Artículo

Serra, J.A.; Marschoff, E.R.; Domínguez, R.O.; De Lustig, E.S.; Famulari, A.L.; Bartolomé, E.L.; Guareschi, E.M. "Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures" (2000) Clinica Chimica Acta. 301(1-2):87-102
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Abstract:

As oxidative stress in relation with neurological diseases has become an important point in recent research, simple methods to be used in epidemiological studies and clinical practice are required. The hypothesis that the analytical methods used in research laboratories (RLM) can be used interchangeably with commercial kits (CKM) for SOD and TRAP is tested. Both methods were compared using linear transformations of the RLM measurements into the CKM scales. Data were obtained from Alzheimer's, Parkinson's, and vascular dementia patients and controls. The lack of fit and the run's test of residuals were not significant, but the same sign method detected significant nonlinearities (P < 0.000001 for SOD, P < 0.01 for TRAP). The intragroup CVs of both methods were comparable for TRAP, while in the RLM determinations of SOD resulted in <50% of those obtained with the CKM. The ANCOVA comparison of the regression parameters across the clinical groups resulted significant for SOD (P < 0.0001) and not significant for TRAP. Both methods agree in describing the features of the clinical groups, but the degree of agreement at the individual concentration was poor and they could not be readily intercalibrated. Normal and pathological values should be obtained independently for the CKM to insure their applicability to large populations. (C) 2000 Elsevier Science B.V.

Registro:

Documento: Artículo
Título:Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures
Autor:Serra, J.A.; Marschoff, E.R.; Domínguez, R.O.; De Lustig, E.S.; Famulari, A.L.; Bartolomé, E.L.; Guareschi, E.M.
Filiación:Consejo Nac. Invest. Cie. y Tec. (C., Buenos Aires, Argentina
Fac. de Ciencias Exactas y Naturales, UBA, Buenos Aires, Argentina
Hospital Sirio-Libanés, FACENE, Buenos Aires, Argentina
Inst. de Oncol. Angel H. Roffo, UBA, Buenos Aires, Argentina
Lab. Especializado las Invest. C., Buenos Aires, Argentina
Palabras clave:Analytical methods; Antioxidant capacity; Neurological diseases; Oxidative stress; Superoxide dismutase; antioxidant; superoxide dismutase; Alzheimer disease; analytic method; article; clinical practice; controlled study; correlation function; disease marker; female; human; major clinical study; male; medical research; multiinfarct dementia; neurologic disease; oxidative stress; Parkinson disease; patient selection; priority journal; Alzheimer Disease; Antioxidants; Case-Control Studies; Comparative Study; Dementia, Vascular; Human; Parkinson Disease; Superoxide Dismutase; Support, Non-U.S. Gov't
Año:2000
Volumen:301
Número:1-2
Página de inicio:87
Página de fin:102
DOI: http://dx.doi.org/10.1016/S0009-8981(00)00326-0
Título revista:Clinica Chimica Acta
Título revista abreviado:Clin. Chim. Acta
ISSN:00098981
CODEN:CCATA
CAS:superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Antioxidants; Superoxide Dismutase, EC 1.15.1.1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00098981_v301_n1-2_p87_Serra

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Citas:

---------- APA ----------
Serra, J.A., Marschoff, E.R., Domínguez, R.O., De Lustig, E.S., Famulari, A.L., Bartolomé, E.L. & Guareschi, E.M. (2000) . Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures. Clinica Chimica Acta, 301(1-2), 87-102.
http://dx.doi.org/10.1016/S0009-8981(00)00326-0
---------- CHICAGO ----------
Serra, J.A., Marschoff, E.R., Domínguez, R.O., De Lustig, E.S., Famulari, A.L., Bartolomé, E.L., et al. "Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures" . Clinica Chimica Acta 301, no. 1-2 (2000) : 87-102.
http://dx.doi.org/10.1016/S0009-8981(00)00326-0
---------- MLA ----------
Serra, J.A., Marschoff, E.R., Domínguez, R.O., De Lustig, E.S., Famulari, A.L., Bartolomé, E.L., et al. "Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures" . Clinica Chimica Acta, vol. 301, no. 1-2, 2000, pp. 87-102.
http://dx.doi.org/10.1016/S0009-8981(00)00326-0
---------- VANCOUVER ----------
Serra, J.A., Marschoff, E.R., Domínguez, R.O., De Lustig, E.S., Famulari, A.L., Bartolomé, E.L., et al. Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures. Clin. Chim. Acta. 2000;301(1-2):87-102.
http://dx.doi.org/10.1016/S0009-8981(00)00326-0