Artículo
Abstract:
Incubation of liver microsomes with dolichyl-d-glucosyl-14C monophosphate led to the labelling of an endogenous acceptor. This compound seems to be also a dolichol derivative. It contains a high-molecular weight oligosaccharide bound to dolichol through a phosphate or pyrophosphate bond. Various treatments of the labelled oligosaccharide afforded further information on its structure: Reduction with sodium borohydride, followed by acid hydrolysis gave only radioactive d-glucose indicating that the labelled d-glucose is not incorporated at the reducing end of the oligosaccharide. The percentage of radioactivity, liberated as formic acid after periodate oxidation, indicates that more than one molecule of d-glucose is incorporated and that at least one of them is inside the oligosaccharide chain. Alkaline treatment of the otherwise neutral oligosaccharide gave two positively charged derivatives which could be neutralized by N-acetylation, indicating the presence of two hexosamine residues. The oligosaccharides isolated from different tissues by the same method as that used for rat liver, were similar as judged by their migration in paper chromatography and by the pattern of products liberated by acetolysis. © 1973.
Registro:
| Documento: | Artículo |
| Título: | Further studies on a glycolipid formed from dolichyl-d-glucosyl monophosphate |
| Autor: | Parodi, A.J.; Staneloni, R.; Cantarella, A.I.; Leloir, L.F.; Behrens, N.H.; Carminatti, H.; Levy, J.A. |
| Filiación: | Instituto de Investigaciones Bioquímicas, Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires, Argentina |
| Palabras clave: | borane derivative; carbon; concanavalin A; fatty alcohol; glucose phosphate; glycolipid; oligosaccharide; periodic acid; terpene; tritium; animal; article; binding site; liver; liver microsome; metabolism; oxidation reduction reaction; paper chromatography; rat; swine; time; Animal; Binding Sites; Borohydrides; Carbon Isotopes; Chromatography, Paper; Concanavalin A; Fatty Alcohols; Glucosephosphates; Glycolipids; Liver; Microsomes, Liver; Oligosaccharides; Oxidation-Reduction; Periodic Acid; Rats; Swine; Terpenes; Time Factors; Tritium |
| Año: | 1973 |
| Volumen: | 26 |
| Número: | 2 |
| Página de inicio: | 393 |
| Página de fin: | 400 |
| DOI: | http://dx.doi.org/10.1016/S0008-6215(00)84527-9 |
| Título revista: | Carbohydrate Research |
| Título revista abreviado: | Carbohydr. Res. |
| ISSN: | 00086215 |
| CODEN: | CRBRA |
| CAS: | carbon, 7440-44-0; concanavalin A, 11028-71-0; periodic acid, 10450-60-9, 13444-71-8; tritium, 10028-17-8; Borohydrides; Carbon Isotopes; Concanavalin A, 11028-71-0; Fatty Alcohols; Glucosephosphates; Glycolipids; Oligosaccharides; Periodic Acid, 10450-60-9; Terpenes; Tritium, 10028-17-8 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v26_n2_p393_Parodi |
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Citas:
---------- APA ----------
Parodi, A.J., Staneloni, R., Cantarella, A.I., Leloir, L.F., Behrens, N.H., Carminatti, H. & Levy, J.A. (1973) . Further studies on a glycolipid formed from dolichyl-d-glucosyl monophosphate. Carbohydrate Research, 26(2), 393-400.http://dx.doi.org/10.1016/S0008-6215(00)84527-9
---------- CHICAGO ----------
Parodi, A.J., Staneloni, R., Cantarella, A.I., Leloir, L.F., Behrens, N.H., Carminatti, H., et al. "Further studies on a glycolipid formed from dolichyl-d-glucosyl monophosphate" . Carbohydrate Research 26, no. 2 (1973) : 393-400.http://dx.doi.org/10.1016/S0008-6215(00)84527-9
---------- MLA ----------
Parodi, A.J., Staneloni, R., Cantarella, A.I., Leloir, L.F., Behrens, N.H., Carminatti, H., et al. "Further studies on a glycolipid formed from dolichyl-d-glucosyl monophosphate" . Carbohydrate Research, vol. 26, no. 2, 1973, pp. 393-400.http://dx.doi.org/10.1016/S0008-6215(00)84527-9
---------- VANCOUVER ----------
Parodi, A.J., Staneloni, R., Cantarella, A.I., Leloir, L.F., Behrens, N.H., Carminatti, H., et al. Further studies on a glycolipid formed from dolichyl-d-glucosyl monophosphate. Carbohydr. Res. 1973;26(2):393-400.http://dx.doi.org/10.1016/S0008-6215(00)84527-9
