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Abstract:

The trans-sialidase from Trypanosoma cruzi (TcTS), the agent of Chagas' disease, is a unique enzyme involved in mammalian host-cell invasion. Since T. cruzi is unable to synthesize sialic acids de novo, TcTS catalyzes the transfer of α-(2→3)-sialyl residues from the glycoconjugates of the host to terminal β-galactopyranosyl units present on the surface of the parasite. TcTS also plays a key role in the immunomodulation of the infected host. Chronic Chagas' disease patients elicit TcTS-neutralizing antibodies that are able to inhibit the enzyme. N-Glycolylneuraminic acid has been detected in T. cruzi, and the trans-sialidase was pointed out as the enzyme involved in its incorporation from host glycoconjugates. However, N-glycolylneuraminic acid α-(2→3)-linked-containing oligosaccharides have not been analyzed as donors in the T. cruzi trans-sialidase reaction. In this paper we studied the ability of TcTS to transfer N-glycolylneuraminic acid from Neu5Gc(α2→3)Gal(β1→4)GlcβOCH2CH2N3 (1) and Neu5Gc(α2→3)Gal(β1→3)GlcNAcβOCH2CH2N3 (2) to lactitol, N-acetyllactosamine and lactose as acceptor substrates. Transfer from 1 was more efficient (50-65%) than from 2 (20-30%) for the three acceptors. The reactions were inhibited when the enzyme was preincubated with a neutralizing antibody. Km values were calculated for 1 and 2 and compared with 3′-sialyllactose using lactitol as acceptor substrate. Analysis was performed by high-performance anion-exchange (HPAEC) chromatography. A competitive transfer reaction of compound 1 in the presence of 3′-sialyllactose and N-acetyllactosamine showed a better transfer of Neu5Gc than of Neu5Ac. © 2007 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:The trans-sialidase from Trypanosoma cruzi efficiently transfers α-(2→3)-linked N-glycolylneuraminic acid to terminal β-galactosyl units
Autor:Agustí, R.; Giorgi, M.E.; de Lederkremer, R.M.
Filiación:CIHIDECAR, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon II, 1428 Buenos Aires, Argentina
Palabras clave:HPAEC; Neu5Gc transfer; trans-Sialidase; Trypanosoma cruzi; Antibodies; Biosynthesis; Carboxylic acids; Cells; Diseases; Mammals; Oligosaccharides; Chagas disease; Galactosyl units; Glycolylneuraminic acid; Trypanosoma cruzi; Enzyme inhibition; galactose; n glycoloylneuraminic acid; oligosaccharide; sialidase; anion exchange chromatography; article; Chagas disease; immunomodulation; priority journal; Trypanosoma cruzi; Animals; Galactose; Glycoproteins; Neuraminic Acids; Neuraminidase; Trypanosoma cruzi; Mammalia; Trypanosoma cruzi
Año:2007
Volumen:342
Número:16
Página de inicio:2465
Página de fin:2469
DOI: http://dx.doi.org/10.1016/j.carres.2007.07.018
Título revista:Carbohydrate Research
Título revista abreviado:Carbohydr. Res.
ISSN:00086215
CODEN:CRBRA
CAS:galactose, 26566-61-0, 50855-33-9, 59-23-4; n glycoloylneuraminic acid, 1113-83-3; sialidase, 9001-67-6; Galactose, 26566-61-0; Glycoproteins; N-glycolylneuraminic acid, 1113-83-3; Neuraminic Acids; Neuraminidase, EC 3.2.1.18; trans-sialidase, EC 3.2.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v342_n16_p2465_Agusti

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Citas:

---------- APA ----------
Agustí, R., Giorgi, M.E. & de Lederkremer, R.M. (2007) . The trans-sialidase from Trypanosoma cruzi efficiently transfers α-(2→3)-linked N-glycolylneuraminic acid to terminal β-galactosyl units. Carbohydrate Research, 342(16), 2465-2469.
http://dx.doi.org/10.1016/j.carres.2007.07.018
---------- CHICAGO ----------
Agustí, R., Giorgi, M.E., de Lederkremer, R.M. "The trans-sialidase from Trypanosoma cruzi efficiently transfers α-(2→3)-linked N-glycolylneuraminic acid to terminal β-galactosyl units" . Carbohydrate Research 342, no. 16 (2007) : 2465-2469.
http://dx.doi.org/10.1016/j.carres.2007.07.018
---------- MLA ----------
Agustí, R., Giorgi, M.E., de Lederkremer, R.M. "The trans-sialidase from Trypanosoma cruzi efficiently transfers α-(2→3)-linked N-glycolylneuraminic acid to terminal β-galactosyl units" . Carbohydrate Research, vol. 342, no. 16, 2007, pp. 2465-2469.
http://dx.doi.org/10.1016/j.carres.2007.07.018
---------- VANCOUVER ----------
Agustí, R., Giorgi, M.E., de Lederkremer, R.M. The trans-sialidase from Trypanosoma cruzi efficiently transfers α-(2→3)-linked N-glycolylneuraminic acid to terminal β-galactosyl units. Carbohydr. Res. 2007;342(16):2465-2469.
http://dx.doi.org/10.1016/j.carres.2007.07.018