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Abstract:

In the current study, we analyze the immunomodulatory effect of oral transgene administration of IL-10 using a mice model of viral inflammation. Salmonella harboring a plasmid encoding the IL-10 gene (SLIL10) was administered by the oral route together with Salmonella carrying a plasmid encoding the glycoprotein D or B (SLgD, SLgB) of Herpes simplex virus type 2 (HSV-2). This resulted in a high inhibition of the cellular and human immune response against the viral proteins. When mice immunized against the HSV proteins were challenged with 10 lethal doses of HSV-2 by the intravaginal route, only those that had also received SLIL10 showed severe lesions and died. When Salmonella harboring pIL10 was administered orally to mice immunized by the intramuscular route with a plasmid encoding gD, inhibition of cellular and humoral immune responses were also observed but to a lesser extent than with oral immunization. By means of adoptive transfer experiments and in vitro experiments, we have subsequently determined that the mechanism possibly involved in the inhibition of the immune response could be a reduced antigenic presentation when mice receive SLIL10 that induced a state of anergy on specific T lymphocytes. © 2002 Elsevier Science (USA). All rights reserved.

Registro:

Documento: Artículo
Título:Modulation of the immune response mediated by oral transgene administration of IL-10
Autor:Elías, F.; Fló, J.
Filiación:Faculty of Exact and Natural Sciences, University of Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:DNA immunization; HSV; IL-10; Immune suppression; Salmonella; gamma interferon; glycoprotein B; glycoprotein D; interleukin 10; interleukin 2; virus antibody; virus protein; antigen presentation; article; bacterial strain; cellular immunity; controlled study; disease model; drug effect; female; genetic code; Herpes simplex virus 2; humoral immunity; immunization; immunomodulation; lymphocyte proliferation; mouse; nonhuman; plasmid; priority journal; protein synthesis; Salmonella; transgene; vaginitis; virus infection; Administration, Oral; Animals; Antibody Formation; Disease Models, Animal; Down-Regulation; Female; Herpes Genitalis; Herpes Simplex; Herpesvirus 2, Human; Immunity, Cellular; Injections, Intramuscular; Interleukin-10; Mice; Mice, Inbred BALB C; Plasmids; Salmonella; Transformation, Bacterial; Transgenes; Vaccination; Vaccines, Synthetic; Viral Envelope Proteins; Human herpesvirus 2; Salmonella; Simplexvirus
Año:2002
Volumen:216
Número:1-2
Página de inicio:73
Página de fin:81
DOI: http://dx.doi.org/10.1016/S0008-8749(02)00507-5
Título revista:Cellular Immunology
Título revista abreviado:Cell. Immunol.
ISSN:00088749
CODEN:CLIMB
CAS:glycoprotein B, herpes simplex virus type 2; glycoprotein D-herpes simplex virus type 2; Interleukin-10, 130068-27-8; Vaccines, Synthetic; Viral Envelope Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00088749_v216_n1-2_p73_Elias

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Citas:

---------- APA ----------
Elías, F. & Fló, J. (2002) . Modulation of the immune response mediated by oral transgene administration of IL-10. Cellular Immunology, 216(1-2), 73-81.
http://dx.doi.org/10.1016/S0008-8749(02)00507-5
---------- CHICAGO ----------
Elías, F., Fló, J. "Modulation of the immune response mediated by oral transgene administration of IL-10" . Cellular Immunology 216, no. 1-2 (2002) : 73-81.
http://dx.doi.org/10.1016/S0008-8749(02)00507-5
---------- MLA ----------
Elías, F., Fló, J. "Modulation of the immune response mediated by oral transgene administration of IL-10" . Cellular Immunology, vol. 216, no. 1-2, 2002, pp. 73-81.
http://dx.doi.org/10.1016/S0008-8749(02)00507-5
---------- VANCOUVER ----------
Elías, F., Fló, J. Modulation of the immune response mediated by oral transgene administration of IL-10. Cell. Immunol. 2002;216(1-2):73-81.
http://dx.doi.org/10.1016/S0008-8749(02)00507-5