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Abstract:

We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximum at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent Km = 0.018 mM), was highest at 37°C but appeared to be cold- and heat-tolerant, since it was high at 4°C and at 45°C. Lipase activity was enhanced upon incubation of hepatopancreas with 10?4 M dopamine (about 100%), 2 × 10?3 M glucagon (about 250%) and 10?4 M cAMP (about 150%) suggesting a role of these chemical messengers in mechanisms of regulation of lipolytic activities and its direct effect on the hepatopancreas. The concomitant decrease in triglycerides content upon dopamine and cAMP treatment suggests a link between enhanced lipase activity by these messengers and triglycerides catabolism. © 2015 Koninklijke Brill NV, Leiden.

Registro:

Documento: Artículo
Título:Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
Autor:López Mañanes, A.A.; Del Valle, J.C.; Michiels, M.S.
Filiación:Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar Del Plata (UNMDP), Mar del Plata, Argentina
CONICET, Buenos Aires, Argentina
Palabras clave:burrowing organism; catabolism; crab; emergence; enzyme activity; identification key; incubation; temperature effect; Decapoda (Crustacea); Grapsidae
Año:2015
Volumen:88
Número:1
Página de inicio:51
Página de fin:65
DOI: http://dx.doi.org/10.1163/15685403-00003399
Título revista:Crustaceana
Título revista abreviado:Crustaceana
ISSN:0011216X
CODEN:CRUSA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0011216X_v88_n1_p51_LopezMananes

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Citas:

---------- APA ----------
López Mañanes, A.A., Del Valle, J.C. & Michiels, M.S. (2015) . Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae). Crustaceana, 88(1), 51-65.
http://dx.doi.org/10.1163/15685403-00003399
---------- CHICAGO ----------
López Mañanes, A.A., Del Valle, J.C., Michiels, M.S. "Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)" . Crustaceana 88, no. 1 (2015) : 51-65.
http://dx.doi.org/10.1163/15685403-00003399
---------- MLA ----------
López Mañanes, A.A., Del Valle, J.C., Michiels, M.S. "Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)" . Crustaceana, vol. 88, no. 1, 2015, pp. 51-65.
http://dx.doi.org/10.1163/15685403-00003399
---------- VANCOUVER ----------
López Mañanes, A.A., Del Valle, J.C., Michiels, M.S. Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae). Crustaceana. 2015;88(1):51-65.
http://dx.doi.org/10.1163/15685403-00003399