Abstract:
Several commercial sulfated neocarrabiose oligosaccharides were analyzed by matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry (UV-MALDI-TOF-MS). UV-MALDI-TOF-MS was carried out in the linear and reflectron modes and, as routine, in both the positive- and negative-ion modes. 2,5-Dihydroxybenzoic acid and nor-harmane were used as matrices. In the positive- and negative-ion modes, with both matrices, peaks corresponding to (M+Na)+ and (M-Na)- ions, respectively, were obtained, with only some signals due to glycosidic linkage cleavages (prompt fragmentation). With 2,5-dihydroxybenzoic acid abundant matrix signals were observed; nor-harmane afforded very few matrix signals in both ion modes, but more desulfation (prompt fragmentation) of the compounds occurred. When the desorption/ionization process was highly efficient, the post-source decay (PSD) fragmentation patterns were also investigated; most of the fragments detected derived from glycosidic linkage cleavages. Electrospray-ionization time-of-flight mass spectrometry (ESI-TOF-MS) in the negative-ion mode confirmed, with the observation of the (M-Na)- and the multiply charged anions, the identity and the purity of the samples. © 2002 Elsevier Science Ltd. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides |
Autor: | Fukuyama, Y.; Ciancia, M.; Nonami, H.; Cerezo, A.S.; Erra-Balsells, R.; Matulewicz, M.C. |
Filiación: | Plant Biophysics/Biochemistry Research Laboratory, College of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan Departamento De Química Orgánica, CIHIDECAR-CONICET, Pabellón 2-Ciudad Universitaria, 1428 Buenos Aires, Argentina Departamento De Química Orgánica, Facultad De Ciencias Exactas Y Naturales, Pabellón 2-Ciudad Universitaria, 1428 Buenos Aires, Argentina
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Palabras clave: | ESI-TOF-MS; kappa-Carrageenan oligosaccharides; PSD UV-MALDI-TOF-MS; Sulfated neocarrabiose oligosaccharides; Sulfated oligosaccharides; UV-MALDI-TOF-MS; Carboxylic acids; Ionization; Mass spectrometry; Ultraviolet radiation; Fragmentation; Oligomers; gentisic acid; harman; neocarrabiose 4 sulfate; neocarradodecaose 4,4,4,4,4,4 hexasulfate; neocarrahexaose 2,4,4,4 tetrasulfate; neocarrahexaose 4,4,4 trisulfate; neocarraoctaose 4,4,4,4 tetrasulfate; neocarratetraose 4 sulfate; neocarratetraose 4,4 disulfate; oligosaccharide; unclassified drug; analytic method; article; carbohydrate analysis; chemical bond; desorption; ionization; matrix assisted laser desorption ionization time of flight mass spectrometry; priority journal; sulfation; Carbohydrate Sequence; Carrageenan; Molecular Sequence Data; Oligosaccharides; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfates; Ultraviolet Rays |
Año: | 2002
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Volumen: | 337
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Número: | 17
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Página de inicio: | 1553
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Página de fin: | 1562
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DOI: |
http://dx.doi.org/10.1016/S0008-6215(02)00230-6 |
Título revista: | Carbohydrate Research
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Título revista abreviado: | Carbohydr. Res.
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ISSN: | 00086215
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CODEN: | CRBRA
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CAS: | Carrageenan, 9000-07-1; neocarrabiose, 6215-96-9; Oligosaccharides; Sulfates
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v337_n17_p1553_Fukuyama |
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Citas:
---------- APA ----------
Fukuyama, Y., Ciancia, M., Nonami, H., Cerezo, A.S., Erra-Balsells, R. & Matulewicz, M.C.
(2002)
. Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides. Carbohydrate Research, 337(17), 1553-1562.
http://dx.doi.org/10.1016/S0008-6215(02)00230-6---------- CHICAGO ----------
Fukuyama, Y., Ciancia, M., Nonami, H., Cerezo, A.S., Erra-Balsells, R., Matulewicz, M.C.
"Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides"
. Carbohydrate Research 337, no. 17
(2002) : 1553-1562.
http://dx.doi.org/10.1016/S0008-6215(02)00230-6---------- MLA ----------
Fukuyama, Y., Ciancia, M., Nonami, H., Cerezo, A.S., Erra-Balsells, R., Matulewicz, M.C.
"Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides"
. Carbohydrate Research, vol. 337, no. 17, 2002, pp. 1553-1562.
http://dx.doi.org/10.1016/S0008-6215(02)00230-6---------- VANCOUVER ----------
Fukuyama, Y., Ciancia, M., Nonami, H., Cerezo, A.S., Erra-Balsells, R., Matulewicz, M.C. Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides. Carbohydr. Res. 2002;337(17):1553-1562.
http://dx.doi.org/10.1016/S0008-6215(02)00230-6