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Abstract:

In Xanthomonas campestris the genes involved in polysaccharide (xanthan) biosynthesis are located in a gene cluster (gum) of 16 kb. A Tn5 insertion mutant with a reduced slimy phenotype has been characterized. This mutant failed to produce the pentasaccharide repeating-unit of xanthan. Only three sugars were transferred to the prenyl phosphate intermediate. Several lines of evidence suggested that the lipid-associated saccharide was the trisaccharide reducing end of the pentasaccharide from the wild-type strain. This trisaccharide was built up from UDP-Glc and GDP-Man, and a glucose residue was at the reducing end, linked to an allylic prenol through a diphosphate bridge. Results from one- or two-stage reactions showed that the trisaccharide-P-P-polyprenol was the precursor of the polymer. This new polymer, a polytrisaccharide, was detected also in vivo. The transposon responsible for the mutation was located within gumK gene. Therefore, this gene encodes for the glycosyltransferase IV, which catalyses the transfer of glucuronic acid to the lipid-linked β-D-Manp-(1 → 3)-β-D-Glcp-(1 → 4)-β-D-Glcp trisaccharide. A recombinant plasmid with the whole gum cluster restored the wild type phenotype. © 2002 Elsevier Science Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris
Autor:Vojnov, A.A.; Bassi, D.E.; Daniels, M.J.; Dankert, M.A.
Filiación:Instituto De Investigaciones Bioquímicas Fundación Campomar, Facultad De Ciencias Exactas Y Naturales, Universidad De Buenos Aires and Consejo Nacional De Investigaciones Cientificas Y Tecnicas, Av. Patricias Argentinas 435, 1405, Buenos Aires, Argentina
Sainsbury Laboratory at the John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, United Kingdom
Palabras clave:Biosynthesis; Gum genes; Polysaccharide; Xanthan; Biosynthesis; Cellulose; Genes; Glucose; Lipids; Phosphates; Polymers; Polysaccharides; Sugars; Mutant strains; Wild type phenotypes; Carbohydrates; alkaline phosphatase; cellulose; edetic acid; glucose; glucuronic acid; glycosyltransferase; glycosyltransferase iv; pentasaccharide; polymer; pyrophosphate; trisaccharide; unclassified drug; xanthan; article; biosynthesis; carbohydrate analysis; catalysis; controlled study; gene cluster; gene mutation; nonhuman; phenotype; plasmid; priority journal; radioactivity; structure analysis; transposon; Xanthomonas campestris; Bacterial Proteins; Cellulose; DNA Transposable Elements; Glucuronic Acid; Glycosyltransferases; Lipopolysaccharides; Oligosaccharides; Polysaccharides, Bacterial; Xanthomonas campestris; insertion sequences; transposons; Xanthomonas; Xanthomonas campestris
Año:2002
Volumen:337
Número:4
Página de inicio:315
Página de fin:326
DOI: http://dx.doi.org/10.1016/S0008-6215(01)00322-6
Título revista:Carbohydrate Research
Título revista abreviado:Carbohydr. Res.
ISSN:00086215
CODEN:CRBRA
CAS:Bacterial Proteins; Cellulose, 9004-34-6; DNA Transposable Elements; Glucuronic Acid, 576-37-4; Glycosyltransferases, EC 2.4.-; GumD protein, Xanthomonas campestris; lipid-linked oligosaccharides; Lipopolysaccharides; Oligosaccharides; Polysaccharides, Bacterial; xanthan gum, 11138-66-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v337_n4_p315_Vojnov

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Citas:

---------- APA ----------
Vojnov, A.A., Bassi, D.E., Daniels, M.J. & Dankert, M.A. (2002) . Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris. Carbohydrate Research, 337(4), 315-326.
http://dx.doi.org/10.1016/S0008-6215(01)00322-6
---------- CHICAGO ----------
Vojnov, A.A., Bassi, D.E., Daniels, M.J., Dankert, M.A. "Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris" . Carbohydrate Research 337, no. 4 (2002) : 315-326.
http://dx.doi.org/10.1016/S0008-6215(01)00322-6
---------- MLA ----------
Vojnov, A.A., Bassi, D.E., Daniels, M.J., Dankert, M.A. "Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris" . Carbohydrate Research, vol. 337, no. 4, 2002, pp. 315-326.
http://dx.doi.org/10.1016/S0008-6215(01)00322-6
---------- VANCOUVER ----------
Vojnov, A.A., Bassi, D.E., Daniels, M.J., Dankert, M.A. Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris. Carbohydr. Res. 2002;337(4):315-326.
http://dx.doi.org/10.1016/S0008-6215(01)00322-6