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Abstract:

A study of nucleotides and nucleotide sugars in mature oocytes of Bufo arenarum has been performed. Two samples of oocytes from ovulations of ten females each were analyzed by ion-exchange chromatography and the following nucleotides and nucleotide sugars found2 2 The following abbreviations are used: ADP, adenosine 5′-pyrophosphate; ATP, adenosine 5′-triphosphate; CTP, cytidine 5′-triphosphate; GDP, guanosine 5′-pyrophosphate; GTP, guanosine 5′-triphosphate; NAD, nicotinamide adenine dinucleotide; NADP, nicotinamide adenine dinucleotide phosphate; UDP, uridine 5′-pyrophosphate; UDPG, uridine 5′-(d-glucosyl pyrophosphate; UDPGA, uridine 5′-(d-glucosyluronic acid pyrophosphate); UDPGalNAc, uridine 5′-(2-acetamido-2-deoxy-d-galactosyl pyrophosphate), UDPGNAc, uridine 5′-(2-acetamido-2-deoxy-d-glucosyl pyrophosphate), and UTP, uridine 5′-triphosphate.: NAD, UDPGNAc, UDPGalNAc, UDPG, UDP, NADP, CTP, UTP, UDPGA, ADP, GDP, GTP, and ATP. The nucleotides and sugar-nucleotides were identified on the basis of chemical, enzymic, and chromatographic analysis. To minimize the decomposition of unstable substances, the extraction was performed with trichloroacetic acid in the cold, the contact of the sample with the acid was minimal and ammonium chloride was used for the elution from the Dowex 1-resin. In order to detect compounds present in minute amounts, very large samples were used (60,000 to 120,000 oocytes). A nucleotide of complex structure, which binds weakly to the column, was detected in the two samples analyzed. © 1973.

Registro:

Documento: Artículo
Título:Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes
Autor:Fernández de Recondo, M.E.; Maggesse, M.C.; Guerrero, G.; C. Spaizman, R.; Recondo, E.F.
Filiación:Embriologia Animal y Biologia Molecular, Departamentos de Ciencias Biológicas y Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, Buenos Aires Argentina
Palabras clave:carbon; nucleoside diphosphate sugar; nucleotide; animal; article; embryology; female; fertilization; ion exchange chromatography; oocyte; ovary; paper chromatography; paper electrophoresis; toad; Animal; Bufo arenarum; Carbon Isotopes; Chromatography, Ion Exchange; Chromatography, Paper; Electrophoresis, Paper; Female; Fertilization; Nucleoside Diphosphate Sugars; Nucleotides; Ovary; Ovum
Año:1973
Volumen:26
Número:2
Página de inicio:365
Página de fin:375
DOI: http://dx.doi.org/10.1016/S0008-6215(00)84524-3
Título revista:Carbohydrate Research
Título revista abreviado:Carbohydr. Res.
ISSN:00086215
CODEN:CRBRA
CAS:carbon, 7440-44-0; Carbon Isotopes; Nucleoside Diphosphate Sugars; Nucleotides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v26_n2_p365_FernandezdeRecondo

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Citas:

---------- APA ----------
Fernández de Recondo, M.E., Maggesse, M.C., Guerrero, G., C. Spaizman, R. & Recondo, E.F. (1973) . Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes. Carbohydrate Research, 26(2), 365-375.
http://dx.doi.org/10.1016/S0008-6215(00)84524-3
---------- CHICAGO ----------
Fernández de Recondo, M.E., Maggesse, M.C., Guerrero, G., C. Spaizman, R., Recondo, E.F. "Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes" . Carbohydrate Research 26, no. 2 (1973) : 365-375.
http://dx.doi.org/10.1016/S0008-6215(00)84524-3
---------- MLA ----------
Fernández de Recondo, M.E., Maggesse, M.C., Guerrero, G., C. Spaizman, R., Recondo, E.F. "Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes" . Carbohydrate Research, vol. 26, no. 2, 1973, pp. 365-375.
http://dx.doi.org/10.1016/S0008-6215(00)84524-3
---------- VANCOUVER ----------
Fernández de Recondo, M.E., Maggesse, M.C., Guerrero, G., C. Spaizman, R., Recondo, E.F. Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes. Carbohydr. Res. 1973;26(2):365-375.
http://dx.doi.org/10.1016/S0008-6215(00)84524-3