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Abstract:

BALB/c mice immunized with recombinant Trypanosoma cruzi ribosomal P2β protein (TcP2β) develop a strong and specific antibody response against its 13 residue-long C-terminal epitope (peptide R13: EEEDDDMGFGLFD) that has a concomitant β1-adrenergic stimulating activity. However, other animals that undergo similar immunizations seem tolerant to this epitope. To evaluate further the antibody response against the ribosomal P proteins, 25 BALB/c and 25 Swiss mice were immunized with TcP2β. From the 50 animals, 31 developed a positive anti-R13 response, whereas 19 were non-responsive. From the 31 anti-R13 positive mice, 25 had anti-R13 antibodies that recognized the discontinuous motif ExDDxGF, and their presence correlated with the recording of supraventricular tachycardia. The other six had anti-R13 antibodies but with a normal electrocardiographic recording. These anti-R13 antibodies recognized the motif DDxGF shared by mammals and T. cruzi and proved to be a true anti-P autoantibody because they were similar to those elicited in Swiss, but not in BALB/c mice, by immunization with the C-terminal portion of the mouse ribosomal P protein. Our results show that the recognition of the glutamic acid in position 3 of peptide R13 defines the ability of anti-R13 antibodies to react with the motif AESDE of the second extracellular loop of the β1-adrenergic receptor, setting the molecular basis for their pathogenic β1 adrenoceptor stimulating activity. © 2005 British Society for Immunology.

Registro:

Documento: Artículo
Título:The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition
Autor:Bergami, P.L.; Gómez, K.A.; Levy, G.V.; Grippo, V.; Baldi, A.; Levin, M.J.
Filiación:Laboratorio de Biologín Molecular de la Enfermedad de Chagas, Inst. Invest. en Ing. Genet./Biol. Molec.-Consejo Nac. de Invest. Cie./Tec. (INGEBI/CONICET)/CeGA, Centro de Genómica Aplicada-Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental (IBYME), Buenos Aires, Argentina
Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI), Vuelta de Obligado 2490, 1428, Buenos Aires, Argentina
Palabras clave:β1-adrenergic receptor; Pathogenic antibodies; Ribosomal P proteins; Trypanosoma cruzi; beta 1 adrenergic receptor; epitope; glutamylglutamylglutamylaspartylaspartylaspartylmethionylglycylphenylalanylglycylleucylphenylalanylaspartic acid; peptide; protein antibody; protein TcP2beta; protozoal protein; ribosome protein; unclassified drug; autoantibody; beta 1 adrenergic receptor; epitope; immunoglobulin G; protozoon antibody; recombinant protein; ribosome protein; adrenergic activity; amino acid sequence; animal experiment; animal model; antigen recognition; article; carboxy terminal sequence; controlled study; electrocardiography; epitope mapping; immune response; immunization; immunological tolerance; mouse; nonhuman; priority journal; protein analysis; protein motif; supraventricular tachycardia; Trypanosoma cruzi; animal; autoimmunity; Bagg albino mouse; Chagas disease; immunology; methodology; pathophysiology; Trypanosoma cruzi; Animals; Antibodies, Protozoan; Autoantibodies; Autoimmunity; Chagas Disease; Electrocardiography; Epitope Mapping; Epitopes; Immunoglobulin G; Mice; Mice, Inbred BALB C; Protozoan Proteins; Receptors, Adrenergic, beta-1; Recombinant Proteins; Ribosomal Proteins; Trypanosoma cruzi
Año:2005
Volumen:142
Número:1
Página de inicio:140
Página de fin:147
DOI: http://dx.doi.org/10.1111/j.1365-2249.2005.02885.x
Título revista:Clinical and Experimental Immunology
Título revista abreviado:Clin. Exp. Immunol.
ISSN:00099104
CODEN:CEXIA
CAS:immunoglobulin G, 97794-27-9; Antibodies, Protozoan; Autoantibodies; Epitopes; Immunoglobulin G; Protozoan Proteins; Receptors, Adrenergic, beta-1; Recombinant Proteins; Ribosomal Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00099104_v142_n1_p140_Bergami

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Citas:

---------- APA ----------
Bergami, P.L., Gómez, K.A., Levy, G.V., Grippo, V., Baldi, A. & Levin, M.J. (2005) . The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition. Clinical and Experimental Immunology, 142(1), 140-147.
http://dx.doi.org/10.1111/j.1365-2249.2005.02885.x
---------- CHICAGO ----------
Bergami, P.L., Gómez, K.A., Levy, G.V., Grippo, V., Baldi, A., Levin, M.J. "The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition" . Clinical and Experimental Immunology 142, no. 1 (2005) : 140-147.
http://dx.doi.org/10.1111/j.1365-2249.2005.02885.x
---------- MLA ----------
Bergami, P.L., Gómez, K.A., Levy, G.V., Grippo, V., Baldi, A., Levin, M.J. "The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition" . Clinical and Experimental Immunology, vol. 142, no. 1, 2005, pp. 140-147.
http://dx.doi.org/10.1111/j.1365-2249.2005.02885.x
---------- VANCOUVER ----------
Bergami, P.L., Gómez, K.A., Levy, G.V., Grippo, V., Baldi, A., Levin, M.J. The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition. Clin. Exp. Immunol. 2005;142(1):140-147.
http://dx.doi.org/10.1111/j.1365-2249.2005.02885.x