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Abstract:

Polysiphonia nigrescens was sequentially extracted with water at room temperature, 70 and 90 °C. The extracts were analyzed and the major one, isolated at 70 °C, was fractionated by ion-exchange chromatography, eluting with water and solutions of increasing sodium chloride concentration; five main fractions were separated. Structural analysis, carried out by methylation analysis and NMR spectroscopy, showed that four of these were partially cyclized agarans that were highly substituted on C-6 mainly with sulfate, although methyl ether and single stubs of β-d-xylose were found in minor proportions. A fifth fraction comprising 6-sulfated agarose was also isolated. The use of 2D NMR techniques allowed us to assign the 1H and 13C NMR resonances of the G6S→L6S diad for the first time. © 2008 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)
Autor:Prado, H.J.; Ciancia, M.; Matulewicz, M.C.
Filiación:Departamento de Química Orgánica, CIHIDECAR-CONICET, Facultad de Ciencias Exactas y Naturales, Pabellon 2, Ciudad Universitaria, 1428EGA Buenos Aires, Argentina
Cátedra de Química Orgánica, Departamento de Biología Aplicada y Alimentos (CIHIDECAR-CONICET), Facultad de Agronomía, Avenida San Martín 4453, 1417DSE Buenos Aires, Argentina
Palabras clave:Agarans; Algal polysaccharides; Polysiphonia nigrescens; Rhodomelaceae; Structural analysis; Sulfated galactans; Algal polysaccharides; Polysiphonia nigrescens; Rhodomelaceae; Sulfated galactans; Algae; Chromatographic analysis; Ion exchange; Methylation; Nuclear magnetic resonance spectroscopy; Sodium chloride; Polysaccharides; agaran; agarose; beta dextro xylose; dimethyl ether; hexamethonium bromide; polysaccharide; sodium chloride; sulfate; unclassified drug; water; xylose; article; carbon nuclear magnetic resonance; ceramiales; concentration (parameters); cyclization; drug isolation; elution; extraction; fractionation; ion exchange chromatography; methylation; nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; polysiphonia nigrescens; priority journal; proton nuclear magnetic resonance; rhodomelaceae; room temperature; seaweed; separation technique; structure analysis; Alkalies; Cell Extracts; Chromatography, Ion Exchange; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Polysaccharides; Seaweed; Temperature; algae; Ceramiales; Polysiphonia nigrescens; Rhodomelaceae
Año:2008
Volumen:343
Número:4
Página de inicio:711
Página de fin:718
DOI: http://dx.doi.org/10.1016/j.carres.2007.12.024
Título revista:Carbohydrate Research
Título revista abreviado:Carbohydr. Res.
ISSN:00086215
CODEN:CRBRA
CAS:agarose, 9012-36-6; dimethyl ether, 115-10-6; hexamethonium bromide, 55-97-0; sodium chloride, 7647-14-5; sulfate, 14808-79-8; water, 7732-18-5; xylose, 25990-60-7, 58-86-6; Alkalies; Cell Extracts; Polysaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v343_n4_p711_Prado

Referencias:

  • Batey, J.F., Turvey, J.R., (1975) Carbohydr. Res., 43, pp. 133-143
  • Usov, A.I., Ivanova, E.G., Shashkov, A.S., (1983) Bot. Mar., 27, pp. 285-294
  • Usov, A.I., Ivanova, E.G., (1987) Bot. Mar., 30, pp. 365-370
  • Miller, I.J., Furneaux, R.H., (1997) Bot. Mar., 40, pp. 333-339
  • Miller, I.J., (2003) Bot. Mar., 46, pp. 386-391
  • Matsuhiro, B., Rivas, P., (1993) J. Appl. Phycol., 5, pp. 45-51
  • Matsuhiro, B., (1996) Hydrobiologia, 326-327, pp. 481-489
  • Stortz, C.A., Cerezo, A.S., (2000) Curr. Top. Phytochem., 4, pp. 121-133
  • Yaphe, W., (1960) Anal. Chem., 32, pp. 1327-1330
  • Noseda, M.D., Tulio, S., Duarte, M.E.R., (1999) J. Appl. Phycol., 11, pp. 35-40
  • Knutsen, S.H., Myslabodski, D.E., Larsen, B., Usov, A.I., (1994) Bot. Mar., 37, pp. 163-169
  • Miller, I.J., Wong, H., Newman, R.H., (1982) Aust. J. Chem., 35, pp. 853-856
  • Welti, D., (1977) J. Chem. Res. (S), pp. 312-313
  • Usov, A.I., Bilan, M.I., Shashkov, A.S., (1997) Carbohydr. Res., 303, pp. 93-102
  • Lahaye, M., Yaphe, W., Rochas, C., (1985) Carbohydr. Res., 143, pp. 240-245
  • Zhang, Q., Li, N., Liu, X., Zhao, Z., Xu, Z., (2004) Carbohydr. Res., 339, pp. 105-111
  • Zhang, Q., Qi, H., Zhao, T., Deslandes, E., Ismaeli, N.M., Molloy, F., Critchley, A.T., (2005) Carbohydr. Res., 340, pp. 2447-2450
  • Knutsen, S.H., Grasdalen, H., (1987) Bot. Mar., 30, pp. 497-505
  • Errea, M.I., Matulewicz, M.C., (2003) Carbohydr. Res., 338, pp. 943-953
  • Falshaw, R., Furneaux, R.H., Miller, I.J., (1999) Bot. Mar., 42, pp. 431-435
  • Estevez, J.M., Ciancia, M., Cerezo, A.S., (2000) Carbohydr. Res., 325, pp. 287-299
  • Duarte, M.E.R., Noseda, M.D., Cardoso, M.A., Tulio, S., Cerezo, A.S., (2002) Carbohydr. Res., 337, pp. 1137-1144
  • Zibetti, R.G.M., Noseda, M.D., Cerezo, A.S., Duarte, M.E.R., (2005) Carbohydr. Res., 340, pp. 711-722
  • Murphy, V., Hughes, H., McLoughlin, P., (2007) Water Res., 41, pp. 731-740
  • Miller, I.J., (1997) Recent Res. Dev. Phytochem., 1, pp. 531-565
  • Sar, E., Pascual, M., Parma, A., (1984) Rev. Museo Plata - Sec. Bot., XIII, pp. 143-147
  • Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., (1956) Anal. Chem., 28, pp. 350-366
  • Navarro, D.R., Stortz, C.A., (2003) Carbohydr. Res., 338, pp. 2111-2118
  • Dodgson, K.S., Price, R.G., (1962) Biochem. J., 84, pp. 106-110
  • Lowry, O.H., Rosebrough, N.H., Farr, A.L., Randall, R.J., (1951) J. Biol. Chem., 193, pp. 265-275
  • Park, J.T., Johnson, M.J., (1949) J. Biol. Chem., 181, pp. 149-151
  • Stevenson, T.T., Furneaux, R.H., (1991) Carbohydr. Res., 210, pp. 277-298
  • Sweet, D.P., Shapira, R.H., Albersheim, P., (1975) Carbohydr. Res., 40, pp. 217-225
  • Cases, M.R., Cerezo, A.S., Stortz, C.A., (1995) Carbohydr. Res., 269, pp. 333-341
  • Ciucanu, I., Kerek, F., (1984) Carbohydr. Res., 131, pp. 209-217

Citas:

---------- APA ----------
Prado, H.J., Ciancia, M. & Matulewicz, M.C. (2008) . Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales). Carbohydrate Research, 343(4), 711-718.
http://dx.doi.org/10.1016/j.carres.2007.12.024
---------- CHICAGO ----------
Prado, H.J., Ciancia, M., Matulewicz, M.C. "Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)" . Carbohydrate Research 343, no. 4 (2008) : 711-718.
http://dx.doi.org/10.1016/j.carres.2007.12.024
---------- MLA ----------
Prado, H.J., Ciancia, M., Matulewicz, M.C. "Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)" . Carbohydrate Research, vol. 343, no. 4, 2008, pp. 711-718.
http://dx.doi.org/10.1016/j.carres.2007.12.024
---------- VANCOUVER ----------
Prado, H.J., Ciancia, M., Matulewicz, M.C. Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales). Carbohydr. Res. 2008;343(4):711-718.
http://dx.doi.org/10.1016/j.carres.2007.12.024