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Abstract:

Polysiphonia nigrescens was sequentially extracted with water at room temperature, 70 and 90 °C. The extracts were analyzed and the major one, isolated at 70 °C, was fractionated by ion-exchange chromatography, eluting with water and solutions of increasing sodium chloride concentration; five main fractions were separated. Structural analysis, carried out by methylation analysis and NMR spectroscopy, showed that four of these were partially cyclized agarans that were highly substituted on C-6 mainly with sulfate, although methyl ether and single stubs of β-d-xylose were found in minor proportions. A fifth fraction comprising 6-sulfated agarose was also isolated. The use of 2D NMR techniques allowed us to assign the 1H and 13C NMR resonances of the G6S→L6S diad for the first time. © 2008 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)
Autor:Prado, H.J.; Ciancia, M.; Matulewicz, M.C.
Filiación:Departamento de Química Orgánica, CIHIDECAR-CONICET, Facultad de Ciencias Exactas y Naturales, Pabellon 2, Ciudad Universitaria, 1428EGA Buenos Aires, Argentina
Cátedra de Química Orgánica, Departamento de Biología Aplicada y Alimentos (CIHIDECAR-CONICET), Facultad de Agronomía, Avenida San Martín 4453, 1417DSE Buenos Aires, Argentina
Palabras clave:Agarans; Algal polysaccharides; Polysiphonia nigrescens; Rhodomelaceae; Structural analysis; Sulfated galactans; Algal polysaccharides; Polysiphonia nigrescens; Rhodomelaceae; Sulfated galactans; Algae; Chromatographic analysis; Ion exchange; Methylation; Nuclear magnetic resonance spectroscopy; Sodium chloride; Polysaccharides; agaran; agarose; beta dextro xylose; dimethyl ether; hexamethonium bromide; polysaccharide; sodium chloride; sulfate; unclassified drug; water; xylose; article; carbon nuclear magnetic resonance; ceramiales; concentration (parameters); cyclization; drug isolation; elution; extraction; fractionation; ion exchange chromatography; methylation; nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; polysiphonia nigrescens; priority journal; proton nuclear magnetic resonance; rhodomelaceae; room temperature; seaweed; separation technique; structure analysis; Alkalies; Cell Extracts; Chromatography, Ion Exchange; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Polysaccharides; Seaweed; Temperature; algae; Ceramiales; Polysiphonia nigrescens; Rhodomelaceae
Año:2008
Volumen:343
Número:4
Página de inicio:711
Página de fin:718
DOI: http://dx.doi.org/10.1016/j.carres.2007.12.024
Título revista:Carbohydrate Research
Título revista abreviado:Carbohydr. Res.
ISSN:00086215
CODEN:CRBRA
CAS:agarose, 9012-36-6; dimethyl ether, 115-10-6; hexamethonium bromide, 55-97-0; sodium chloride, 7647-14-5; sulfate, 14808-79-8; water, 7732-18-5; xylose, 25990-60-7, 58-86-6; Alkalies; Cell Extracts; Polysaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v343_n4_p711_Prado

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Citas:

---------- APA ----------
Prado, H.J., Ciancia, M. & Matulewicz, M.C. (2008) . Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales). Carbohydrate Research, 343(4), 711-718.
http://dx.doi.org/10.1016/j.carres.2007.12.024
---------- CHICAGO ----------
Prado, H.J., Ciancia, M., Matulewicz, M.C. "Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)" . Carbohydrate Research 343, no. 4 (2008) : 711-718.
http://dx.doi.org/10.1016/j.carres.2007.12.024
---------- MLA ----------
Prado, H.J., Ciancia, M., Matulewicz, M.C. "Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)" . Carbohydrate Research, vol. 343, no. 4, 2008, pp. 711-718.
http://dx.doi.org/10.1016/j.carres.2007.12.024
---------- VANCOUVER ----------
Prado, H.J., Ciancia, M., Matulewicz, M.C. Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales). Carbohydr. Res. 2008;343(4):711-718.
http://dx.doi.org/10.1016/j.carres.2007.12.024