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Abstract:

This work studied the effect of stresses produced by simulated gavage or gavage with 200 mmol/L HCl two hours before adrenal extraction, on the activities of the 11β-hydroxysteroid dehydrogenase 1 and 11β-hydroxysteroid dehydrogenase 2 isoforms present in the rat adrenal gland. These activities were determined on immediately prepared adrenal microsomes following incubations with 3H-corticosterone and NAD + or NADP+. 11-dehydrocorticosterone was measured as an end-product by TLC, and controls were adrenal microsomes from rats kept under basal (unstressed) conditions. 11β-hydroxysteroid dehydrogenase 1 activity, but not 11β-hydroxysteroid dehydrogenase 2 activity, was increased under both stress-conditions. Homeostatically, the stimulation of 11β- hydroxysteroid dehydrogenase 1 activity would increase the supply of glucocorticoids. These, in turn, would activate the enzyme phenylethanolamine N-methyl transferase, thereby improving the synthesis of epinephrine as part of the stress-response.

Registro:

Documento: Artículo
Título:Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress
Autor:Zallocchi, M.; Matković, L.; Damasco, M.C.
Filiación:Dpto. Quím. Biológica, Pabellón 2, Ciudad Universitaria, Cd. Auton. de (1428) Buenos Aires, Argentina
Palabras clave:Acidosis; Adrenal; HSD; Stress; 11beta hydroxysteroid dehydrogenase; adrenalin; corticosterone; dehydrocorticosterone; glucocorticoid; hydrochloric acid; isoenzyme; nicotinamide adenine dinucleotide; nicotinamide adenine dinucleotide phosphate; phenylethanolamine n methyltransferase; adrenal gland; animal experiment; animal tissue; article; controlled study; enzyme activation; enzyme activity; experimental rat; feeding; homeostasis; male; measurement; microsome; nonhuman; priority journal; protein synthesis; rat; simulation; stress; thin layer chromatography
Año:2004
Volumen:82
Número:6
Página de inicio:422
Página de fin:425
DOI: http://dx.doi.org/10.1139/y04-035
Título revista:Canadian Journal of Physiology and Pharmacology
Título revista abreviado:Can. J. Physiol. Pharmacol.
ISSN:00084212
CODEN:CJPPA
CAS:11beta hydroxysteroid dehydrogenase, 9041-46-7; corticosterone, 50-22-6; dehydrocorticosterone, 72-23-1; hydrochloric acid, 7647-01-0; nicotinamide adenine dinucleotide, 53-84-9; nicotinamide adenine dinucleotide phosphate, 53-59-8; phenylethanolamine n methyltransferase, 9037-68-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v82_n6_p422_Zallocchi

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Citas:

---------- APA ----------
Zallocchi, M., Matković, L. & Damasco, M.C. (2004) . Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress. Canadian Journal of Physiology and Pharmacology, 82(6), 422-425.
http://dx.doi.org/10.1139/y04-035
---------- CHICAGO ----------
Zallocchi, M., Matković, L., Damasco, M.C. "Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress" . Canadian Journal of Physiology and Pharmacology 82, no. 6 (2004) : 422-425.
http://dx.doi.org/10.1139/y04-035
---------- MLA ----------
Zallocchi, M., Matković, L., Damasco, M.C. "Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress" . Canadian Journal of Physiology and Pharmacology, vol. 82, no. 6, 2004, pp. 422-425.
http://dx.doi.org/10.1139/y04-035
---------- VANCOUVER ----------
Zallocchi, M., Matković, L., Damasco, M.C. Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress. Can. J. Physiol. Pharmacol. 2004;82(6):422-425.
http://dx.doi.org/10.1139/y04-035