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A macromolecular (1→4)-α-d-[14C]glucan-protein complex was synthesized with a rat liver preparation and uridine diphosphate d-[14C]glucose. The size of the complex is contributed by both the protein and the (1→4)-α-d-glucosyl-oligomer components. Iodoacetamide treatment did not change the migration properties on Bio-Gel A-50m. Therefore, disulfide bonds linking glucan-protein subunits seem not to be involved. The [14C]glucan-protein, precipitated by diluted trichloroacetic acid, was digested by α-amylase, phosphorylase a, and proteases. The extent of proteolysis was greater for a complex having fewer d-glucose units incorporated. After proteolytic digestion of that complex, the labeled fragments behaved on electrophoresis, and ion-exchange and gel chromatography as [14C]glucosylated peptides. These findings support previous conclusions that the primer for liver glycogen synthesis is a protein on which glycogen is built up by covalent attachment. © 1986.


Documento: Artículo
Título:A (1→4)-α-d-glucan-protein involved in liver glycogen biosynthesis
Autor:Krisman, C.R.; Geremia, R.A.; Whelan, W.J.
Filiación:Instituto de Investigaciones Bioquímicas Fundacion Compamar, Facultad de Ciencias Exactas y Naturales, CONICET, Antonio Machado 151, 1405 Buenos Aires, Argentina
Department of Biochemistry, University of Miami School of Medicine, Miami, FL 33101, United States
Palabras clave:carbon; glucan; glycoprotein; uridine diphosphate glucose; animal; article; biosynthesis; enzymology; gel chromatography; glycogen liver level; ion exchange chromatography; liver; metabolism; paper electrophoresis; rat; Animal; Carbon Radioisotopes; Chromatography, Gel; Chromatography, Ion Exchange; Electrophoresis, Paper; Glucans; Glycoproteins; Liver; Liver Glycogen; Rats; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Uridine Diphosphate Glucose
Página de inicio:35
Página de fin:45
Título revista:Carbohydrate Research
Título revista abreviado:Carbohydr. Res.
CAS:carbon, 7440-44-0; glucan, 9012-72-0, 9037-91-6; uridine diphosphate glucose, 133-89-1; Carbon Radioisotopes; Glucans; Glycoproteins; Liver Glycogen; Uridine Diphosphate Glucose, 133-89-1


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---------- APA ----------
Krisman, C.R., Geremia, R.A. & Whelan, W.J. (1986) . A (1→4)-α-d-glucan-protein involved in liver glycogen biosynthesis. Carbohydrate Research, 149(1), 35-45.
---------- CHICAGO ----------
Krisman, C.R., Geremia, R.A., Whelan, W.J. "A (1→4)-α-d-glucan-protein involved in liver glycogen biosynthesis" . Carbohydrate Research 149, no. 1 (1986) : 35-45.
---------- MLA ----------
Krisman, C.R., Geremia, R.A., Whelan, W.J. "A (1→4)-α-d-glucan-protein involved in liver glycogen biosynthesis" . Carbohydrate Research, vol. 149, no. 1, 1986, pp. 35-45.
---------- VANCOUVER ----------
Krisman, C.R., Geremia, R.A., Whelan, W.J. A (1→4)-α-d-glucan-protein involved in liver glycogen biosynthesis. Carbohydr. Res. 1986;149(1):35-45.