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Abstract:

Stress activates the synthesis and secretion of catecholamines and adrenal glucocorticoids, increasing their circulating levels. In vivo, hepatic 11β-hydroxysteroid dehydrogenase 1 (HSD1) stimulates the shift of 11-dehydrocorticosterone to corticosterone, enhancing active glucocorticoids at tissue level. We studied the effect of 3 types of stress, 1 induced by bucogastric overload with 200 mmol/L HCl causing metabolic acidosis (HCl), the second induced by bucogastric overload with 0.45% NaCl (NaCl), and the third induced by simulated overload (cannula), on the kinetics of hepatic HSD1 of rats and their influence on the activity of the gluconeogenic enzyme phosphoenolpyruvate carboxykinase, glycemia, and glycogen deposition. Compared with unstressed controls, all types of stress significantly increased HSD1 activity (146% cannula, 130% NaCl, and 253% HCl), phosphoenolpyruvate carboxykinase activity (51% cannula, 48% NaCl, and 86% HCl), and glycemia (29% cannula, 30% NaCl, and 41% HCl), but decreased hepatic glycogen (68% cannula, 68% NaCl, and 78% HCl). Owing to these results, we suggest the following events occur when stress is induced: an increase in hepatic HSD1 activity, augmented active glucocorticoid levels, increased gluconeogenesis, and glycemia. Also involved are the multiple events indirectly related to glucocorticoids, which lead to the depletion of hepatic glycogen deposits, thereby contributing to increased glycemia. This new approach shows that stress increments the activity of hepatic HSD1 and suggests that this enzyme could be involved in the development of the Metabolic Syndrome. © 2006 NRC.

Registro:

Documento: Artículo
Título:Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism
Autor:Altuna, M.E.; Lelli, S.M.; San Martín De Viale, L.C.; Damasco, M.C.
Filiación:Laboratorio de Esteroides, Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Universidad de Buenos Aires, C1428EGA, Buenos Aires, Argentina
Laboratorio de Disturbios Metabolicos Por Xenobioticos, Salud Humana Y Medio Ambiente (DIMXSA), Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Universidad de Buenos Aires, C1428EGA, Buenos Aires, Argentina
Palabras clave:Carbohydrate metabolism; Glucocorticoids; Hepatic HSD1; PEPCK; Rat; Stress; 11beta hydroxysteroid dehydrogenase 1; corticosterone; dehydrocorticosterone; glucocorticoid; glycogen; hydrochloric acid; oxidoreductase; phosphoenolpyruvate carboxykinase (GTP); sodium chloride; unclassified drug; animal experiment; article; cannula; carbohydrate metabolism; controlled study; enzyme activity; enzyme kinetics; gluconeogenesis; glucose blood level; male; metabolic acidosis; nonhuman; priority journal; rat; stress; 11-beta-Hydroxysteroid Dehydrogenase Type 1; 11-beta-Hydroxysteroid Dehydrogenases; Animals; Blood Glucose; Carbohydrate Metabolism; Corticosterone; Cytosol; Gluconeogenesis; Kinetics; Liver; Liver Glycogen; Male; Microsomes, Liver; Phosphoenolpyruvate Carboxykinase (ATP); Rats; Rats, Sprague-Dawley; Stress, Psychological
Año:2006
Volumen:84
Número:10
Página de inicio:977
Página de fin:984
DOI: http://dx.doi.org/10.1139/Y06-046
Título revista:Canadian Journal of Physiology and Pharmacology
Título revista abreviado:Can. J. Physiol. Pharmacol.
ISSN:00084212
CODEN:CJPPA
CAS:corticosterone, 50-22-6; dehydrocorticosterone, 72-23-1; glycogen, 9005-79-2; hydrochloric acid, 7647-01-0; oxidoreductase, 9035-73-8, 9035-82-9, 9037-80-3, 9055-15-6; phosphoenolpyruvate carboxykinase (GTP), 9013-08-5; sodium chloride, 7647-14-5; 11-beta-Hydroxysteroid Dehydrogenase Type 1, 1.1.1.146; 11-beta-Hydroxysteroid Dehydrogenases, 1.1.1.146; Blood Glucose; Corticosterone, 50-22-6; Liver Glycogen; Phosphoenolpyruvate Carboxykinase (ATP), 4.1.1.49
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v84_n10_p977_Altuna

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Citas:

---------- APA ----------
Altuna, M.E., Lelli, S.M., San Martín De Viale, L.C. & Damasco, M.C. (2006) . Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism. Canadian Journal of Physiology and Pharmacology, 84(10), 977-984.
http://dx.doi.org/10.1139/Y06-046
---------- CHICAGO ----------
Altuna, M.E., Lelli, S.M., San Martín De Viale, L.C., Damasco, M.C. "Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism" . Canadian Journal of Physiology and Pharmacology 84, no. 10 (2006) : 977-984.
http://dx.doi.org/10.1139/Y06-046
---------- MLA ----------
Altuna, M.E., Lelli, S.M., San Martín De Viale, L.C., Damasco, M.C. "Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism" . Canadian Journal of Physiology and Pharmacology, vol. 84, no. 10, 2006, pp. 977-984.
http://dx.doi.org/10.1139/Y06-046
---------- VANCOUVER ----------
Altuna, M.E., Lelli, S.M., San Martín De Viale, L.C., Damasco, M.C. Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism. Can. J. Physiol. Pharmacol. 2006;84(10):977-984.
http://dx.doi.org/10.1139/Y06-046