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Abstract:

The nucleolar organizer activity and the presence of rDNA zones were studied in Tricepiro Don René INTA, a synthetic forage crop (2n=42,6x, AABBRR with introgression of Thinopyrum in 6A chromosome). The use of pTa71 probe revealed the presence of six rDNA zones but Ag-NOR indicated the presence of only four NORs (nucleolus organizer regions). Differential amphiplasty phenomenon could explain the inactivity of some rDNA zones. Simultaneous use of FISH (pTa71 probe) and DAPI banding analysis indicated that the chromosomes with rDNA zones belong to two rye and to four wheat chromosomes. The maximum number of nucleoli observed in somatic cells and the number of chromosomes with secondary constrictions agreed with the four active NORs detected with the Ag-staining method. The use of the pSc119.2 probe allowed to recognize that chromosomes with secondary constrictions are the wheat chromosomes IB and 6 B. The results obtained using classical and molecular cytogenetics lead us to conclude that this crop has a similar behavior to that observed in hexaploid triticales in relation to the phenomenon of amphiplasty. Furthermore, the complexity of genetic interactions between wheat and rye genomes described in triticales, are not modified by the presence of Thinopyron introgression in Tricepiro Don René INTA. © 2006 Taylor and Francis Group, LLC.

Registro:

Documento: Artículo
Título:Nucleolar organizer activity and competition in tricepiro don rené inta, a synthetic forage crop
Autor:Ferrari, M.R.; Fradkin, M.; Greizerstein, E.J.; Naranjo, C.A.; Poggio, L.
Filiación:CIGEN (CONICET-UNLP-CIC), Inst. Filotécnico de Sta Catalina (FCAF UNLP), C.C. 4, 1836 Llavallol, Bs. As, Argentina
Lab. de Citogenética y Evolución, Depto. Ecología, Genética y Evolución (FCEN, UBA), Buenos Aires, 1428, Argentina
Facultad de Ciencias Veterinarias, Área Física Biológica, Universidad de Buenos Aires, Chorroarín, 280 (1427), Argentina
Palabras clave:Ag-NORs; Amphiplasty; Nucleolar competition; PSc119.2; PTa71; Tricepiro; Secale cereale; Thinopyron; Thinopyrum; Triticosecale; Triticum aestivum
Año:2006
Volumen:59
Número:1
Página de inicio:47
Página de fin:52
DOI: http://dx.doi.org/10.1080/00087114.2006.10797898
Título revista:Caryologia
Título revista abreviado:Caryologia
ISSN:00087114
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00087114_v59_n1_p47_Ferrari

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Citas:

---------- APA ----------
Ferrari, M.R., Fradkin, M., Greizerstein, E.J., Naranjo, C.A. & Poggio, L. (2006) . Nucleolar organizer activity and competition in tricepiro don rené inta, a synthetic forage crop. Caryologia, 59(1), 47-52.
http://dx.doi.org/10.1080/00087114.2006.10797898
---------- CHICAGO ----------
Ferrari, M.R., Fradkin, M., Greizerstein, E.J., Naranjo, C.A., Poggio, L. "Nucleolar organizer activity and competition in tricepiro don rené inta, a synthetic forage crop" . Caryologia 59, no. 1 (2006) : 47-52.
http://dx.doi.org/10.1080/00087114.2006.10797898
---------- MLA ----------
Ferrari, M.R., Fradkin, M., Greizerstein, E.J., Naranjo, C.A., Poggio, L. "Nucleolar organizer activity and competition in tricepiro don rené inta, a synthetic forage crop" . Caryologia, vol. 59, no. 1, 2006, pp. 47-52.
http://dx.doi.org/10.1080/00087114.2006.10797898
---------- VANCOUVER ----------
Ferrari, M.R., Fradkin, M., Greizerstein, E.J., Naranjo, C.A., Poggio, L. Nucleolar organizer activity and competition in tricepiro don rené inta, a synthetic forage crop. Caryologia. 2006;59(1):47-52.
http://dx.doi.org/10.1080/00087114.2006.10797898