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Abstract:

Background: Dehydroepiandrosterone (DHEA) exhibits a wide range of biological functions including antiviral activity. In this work, we present in vitro anti-adenovirus (AdV) activity of seven DHEA and twelve epiandrosterone (EA) analogues. Methods: The cytotoxic effect of the compounds was determined by the MTT assay and the antiviral activity by a virus yield inhibition assay. The mode of antiviral activity was examined using time-of-addition experiments, adsorption and internalization assays and Western blot analysis. Results: EA, DHEA, and two synthetic derivatives inhibit virus replication with selectivity indices ranging between 42 and 83. Virus adsorption and internalization are not the target of the inhibitory action; meanwhile, AdV protein synthesis was diminished in the presence of DHEA. Conclusions: DHEA and some synthetic derivatives present antiviral activity similar to cidofovir, which was used as reference drug. These steroidal compounds adversely affect virus protein synthesis and viral mature particle formation. Copyright © 2010 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives
Autor:Romanutti, C.; Bruttomesso, A.C.; Castilla, V.; Galagovsky, L.R.; Wachsman, M.B.
Filiación:Laboratorio de Virología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria Pabellón 2, Piso 4, C1428EGA, Buenos Aires, Argentina
Departamento de Química Orgánica y UMYMFOR, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Adenovirus; Anti-adenovirus activity; Antiviral activity; Dehydroepiandrosterone analogues; DHEA; 16alpha hydroxyprasterone; 17 oxo 5alpha androstan 3beta yl acetate; 17 oxo 5alpha androstan 3beta,16alpha diyl diacetate; 17 oxo 5alpha androstan 3beta,16beta diyl diacetate; 17 oxoandrost 5 en 3beta yl acetate; 17 oxoandrost 5 en 3beta,16alpha diyl diacetate; 17,17 ethylendioxy 5alpha androst 15 en 3beta ol; 17,17 ethylendioxy 5alpha androstan 16alpha bromo 3beta ol; 17,17 ethylendioxy 5alpha androstan 3beta ol; 17,17 ethylendioxy 5alpha androstan 3beta yl acetate; 17,17 ethylendioxyandrost 5 en 3beta ol; 21 norpregna 5,17(20) dien 3beta,16alpha diyl diacetate; 3beta hydroxy 5alpha androst 15 en 17 one; 3beta,15alpha dihydroxy 5alpha androstan 17 one; 3beta,15beta dihydroxy 5alpha androstan 17 one; 3beta,16alpha dihydroxy 5alpha androstan 17 one; 3beta,6alpha dihydroxy 5alpha androstan 17 one; androsta 5,16 dien 3beta,17 diyl diacetate; antivirus agent; cidofovir; epiandrosterone; prasterone; unclassified drug; virus protein; Adenovirus; antiviral activity; article; controlled study; drug cytotoxicity; drug mechanism; drug selectivity; drug structure; human; human cell; in vitro study; priority journal; protein synthesis regulation; virus attachment; virus entry; virus inhibition; virus particle; virus replication; Western blotting; Adenoviridae; Androsterone; Animals; Antiviral Agents; Blotting, Western; Cercopithecus aethiops; Cytosine; Dehydroepiandrosterone; Humans; Mice; Phosphonic Acids; Vero Cells; Viral Proteins; Virus Replication
Año:2010
Volumen:56
Número:2
Página de inicio:158
Página de fin:165
DOI: http://dx.doi.org/10.1159/000313530
Título revista:Chemotherapy
Título revista abreviado:Chemotherapy
ISSN:00093157
CODEN:CHTHB
CAS:cidofovir, 113852-37-2; epiandrosterone, 481-29-8; prasterone, 53-43-0; Androsterone, 53-41-8; Antiviral Agents; Cytosine, 71-30-7; Dehydroepiandrosterone, 53-43-0; Phosphonic Acids; Viral Proteins; cidofovir, 113852-37-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00093157_v56_n2_p158_Romanutti

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Citas:

---------- APA ----------
Romanutti, C., Bruttomesso, A.C., Castilla, V., Galagovsky, L.R. & Wachsman, M.B. (2010) . Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives. Chemotherapy, 56(2), 158-165.
http://dx.doi.org/10.1159/000313530
---------- CHICAGO ----------
Romanutti, C., Bruttomesso, A.C., Castilla, V., Galagovsky, L.R., Wachsman, M.B. "Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives" . Chemotherapy 56, no. 2 (2010) : 158-165.
http://dx.doi.org/10.1159/000313530
---------- MLA ----------
Romanutti, C., Bruttomesso, A.C., Castilla, V., Galagovsky, L.R., Wachsman, M.B. "Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives" . Chemotherapy, vol. 56, no. 2, 2010, pp. 158-165.
http://dx.doi.org/10.1159/000313530
---------- VANCOUVER ----------
Romanutti, C., Bruttomesso, A.C., Castilla, V., Galagovsky, L.R., Wachsman, M.B. Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives. Chemotherapy. 2010;56(2):158-165.
http://dx.doi.org/10.1159/000313530