dehydrated labile biomaterials. In particular, sucrose-phosphate mixtures provide an interesting alternative to pure saccharide formulations due to their high glass transition temperatures and their increased ability to maintain a low melting transition temperature in the presence of small amounts of water. © 2004 Published by Elsevier Inc.

Registro:

Documento: Artículo
Título:Effect of sugar-phosphate mixtures on the stability of DPPC membranes in dehydrated systems
Autor:Ohtake, S.; Schebor, C.; Palecek, S.P.; De Pablo, J.J.
Filiación:Dept. of Chem. and Biol. Engineering, University of Wisconsin, 1415 Engineering Drive, Madison, WI 53706, United States
Departamento de Industrias, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428 Cd. Auton. de Buenos Aires, Argentina
CONICET, Argentina
Palabras clave:Glass transition; Glasses; Lipids; Sucrose; Trehalose; Vacuum-drying; biomaterial; carbohydrate; choline derivative; dipalmitoylphosphatidylcholine; phosphate; phospholipid derivative; stabilizing agent; sugar; article; artificial membrane; chemical analysis; complex formation; glass transition temperature; melting point; molecular interaction; physical chemistry; priority journal
Año:2004
Volumen:48
Número:1
Página de inicio:81
Página de fin:89
DOI: http://dx.doi.org/10.1016/j.cryobiol.2004.01.001
Título revista:Cryobiology
Título revista abreviado:Cryobiology
ISSN:00112240
CODEN:CRYBA
CAS:dipalmitoylphosphatidylcholine, 2644-64-6; phosphate, 14066-19-4, 14265-44-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00112240_v48_n1_p81_Ohtake

Referencias:

  • Caffrey, M., Fonseca, V., Leopold, A.C., Lipid-sugar interactions: Relevance to anhydrous biology (1988) Plant Physiol., 86, pp. 754-758
  • Cardona, S., Schebor, C., Buera, M.P., Chirife, J., Thermal stability of invertase in reduced-moisture amorphous matrices in relation to glassy state and trehalose crystallization (1997) J. Food Sci., 62, pp. 105-110
  • Carpenter, J., Crowe, L.M., Crowe, J.H., Stabilization of phosphofructokinase with sugars during freeze-drying: Characterization of enhanced protection in the presence of divalent cations (1987) Biochim. Biophys. Acta, 923, pp. 109-115
  • Conrad, P.B., Miller, D.P., Cielenski, P.R., De Pablo, J.J., Stabilization and preservation of Lactobacillus acidophilus in saccharide matrices (2000) Cryobiology, 41, pp. 17-24
  • Crowe, J.H., Crowe, L.M., Chapman, D., Preservation of membranes in anhydrobiotic organisms: The role of trehalose (1984) Science, 223, pp. 701-703
  • Crowe, J.H., Crowe, L.M., Carpenter, J.F., Aurell Wistrom, C., Stabilization of dry phospholipid bilayers and proteins by sugars (1987) Biochem. J., 242, pp. 1-10
  • Crowe, J.H., Crowe, L.M., Preservation of liposomes by freeze-drying (1992) Liposome Technology, , G. Gregoriadis (Ed.), CRC Press, Boca Raton, FL
  • Crowe, J.H., Carpenter, J.F., Crowe, L.M., The role of vitrification in anhydrobiosis (1998) Annu. Rev. Physiol., 6, pp. 73-103
  • Crowe, L.M., Womersley, C., Crowe, J.H., Reid, D., Appel, L., Rudolph, A., Prevention of fusion and leakage in freeze-dried liposomes by carbohydrates (1986) Biochim. Biophys. Acta, 861, pp. 131-140
  • Crowe, L.M., Crowe, J.H., Trehalose and dipalmitoylphosphatidylcholine revisited (1988) Biochim. Biophys. Acta, 946, pp. 193-201
  • Crowe, L.M., Reid, D., Crowe, J.H., Is trehalose special for preserving dry biomaterials? (1996) Biophys. J., 71, pp. 2087-2093
  • Ekdawi-Sever, N., Conrad, P.B., De Pablo, J.J., The effects of annealing on freeze-dried Lactobacillus acidophilus (2003) J. Food Sci., 68, pp. 2504-2511
  • Engelsen, S.B., Du Penhoat, C.H., Perez, S., Molecular relaxation of sucrose in aqueous solution: How a nanosecond molecular dynamics simulation helps to reconcile NMR data (1998) J. Phys. Chem., 99, pp. 13334-13351
  • Gennis, R.B., (1989) Biomembranes: Molecular Structure and Function, , New York: Springer-Verlag
  • Greenspan, L., Humidity fixed points of binary saturated aqueous solutions (1977) J. Res., 81 (A), pp. 89-96
  • Hancock, B., Zografi, G., Characteristics and significance of the amorphous state in pharmaceutical systems (1997) J. Pharm. Sci., 86, pp. 1-12
  • Koster, K., Webb, M.S., Bryant, G., Lynch, D.V., Interactions between soluble sugars and POPC (1-palmitoyl-2- oleoylphosphatidylcholine) during dehydration: Vitrification of sugars alters the phase behavior of the phospholipid (1994) Biochim. Biophys. Acta, 1193, pp. 143-150
  • Koster, K., Lei, Y.P., Anderson, M., Martin, S., Bryant, G., Effects of vitrified and nonvitrified sugars on phosphatidylcholine fluid-to-gel phase transition (2000) Biophys. J., 78, pp. 1932-1946
  • Lee, J.C., Timasheff, S.N., The stabilization of proteins by sucrose (1981) J. Biol. Chem., 256, pp. 7193-7201
  • Leslie, S., Israeli, B., Lighthart, J., Crowe, J.H., Crowe, L.M., Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying (1995) Appl. Environ. Microbiol., 61, pp. 3592-3597
  • Longinotti, M.P., Mazzobre, M.F., Buera, M.P., Corti, H.R., Effect of salts on properties of aqueous sugar systems in relation to biomaterial stabilization. 2. Sugar crystallization rate and electrical conductivity behavior (2002) Phys. Chem. Chem. Phys., 4, pp. 533-540
  • MacFarlane, D.R., Pringle, J., Annat, G., Reversible self-polymerizing high Tg lyoprotectants (2002) Cryobiology, 45, pp. 188-192
  • Mazzobre, M.F., Buera, M.P., Chirife, J., Glass transition and thermal stability of lactase in low-moisture amorphous polymeric systems (1997) Biotechnol. Prog., 13, pp. 195-199
  • Mazzobre, M.F., Buera, P., Combined effects of trehalose and cations on thermal resistance of B-galactosidase in freeze-dried systems (1999) Biochim. Biophys. Acta, 1473, pp. 337-344
  • Mazzobre, M.F., Longinotti, M.P., Corti, H.R., Buera, M.P., Effect of salts on the properties of aqueous sugar systems, in relation to biomaterial stabilization. 1. Water sorption behaviour and ice crystallization/melting (2001) Cryobiology, 43, pp. 199-210
  • Miller, D.P., Anderson, R.E., De Pablo, J.J., Stabilization of lactate dehydrogenase following freeze-thawing and vacuum drying in the presence of trehalose and borate (1998) Pharm. Res., 15, pp. 1215-1221
  • Miller, D.P., De Pablo, J.J., Corti, H.R., Viscosity and glass transition temperature of aqueous mixtures of trehalose with borax and sodium chloride (1999) J. Phys. Chem. B, 103, p. 10243
  • Moreira, R., Pendas, J., Gutierrez, A., Pomes, R., Duque, J., Franks, F., Effect of sucrose and raffinose on physical state and on lactate dehydrogenase activity of freeze-dried formulations (1998) Cryo Letters, 19, pp. 115-122
  • Murakami, R., Motozato, Y., Viscosity of aqueous glucomannan-borate complex solutions (1992) Polymer, 33, pp. 1108-1109
  • Nagase, H., Ueda, H., Nakagaki, M., Effect of water on lamellar structure of DPPC-sugar systems (1997) Biochim. Biophys. Acta, 1328, pp. 197-206
  • Pezron, E., Leibler, L., Lafuma, F., Complex formation in polymer-ion solutions. 2. Polyelectrolyte effect (1989) Macromolecules, 22, pp. 2656-2662
  • Pezron, E., Leibler, L., Ricard, A., Lafuma, F., Audebert, R., Complex formation in polymer-ion solutions. 2. Polymer concentration effects (1989) Macromolecules, 22, pp. 1169-1174
  • Rossi, S., Buera, M.P., Moreno, S., Chirife, J., Stabilization of the restriction enzyme EcoRI dried with trehalose and other selected glass-forming solutes (1997) Biotechnol. Prog., 13, pp. 609-616
  • Rumsey, S.C., Galeano, N.F., Arad, Y., Deckelbaum, R.J., Cryopreservation with sucrose maintains normal physical and biological properties of human plasma low density lipoproteins (1992) J. Lipid Res., 33, pp. 1551-1561
  • Schebor, C., Burin, L., Buera, M.P., Aguilera, J.M., Chirife, J., Glassy state and thermal inactivation of invertase and lactase in dried amorphous matrices (1997) Biotechnol. Prog., 13, pp. 857-863
  • Steponkus, P.L., Role of the plasma-membrane in freezing-injury and cold-acclimation (1984) Annu. Rev. Plant Physiol., 35, pp. 543-584
  • Sum, A.K., Faller, R., De Pablo, J.J., Molecular simulation study of phospholipid bilayers and insights of the interactions with disaccharides (2003) Biophys. J., 85, pp. 2830-2844
  • Sun, W.Q., Leopold, A.C., Crowe, L.M., Crowe, J.H., Stability of dry liposomes in sugar glasses (1996) Biophys. J., 70, pp. 1769-1776
  • Zhang, J., Steponkus, P.L., Proposed mechanism for depression of the liquid-crystalline-to-gel phase transition temperature of phospholipids in dehydrated sugar-phospholipid mixtures (1996) Cryobiology, 33, pp. 21A

Citas:

---------- APA ----------
Ohtake, S., Schebor, C., Palecek, S.P. & De Pablo, J.J. (2004) . Effect of sugar-phosphate mixtures on the stability of DPPC membranes in dehydrated systems. Cryobiology, 48(1), 81-89.
http://dx.doi.org/10.1016/j.cryobiol.2004.01.001
---------- CHICAGO ----------
Ohtake, S., Schebor, C., Palecek, S.P., De Pablo, J.J. "Effect of sugar-phosphate mixtures on the stability of DPPC membranes in dehydrated systems" . Cryobiology 48, no. 1 (2004) : 81-89.
http://dx.doi.org/10.1016/j.cryobiol.2004.01.001
---------- MLA ----------
Ohtake, S., Schebor, C., Palecek, S.P., De Pablo, J.J. "Effect of sugar-phosphate mixtures on the stability of DPPC membranes in dehydrated systems" . Cryobiology, vol. 48, no. 1, 2004, pp. 81-89.
http://dx.doi.org/10.1016/j.cryobiol.2004.01.001
---------- VANCOUVER ----------
Ohtake, S., Schebor, C., Palecek, S.P., De Pablo, J.J. Effect of sugar-phosphate mixtures on the stability of DPPC membranes in dehydrated systems. Cryobiology. 2004;48(1):81-89.
http://dx.doi.org/10.1016/j.cryobiol.2004.01.001