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Abstract:

We studied the effect of adrenalectomy and acute hormone replacement, using physiological doses of natural corticosteroids, on the kinetics of the Na+/H+ exchanger in brush border membrane vesicles. We collected the data using the acridine orange uptake technique. Adrenalectomized (ADX) rats presented a decreased maximal rate (V(max)) when compared with sham-operated animals (30 000 versus 41 000 fluorescent units/min, respectively). Administration of corticosterone (B) to ADX rats restored V(max) to values above control (up to 66,000 fluorescent units/min). Smaller doses of 18-OH-B led to similar results. K(m) (16 mM) remained the same for all the groups. Amiloride behaved as a pure competitive inhibitor, with a K(i) = 0.02 mM and an I50 = 98 μM (in the presence of 50 mM sodium gluconate). The presence of sodium in the external buffer, before adding the vesicles, inhibited the exchange, with an I50 = 2 mM. We observed, a significant decrease in the Na+/H+ exchanger under non-acidotic conditions in response to adrenalectomy. Acute administration of physiological doses of natural occurring corticosteroids reversed the effect.

Registro:

Documento: Artículo
Título:Acute effects of two natural corticosteroids on the kinetics of the Na+/H+ exchanger in rat renal brush-border membranes
Autor:Igarreta, P.; Calvo, J.C.; Paladini, A.; Damasco, M.C.
Filiación:PRHOM (CONICET), Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (1428) Buenos Aires, Argentina
Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Inst. de Ing. Genet. y Biol. Molec., Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
c/o Dr. Juan C. Calvo, Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:18-OH-corticosterone; Adrenalectomy; Amiloride; Corticosterone; Na+/H+ antiporter; 18 hydroxycorticosterone; adenosine triphosphatase (potassium sodium); amiloride; corticosteroid; corticosterone; adrenalectomy; animal experiment; animal tissue; article; brush border; controlled study; enzyme inhibition; fluorescence; hormone substitution; kidney proximal tubule; michaelis menten kinetics; nonhuman; priority journal; rat; 18-Hydroxycorticosterone; Acid-Base Equilibrium; Adrenalectomy; Amiloride; Animals; Anti-Inflammatory Agents; Corticosterone; Diuretics; Kidney; Kinetics; Male; Microvilli; Rats; Rats, Sprague-Dawley; Sodium; Sodium-Hydrogen Antiporter
Año:1997
Volumen:75
Número:10-11
Página de inicio:1226
Página de fin:1231
DOI: http://dx.doi.org/10.1139/cjpp-75-10-11-1226
Título revista:Canadian Journal of Physiology and Pharmacology
Título revista abreviado:Can. J. Physiol. Pharmacol.
ISSN:00084212
CODEN:CJPPA
CAS:18-Hydroxycorticosterone, 561-65-9; Amiloride, 2609-46-3; Anti-Inflammatory Agents; Corticosterone, 50-22-6; Diuretics; Sodium, 7440-23-5; Sodium-Hydrogen Antiporter
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v75_n10-11_p1226_Igarreta

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Citas:

---------- APA ----------
Igarreta, P., Calvo, J.C., Paladini, A. & Damasco, M.C. (1997) . Acute effects of two natural corticosteroids on the kinetics of the Na+/H+ exchanger in rat renal brush-border membranes. Canadian Journal of Physiology and Pharmacology, 75(10-11), 1226-1231.
http://dx.doi.org/10.1139/cjpp-75-10-11-1226
---------- CHICAGO ----------
Igarreta, P., Calvo, J.C., Paladini, A., Damasco, M.C. "Acute effects of two natural corticosteroids on the kinetics of the Na+/H+ exchanger in rat renal brush-border membranes" . Canadian Journal of Physiology and Pharmacology 75, no. 10-11 (1997) : 1226-1231.
http://dx.doi.org/10.1139/cjpp-75-10-11-1226
---------- MLA ----------
Igarreta, P., Calvo, J.C., Paladini, A., Damasco, M.C. "Acute effects of two natural corticosteroids on the kinetics of the Na+/H+ exchanger in rat renal brush-border membranes" . Canadian Journal of Physiology and Pharmacology, vol. 75, no. 10-11, 1997, pp. 1226-1231.
http://dx.doi.org/10.1139/cjpp-75-10-11-1226
---------- VANCOUVER ----------
Igarreta, P., Calvo, J.C., Paladini, A., Damasco, M.C. Acute effects of two natural corticosteroids on the kinetics of the Na+/H+ exchanger in rat renal brush-border membranes. Can. J. Physiol. Pharmacol. 1997;75(10-11):1226-1231.
http://dx.doi.org/10.1139/cjpp-75-10-11-1226