Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Monolayers spread on Hg drops are shown as a suitable experimental set up to study the influence of external electric fields on the structure of lipid membranes. The electrical response exhibits a sharp transition at 24°C, the transition temperature of DMPC. In addition, voltammetric response of monolayers of mixtures of DMPC/DMPE adsorbed on mercury, shows a similar trend to that found for dipole potential of monolayers of the same composition spread on an air-solution interface. It is concluded that a lipid monolayer adsorbed in a mercury-solution interface, has comparable properties as those found in other experimental models of lipid membranes in similar conditions. In addition, they constitute an ideal set up to study the effect of electrical fields on the dynamic conformation of lipids as a function of packing change produced by the condensation in the gel state or by the interaction of polar head groups. © 2005 Elsevier Ireland Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Lipid monolayers on Hg as a valid experimental model for lipid membranes under electrical fields
Autor:Almaleck, S.H.; Lairion, F.; Disalvo, E.A.; Gordillo, G.J.
Filiación:Departamento de Química Analítica Y Fisicoquímica, Cátedra de Química General e Inorgánica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina
Departamento de Química Inorgánica, Analítica Y Química Física, Facultad de Ciencias Exactas Y Naturales, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Cyclic voltammetry; Electrical fields; Hanging drop mercury electrode; Lipid membranes; Lipid monolayers; dimirystoylphosphatidylethanolamine; dimyristoylphosphatidylcholine; ethanolamine derivative; mercury; unclassified drug; adsorption; article; bilayer membrane; cyclic potentiometry; dipole; electric activity; electric field; experimental model; lipid membrane; lipid monolayer; membrane structure; priority journal; transition temperature; Chemistry, Physical; Electrochemistry; Electromagnetic Fields; Lipids; Membranes, Artificial; Mercury; Temperature
Año:2006
Volumen:139
Número:2
Página de inicio:150
Página de fin:156
DOI: http://dx.doi.org/10.1016/j.chemphyslip.2005.12.001
Título revista:Chemistry and Physics of Lipids
Título revista abreviado:Chem. Phys. Lipids
ISSN:00093084
CODEN:CPLIA
CAS:dimyristoylphosphatidylcholine, 13699-48-4, 18194-24-6; mercury, 14302-87-5, 7439-97-6; Lipids; Membranes, Artificial; Mercury, 7439-97-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00093084_v139_n2_p150_Almaleck

Referencias:

  • Becucci, L., Moncelli, M.R., Guidelli, R., Thallous ion movements trough gramicidin channels incorporated in lipid monolayers supported by mercury (2002) Biophys. J., 82 (2), pp. 852-864
  • Bizzotto, D., Nelson, A., Continuing electrochemical studies of phospholipid monolayers of dioleoyl phosphatidylcholine at the mercury-electrolite interface (1998) Langmuir, 14, pp. 6269-6273
  • Blume, A., A comparative study of the phase transitions of phospholipid bilayers and monolayers (1979) Biochim. Biophys. Acta - Biomembr., 557, pp. 32-44
  • Boggs, J.M., Lipid intermolecular hydrogen bonding: Influence on structural organization and membrane function (1987) Biochim. Biophys. Acta, 906, pp. 353-404
  • Coster, H.G.L., Chilcott, T.C., The characterization of membranes and membrane surfaces using impedance spectroscopy (1999) Surface Chemistry and Electrochemistry of Membranes, Surface Science Series, 7919, pp. 749-792. , T.S. Sorensen Marcel Dekker, Inc. New York
  • Diaz, S., Amalfa, F., Biondi De Lopez, A.A., Disalvo, E.A., Effect of water polarized at the carbonyl groups of phosphatidylcholines on the dipole potential of lipid bilayers (1999) Langmuir, 15 (15), pp. 5179-5182
  • Franklin, J.C., Cafiso, D.S., Internal electrostatic potentials in bilayers: Measuring and controlling dipole potentials in lipid vesicles (1993) Biophys. J., 65, pp. 289-299
  • Gordillo, G.J., Schiffrin, D.J., The electrochemistry of ubiquinone-10 in a phospholipìd model membrane (2000) Faraday Discuss., 116, pp. 89-107
  • Lecompte, M.F., Miller, I.R., Interaction of prothrombin and its fragments with monolayers containing phosphatidylserine. 2. Electrochemical determination of lipid layer perturbation by interacting prothrombin and its fragments (1980) Biochemistry, 19 (15), pp. 3439-3446
  • Leermakers, F.A.M., Nelson, A., Substrate-induced structural changes in electrode-adsorbed lipid layers: A self-consistent field theory (1990) J. Electroanal. Chem., 278, pp. 53-72
  • Lundkvist, M., Molecular arrangement in condensed monolayers phases (1978) Progr. Chem. Fats Other Lipids, 16, pp. 101-124
  • Luzardo Del M, C., Peltzer, G., Disalvo, E.A., Surface potential of lipid interfaces formed by mixtures of phosphatidylcholine of different chain lengths (1998) Langmuir, 14 (20), pp. 5858-5862
  • Mac Donald, R.C., Simon, S.A., Lipid monolayer states and their relationships to bilayers (1987) Proc. Natl. Acad. Sci. U.S.A., 84, pp. 4089-4093
  • Marsh, D., (1990) Handbook of Lipid Bilayers, , CRC Press
  • Miller, I.R., Doll, L., Lester, D.S., Interaction of alamethicin, melittin and protein kinase C with pure and phospholipid monolayer covered mercury electrode surfaces (1992) Bioelectrochem. Bioenerg., 28, pp. 85-103
  • Moncelli, M.R., Becucci, L., Guidelli, R., The intrinsic pKa values for phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine in monolayers deposited on mercury electrodes (1994) Biophys. J., 66, pp. 1969-1980
  • Moncelli, M.R., Becucci, L., Tadini Buoninsegni, F., Guidelli, R., Surface dipole potential at the interface between water and self-assembled monolayers of phosphatidylserine and phosphatidic acid (1998) Biophys. J., 74, pp. 2388-2397
  • Moncelli, M.R., Becucci, L., The intrinsic pKa values for phosphatidic acid in monolayers deposited on mercury electrodes (1995) J. Electroanal. Chem., 385, pp. 183-189
  • Mouritsen, O.G., Theoretical models of phospholipid phase transitions (1991) Topical Issue of Chem. Phys. Lipids, 57. , P. Kinnunen P. Laggner
  • Nelson, A., Auffret, T., Phospholipid monolayers of dioleoyl lecithin at the mercury/water interface (1988) J. Electroanal. Chem., 244, pp. 99-113
  • Nelson, A., Benton, A., Phospholipid monolayers at the mercury/water interface (1986) J. Electroanal. Chem., 202, pp. 253-270
  • Nelson, A., Leermakers, F.A.M., Substrate-induced structural changes in electrode-adsorbed lipid layers. Experimental evidence from the behaviour of phospholipid layers on the mercury-water interface (1990) J. Electroanal. Chem., 278, pp. 73-83
  • Phillips, M.C., Williams, R.M., Chapman, D., On the nature of hydrocarbon chain motions in lipid liquid crystals (1969) Chem. Phys. Lipids, 3, pp. 234-244
  • Seelig, J., Mac Donald, P.M., Scherer, P.G., Phospholipid head groups as sensors of electric charge in membranes (1987) Biochemistry, 26, pp. 7535-7541
  • Simon, S.A., Lis, L.J., Kauffman, J.W., MacDonald, R.C., A calorimetric and monolayer investigation of the influence of ions on the thermodynamic properties of phosphatidylcholine (1975) Biochim. Biophys. Acta - Biomembr., 375, pp. 317-326
  • Smaby, J.M., Brockman, H.L., Surface dipole moments of lipids at the argon-water interface. Similarities among glycerol-ester-based lipids (1990) Biophys. J., 58 (1), pp. 195-204

Citas:

---------- APA ----------
Almaleck, S.H., Lairion, F., Disalvo, E.A. & Gordillo, G.J. (2006) . Lipid monolayers on Hg as a valid experimental model for lipid membranes under electrical fields. Chemistry and Physics of Lipids, 139(2), 150-156.
http://dx.doi.org/10.1016/j.chemphyslip.2005.12.001
---------- CHICAGO ----------
Almaleck, S.H., Lairion, F., Disalvo, E.A., Gordillo, G.J. "Lipid monolayers on Hg as a valid experimental model for lipid membranes under electrical fields" . Chemistry and Physics of Lipids 139, no. 2 (2006) : 150-156.
http://dx.doi.org/10.1016/j.chemphyslip.2005.12.001
---------- MLA ----------
Almaleck, S.H., Lairion, F., Disalvo, E.A., Gordillo, G.J. "Lipid monolayers on Hg as a valid experimental model for lipid membranes under electrical fields" . Chemistry and Physics of Lipids, vol. 139, no. 2, 2006, pp. 150-156.
http://dx.doi.org/10.1016/j.chemphyslip.2005.12.001
---------- VANCOUVER ----------
Almaleck, S.H., Lairion, F., Disalvo, E.A., Gordillo, G.J. Lipid monolayers on Hg as a valid experimental model for lipid membranes under electrical fields. Chem. Phys. Lipids. 2006;139(2):150-156.
http://dx.doi.org/10.1016/j.chemphyslip.2005.12.001