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Abstract:

The polysaccharide extracted from cystocarpic Callophyllis variegata was fractionated with potassium chloride yielding three small fractions that precipitated in the ranges of 0-0.05 M KCl, 1.20-1.25 M KCl, and 1.80-2.00 M KCl, and a main product soluble in 2.00 M KCl. These fractions were analyzed, and as the first one contained very high amounts of protein, it was not studied further. Structural analysis of the rest of the fractions (F1-F3) was carried out by methylation, desulfation-methylation, IR, and 13C NMR spectroscopy. The results are consistent for F1 with a carrageenan-type backbone mainly constituted by β-D-galactose 2-sulfate linked to α-D-galactose 2,3,6-trisulfate and β-D-galactose 2,4-disulfate linked to 3,6-anhydro-D-galactose 2-sulfate as dominant diads. In F2 these diads are present together with low amounts of β-D-galactose 2-sulfate linked to 3,6-anhydro-D-galactose 2-sulfate, whose contribution becomes higher in F3. In addition, minor but significant amounts of L-galactose were detected. F1-F3 showed potent antiviral activity against herpes simplex types 1 and 2 and dengue type 2. © 2005 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
Autor:Rodríguez, M.C.; Merino, E.R.; Pujol, C.A.; Damonte, E.B.; Cerezo, A.S.; Matulewicz, M.C.
Filiación:Departamento de Biodiversidad Y Biología Experimental, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Departamento de Química Orgánica, CIHIDECAR-CONICET, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina
National Research Council of Argentina (CONICET), Argentina
Palabras clave:Antiviral activity; Callophyllis variegata; Carrageenans; Kallymeniaceae; Red algal polysaccharides; Sulfated galactans; Antiviral activity; Galactans; Nuclear magnetic resonance spectroscopy; Organic compounds; Potassium compounds; Carbohydrates; antivirus agent; carrageenan; galactan; galactose; polysaccharide; potassium chloride; protein; sulfate; antiviral activity; article; carbon nuclear magnetic resonance; chemical analysis; Dengue virus; drug isolation; fractionation; Herpes simplex virus 1; Herpes simplex virus 2; infrared spectroscopy; methylation; nuclear magnetic resonance spectroscopy; plant; precipitation; priority journal; seaweed; structure analysis; sulfation; Antiviral Agents; Carbon Isotopes; Galactans; Magnetic Resonance Spectroscopy; Plant Extracts; Seaweed; Sodium Hydroxide; Spectrophotometry, Infrared; algae; Callophyllis variegata; Dengue virus; Gigartinales; Herpes; Human herpesvirus 1; Human herpesvirus 2; Kallymeniaceae
Año:2005
Volumen:340
Número:18
Página de inicio:2742
Página de fin:2751
DOI: http://dx.doi.org/10.1016/j.carres.2005.10.001
Título revista:Carbohydrate Research
Título revista abreviado:Carbohydr. Res.
ISSN:00086215
CODEN:CRBRA
CAS:carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; galactan, 39300-87-3, 9037-55-2; galactose, 26566-61-0, 50855-33-9, 59-23-4; potassium chloride, 7447-40-7; protein, 67254-75-5; sulfate, 14808-79-8; Antiviral Agents; Carbon Isotopes; Galactans; Plant Extracts; Sodium Hydroxide, 1310-73-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v340_n18_p2742_Rodriguez

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Citas:

---------- APA ----------
Rodríguez, M.C., Merino, E.R., Pujol, C.A., Damonte, E.B., Cerezo, A.S. & Matulewicz, M.C. (2005) . Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales). Carbohydrate Research, 340(18), 2742-2751.
http://dx.doi.org/10.1016/j.carres.2005.10.001
---------- CHICAGO ----------
Rodríguez, M.C., Merino, E.R., Pujol, C.A., Damonte, E.B., Cerezo, A.S., Matulewicz, M.C. "Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)" . Carbohydrate Research 340, no. 18 (2005) : 2742-2751.
http://dx.doi.org/10.1016/j.carres.2005.10.001
---------- MLA ----------
Rodríguez, M.C., Merino, E.R., Pujol, C.A., Damonte, E.B., Cerezo, A.S., Matulewicz, M.C. "Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)" . Carbohydrate Research, vol. 340, no. 18, 2005, pp. 2742-2751.
http://dx.doi.org/10.1016/j.carres.2005.10.001
---------- VANCOUVER ----------
Rodríguez, M.C., Merino, E.R., Pujol, C.A., Damonte, E.B., Cerezo, A.S., Matulewicz, M.C. Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales). Carbohydr. Res. 2005;340(18):2742-2751.
http://dx.doi.org/10.1016/j.carres.2005.10.001