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Abstract:

BACKGROUND. Although alterations of the bone marrow (BM) fibroblast colony-forming cells are involved in the development of diverse hematologic disorders, these progenitors still have not been well characterized in patients with solid tumors. METHODS. The incidence of fibroblast colony- forming units (CFU-F) was evaluated in the cultures of unseparated and fractionated light density BM mononuclear cells (MC) from 25 consecutive untreated lung carcinoma patients (LCP) and 16 normal controls (NC). Unseparated MC also were cultured in the presence of indomethacin (10-6 M). Finally, the authors evaluated the spontaneous production of prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) in culture conditioned mediums of unseparated MC by radioimmunoassay and enzyme-linked immunoadsorbent assay methodology, respectively. RESULTS. A decreased number of CFU-F was observed in unseparated and fractionated (adherent and nonadherent) light density MC cultures from LCP compared with NC. When unseparated MC of LCP were treated with indomethacin, a slightly increase in the number of CFU-F was found. Adherent MC (stromal cells) achieved confluence only in 44% of LCP primary cultures compared with 100% of NC. Overproduction of PGE2 and TNF-α was found in the conditioned mediums of LCP compared with the mean values obtained in NC (P < 0.05 and P < 0.02, respectively). CONCLUSIONS. The lack of confluence and suppression of CFU-F in BM of LCP may be related to the increase production of PGE2 and TNF-α. Future investigation will allow the determination of how these modifications influence tumor cell growth and will prove if more alterations of the hematopoietic microenvironment imply a worse prognosis.

Registro:

Documento: Artículo
Título:Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma
Autor:Chasseing, N.A.; Bordenave, R.H.; Bullorsky, E.O.; Diaz, N.B.; Stemmelin, G.R.; Rumi, L.S.
Filiación:Consejo Nac. Invest. Cie. Tec. R., Buenos Aires, Argentina
Inst. de Biol. y Med. Experimental, Buenos Aires, Argentina
Department of Oncology, Hospital Zonal de Agudos I. Iriarte, Buenos Aires, Argentina
Dept. Hematol. Bone Marrow T., Hospital Británico, Buenos Aires, Argentina
Hematology Division, Department of Clinical Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Inst. de Biol. y Med. Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Bone marrow fibroblast; Lung carcinoma; Prostaglandin E2; Tumor necrosis factor-α; prostaglandin e2; tumor necrosis factor; advanced cancer; article; bone marrow culture; colony forming unit f; controlled study; enzyme linked immunosorbent assay; human; human tissue; lung non small cell cancer; mononuclear cell; priority journal; radioimmunoassay; Bone Marrow Cells; Carcinoma, Non-Small-Cell Lung; Case-Control Studies; Colony-Forming Units Assay; Culture Media, Conditioned; Dinoprostone; Female; Fibroblasts; Humans; Lung Neoplasms; Male; Stem Cells; Tumor Necrosis Factor-alpha
Año:1997
Volumen:80
Número:10
Página de inicio:1914
Página de fin:1919
DOI: http://dx.doi.org/10.1002/(SICI)1097-0142(19971115)80:10<1914::AID-CNCR7>3.0.CO;2-N
Título revista:Cancer
Título revista abreviado:CANCER
ISSN:0008543X
CODEN:CANCA
CAS:Culture Media, Conditioned; Dinoprostone, 363-24-6; Tumor Necrosis Factor-alpha
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0008543X_v80_n10_p1914_Chasseing

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Citas:

---------- APA ----------
Chasseing, N.A., Bordenave, R.H., Bullorsky, E.O., Diaz, N.B., Stemmelin, G.R. & Rumi, L.S. (1997) . Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma. Cancer, 80(10), 1914-1919.
http://dx.doi.org/10.1002/(SICI)1097-0142(19971115)80:10<1914::AID-CNCR7>3.0.CO;2-N
---------- CHICAGO ----------
Chasseing, N.A., Bordenave, R.H., Bullorsky, E.O., Diaz, N.B., Stemmelin, G.R., Rumi, L.S. "Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma" . Cancer 80, no. 10 (1997) : 1914-1919.
http://dx.doi.org/10.1002/(SICI)1097-0142(19971115)80:10<1914::AID-CNCR7>3.0.CO;2-N
---------- MLA ----------
Chasseing, N.A., Bordenave, R.H., Bullorsky, E.O., Diaz, N.B., Stemmelin, G.R., Rumi, L.S. "Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma" . Cancer, vol. 80, no. 10, 1997, pp. 1914-1919.
http://dx.doi.org/10.1002/(SICI)1097-0142(19971115)80:10<1914::AID-CNCR7>3.0.CO;2-N
---------- VANCOUVER ----------
Chasseing, N.A., Bordenave, R.H., Bullorsky, E.O., Diaz, N.B., Stemmelin, G.R., Rumi, L.S. Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma. CANCER. 1997;80(10):1914-1919.
http://dx.doi.org/10.1002/(SICI)1097-0142(19971115)80:10<1914::AID-CNCR7>3.0.CO;2-N