Abstract:
This work studied the effect of stresses produced by simulated gavage or gavage with 200 mmol/L HCl two hours before adrenal extraction, on the activities of the 11β-hydroxysteroid dehydrogenase 1 and 11β-hydroxysteroid dehydrogenase 2 isoforms present in the rat adrenal gland. These activities were determined on immediately prepared adrenal microsomes following incubations with 3H-corticosterone and NAD + or NADP+. 11-dehydrocorticosterone was measured as an end-product by TLC, and controls were adrenal microsomes from rats kept under basal (unstressed) conditions. 11β-hydroxysteroid dehydrogenase 1 activity, but not 11β-hydroxysteroid dehydrogenase 2 activity, was increased under both stress-conditions. Homeostatically, the stimulation of 11β- hydroxysteroid dehydrogenase 1 activity would increase the supply of glucocorticoids. These, in turn, would activate the enzyme phenylethanolamine N-methyl transferase, thereby improving the synthesis of epinephrine as part of the stress-response.
Registro:
Documento: |
Artículo
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Título: | Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress |
Autor: | Zallocchi, M.; Matković, L.; Damasco, M.C. |
Filiación: | Dpto. Quím. Biológica, Pabellón 2, Ciudad Universitaria, Cd. Auton. de (1428) Buenos Aires, Argentina
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Palabras clave: | Acidosis; Adrenal; HSD; Stress; 11beta hydroxysteroid dehydrogenase; adrenalin; corticosterone; dehydrocorticosterone; glucocorticoid; hydrochloric acid; isoenzyme; nicotinamide adenine dinucleotide; nicotinamide adenine dinucleotide phosphate; phenylethanolamine n methyltransferase; adrenal gland; animal experiment; animal tissue; article; controlled study; enzyme activation; enzyme activity; experimental rat; feeding; homeostasis; male; measurement; microsome; nonhuman; priority journal; protein synthesis; rat; simulation; stress; thin layer chromatography |
Año: | 2004
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Volumen: | 82
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Número: | 6
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Página de inicio: | 422
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Página de fin: | 425
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DOI: |
http://dx.doi.org/10.1139/y04-035 |
Título revista: | Canadian Journal of Physiology and Pharmacology
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Título revista abreviado: | Can. J. Physiol. Pharmacol.
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ISSN: | 00084212
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CODEN: | CJPPA
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CAS: | 11beta hydroxysteroid dehydrogenase, 9041-46-7; corticosterone, 50-22-6; dehydrocorticosterone, 72-23-1; hydrochloric acid, 7647-01-0; nicotinamide adenine dinucleotide, 53-84-9; nicotinamide adenine dinucleotide phosphate, 53-59-8; phenylethanolamine n methyltransferase, 9037-68-7
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v82_n6_p422_Zallocchi |
Referencias:
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Citas:
---------- APA ----------
Zallocchi, M., Matković, L. & Damasco, M.C.
(2004)
. Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress. Canadian Journal of Physiology and Pharmacology, 82(6), 422-425.
http://dx.doi.org/10.1139/y04-035---------- CHICAGO ----------
Zallocchi, M., Matković, L., Damasco, M.C.
"Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress"
. Canadian Journal of Physiology and Pharmacology 82, no. 6
(2004) : 422-425.
http://dx.doi.org/10.1139/y04-035---------- MLA ----------
Zallocchi, M., Matković, L., Damasco, M.C.
"Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress"
. Canadian Journal of Physiology and Pharmacology, vol. 82, no. 6, 2004, pp. 422-425.
http://dx.doi.org/10.1139/y04-035---------- VANCOUVER ----------
Zallocchi, M., Matković, L., Damasco, M.C. Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress. Can. J. Physiol. Pharmacol. 2004;82(6):422-425.
http://dx.doi.org/10.1139/y04-035