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Background: The diagnosis of thyroid follicular carcinoma by fine needle aspiration biopsy is a well known problem in thyroid pathology. Methods: We evaluated telomerase activity (TA) in 85 fine needle aspiration biopsy (FNAB) samples from patients with thyroid nodules. Surgery samples from patients with tumor or follicular adenomas were also analyzed. Results: Twenty of the FNAB samples corresponded to carcinomas and were positive to telomerase assay (TA > 10 Units). Among them, 4 follicular carcinomas and 1 papillary carcinoma were labeled as indeterminate by FNAB cytological examination. Four percent false positive cases and no false negative cases for TA in FNABs were reported. FNAB samples from follicular adenomas were diagnosed as indeterminate by cytological examination, but they showed no detectable TA. Tumor tissues from patients with follicular or papillary thyroid carcinomas presented TA > 10 Units, whereas follicular adenoma tissues (benign nodules) showed no TA. Conclusion: Our results showed a good correlation between TA in FNAB samples and tumor/nodule thyroid tissue. This suggested that use of TA as a biological marker of malignancy might be a useful tool in the diagnosis of follicular thyroid carcinomas or follicular thyroid adenomas using FNAB samples. © 2006 Elsevier B.V. All rights reserved.


Documento: Artículo
Título:Telomerase activity in fine needle aspiration biopsy samples: Application to diagnosis of human thyroid carcinoma
Autor:Guerra, L.N.; Miler, E.A.; Moiguer, S.; Karner, M.; Orlandi, A.M.; Fideleff, H.; Burdman, J.A.
Filiación:Endocrinology Unit, Hospital Israelita EZRAH, Buenos Aires, Argentina
ORT Argentina, Buenos Aires, Argentina
Endocrinology Unit, Hospital Municipal T. Alvarez, Buenos Aires, Argentina
Department of Biological Chemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 4o piso, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Fine needle aspiration biopsy; Telomerase; Thyroid cancer; telomerase; article; aspiration biopsy; cancer cytodiagnosis; cell labeling; controlled study; cytopathology; enzyme activity; false negative result; false positive result; histopathology; human; human tissue; major clinical study; needle biopsy; papillary carcinoma; priority journal; sample size; thyroid carcinoma; thyroid follicular carcinoma; thyroid nodule; tissue section; Biopsy, Fine-Needle; Humans; Telomerase; Thyroid Neoplasms; Tumor Markers, Biological; Tumor Suppressor Protein p53
Página de inicio:180
Página de fin:184
Título revista:Clinica Chimica Acta
Título revista abreviado:Clin. Chim. Acta
CAS:Telomerase, EC; Tumor Markers, Biological; Tumor Suppressor Protein p53


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---------- APA ----------
Guerra, L.N., Miler, E.A., Moiguer, S., Karner, M., Orlandi, A.M., Fideleff, H. & Burdman, J.A. (2006) . Telomerase activity in fine needle aspiration biopsy samples: Application to diagnosis of human thyroid carcinoma. Clinica Chimica Acta, 370(1-2), 180-184.
---------- CHICAGO ----------
Guerra, L.N., Miler, E.A., Moiguer, S., Karner, M., Orlandi, A.M., Fideleff, H., et al. "Telomerase activity in fine needle aspiration biopsy samples: Application to diagnosis of human thyroid carcinoma" . Clinica Chimica Acta 370, no. 1-2 (2006) : 180-184.
---------- MLA ----------
Guerra, L.N., Miler, E.A., Moiguer, S., Karner, M., Orlandi, A.M., Fideleff, H., et al. "Telomerase activity in fine needle aspiration biopsy samples: Application to diagnosis of human thyroid carcinoma" . Clinica Chimica Acta, vol. 370, no. 1-2, 2006, pp. 180-184.
---------- VANCOUVER ----------
Guerra, L.N., Miler, E.A., Moiguer, S., Karner, M., Orlandi, A.M., Fideleff, H., et al. Telomerase activity in fine needle aspiration biopsy samples: Application to diagnosis of human thyroid carcinoma. Clin. Chim. Acta. 2006;370(1-2):180-184.