Artículo
Abstract:
We recorded fluorescence excitation and emission spectra of uro- and coproporphyrin under different experimental conditions, to see how these conditions influence quantification based on measurement of fluorescence intensity. We found that, for bands α and β of the emission spectra and the main peak of the excitation spectra, fluorescence depends on pH and is minimal near pH 5 and near pH 7-7.5 for copro- and uroporphyrin, respectively. For band γ of the emission spectra there was a constant decrease of fluorescence with increasing alkalinity of the solution. The intensity of porphyrin fluorescence also depends on ionic strength, reaching sharp maxima at 0.1 mol/L (for uroporphyrin) and 1 mol/L (for coproporphyrin). The organic mixture ethyl acetate:acetic acid (4:1 by vol), commonly used to extract porphyrins from biological samples, markedly diminishes the fluorescence of both porphyrins as compared with the same concentration of each porphyrin in aqueous acidic solvent. Furthermore, when we measured different ratios of uro:copro mixture at three distinct pHs and buffers, we found that at pH 10.5 (in carbonate buffer) the measured units of fluorescence depend only on total porphyrin concentration and not on the composition of the mixture.
Registro:
| Documento: | Artículo |
| Título: | Factors influencing fluorescence spectra of free porphyrins |
| Autor: | Polo, C.F.; Frisardi, A.L.; Resnik, E.R.; Schoua, A.E.M.; Del Batlle, C.A.M. |
| Filiación: | Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Ciudad Universitaria, 1428 Buenos Aires, Argentina |
| Palabras clave: | porphyrin; fluorescence; nonhuman; Coproporphyrins; Hydrogen-Ion Concentration; Osmolar Concentration; Porphyrins; Solvents; Spectrometry, Fluorescence; Support, Non-U.S. Gov't; Uroporphyrins |
| Año: | 1988 |
| Volumen: | 34 |
| Número: | 4 |
| Página de inicio: | 757 |
| Página de fin: | 760 |
| Título revista: | Clinical Chemistry |
| Título revista abreviado: | CLIN. CHEM. |
| ISSN: | 00099147 |
| CODEN: | CLCHA |
| CAS: | porphyrin, 24869-67-8; Coproporphyrins; Porphyrins; Solvents; Uroporphyrins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00099147_v34_n4_p757_Polo |
Citas:
---------- APA ----------
Polo, C.F., Frisardi, A.L., Resnik, E.R., Schoua, A.E.M. & Del Batlle, C.A.M. (1988) . Factors influencing fluorescence spectra of free porphyrins. Clinical Chemistry, 34(4), 757-760.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00099147_v34_n4_p757_Polo [ ]
---------- CHICAGO ----------
Polo, C.F., Frisardi, A.L., Resnik, E.R., Schoua, A.E.M., Del Batlle, C.A.M. "Factors influencing fluorescence spectra of free porphyrins" . Clinical Chemistry 34, no. 4 (1988) : 757-760.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00099147_v34_n4_p757_Polo [ ]
---------- MLA ----------
Polo, C.F., Frisardi, A.L., Resnik, E.R., Schoua, A.E.M., Del Batlle, C.A.M. "Factors influencing fluorescence spectra of free porphyrins" . Clinical Chemistry, vol. 34, no. 4, 1988, pp. 757-760.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00099147_v34_n4_p757_Polo [ ]
---------- VANCOUVER ----------
Polo, C.F., Frisardi, A.L., Resnik, E.R., Schoua, A.E.M., Del Batlle, C.A.M. Factors influencing fluorescence spectra of free porphyrins. CLIN. CHEM. 1988;34(4):757-760.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00099147_v34_n4_p757_Polo [ ]
