Abstract:
The permeability of liposomes to water, glucose, Ca2+ and alkaline cations was monitored by recording the change in absorbance at 450 nm using a rapid reaction stopped-flow spectrophotometer. Liposomes were prepared with egg phosphatidylcholine and concentrations of dolichol ranging from 0.1% to 9% (w/w). Net permeability of phosphatidylcholine bilayers to alkaline cations was induced by the incorporation of dolichol. This effect was not observed in the case of non-charged solutes like glucose or in that of alkaline earth cations such as calcium. Permeation of K+ was significantly increased above the phase transition temperature. These results suggest that dolichols may play a role in biological membranes, besides the well-known glycosyl carrier function in the biosynthesis of glycoproteins. © 1985.
Registro:
Documento: |
Artículo
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Título: | The effect of dolichol on the permeability properties of phosphatidylcholine bilayers |
Autor: | Boscoboinik, D.O.; Feliz, M.; Disalvo, E.A.; Belocopitow, E. |
Filiación: | Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, CONICET, Antonio Machado 151, 1405 Buenos Aires, Argentina Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas (INIFTA INIFTA is a research Inst., Casilla de Correo 16, Sucursal 4, 1900 La Plata, Argentina
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Palabras clave: | dolichol; permeability; phosphatidylcholine bilayers; dolichol; phosphatidylcholine; membrane permeability; nonbiological model; nonhuman; phospholipid bilayer; priority journal; theoretical study; Calcium; Diterpenes; Dolichol; Glucose; Lipid Bilayers; Liposomes; Permeability; Phosphatidylcholines; Potassium; Temperature |
Año: | 1985
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Volumen: | 38
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Número: | 4
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Página de inicio: | 343
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Página de fin: | 352
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DOI: |
http://dx.doi.org/10.1016/0009-3084(85)90028-3 |
Título revista: | Chemistry and Physics of Lipids
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Título revista abreviado: | Chem. Phys. Lipids
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ISSN: | 00093084
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CODEN: | CPLIA
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CAS: | dolichol, 11029-02-0; phosphatidylcholine, 55128-59-1, 8002-43-5; Calcium, 7440-70-2; Diterpenes; Dolichol, 2067-66-5; Glucose, 50-99-7; Lipid Bilayers; Liposomes; Phosphatidylcholines; Potassium, 7440-09-7
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00093084_v38_n4_p343_Boscoboinik |
Referencias:
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Citas:
---------- APA ----------
Boscoboinik, D.O., Feliz, M., Disalvo, E.A. & Belocopitow, E.
(1985)
. The effect of dolichol on the permeability properties of phosphatidylcholine bilayers. Chemistry and Physics of Lipids, 38(4), 343-352.
http://dx.doi.org/10.1016/0009-3084(85)90028-3---------- CHICAGO ----------
Boscoboinik, D.O., Feliz, M., Disalvo, E.A., Belocopitow, E.
"The effect of dolichol on the permeability properties of phosphatidylcholine bilayers"
. Chemistry and Physics of Lipids 38, no. 4
(1985) : 343-352.
http://dx.doi.org/10.1016/0009-3084(85)90028-3---------- MLA ----------
Boscoboinik, D.O., Feliz, M., Disalvo, E.A., Belocopitow, E.
"The effect of dolichol on the permeability properties of phosphatidylcholine bilayers"
. Chemistry and Physics of Lipids, vol. 38, no. 4, 1985, pp. 343-352.
http://dx.doi.org/10.1016/0009-3084(85)90028-3---------- VANCOUVER ----------
Boscoboinik, D.O., Feliz, M., Disalvo, E.A., Belocopitow, E. The effect of dolichol on the permeability properties of phosphatidylcholine bilayers. Chem. Phys. Lipids. 1985;38(4):343-352.
http://dx.doi.org/10.1016/0009-3084(85)90028-3