The role of amiloride-dependent sodium channels in the action of adrenal cortical steroids on urine-blood PCO2 (U-B PCO2) differences was studied in bicarbonate-infused and amiloride-treated adrenalectomized rats. U-B PCO2 was significantly reduced by amiloride in bicarbonate-infused control rats. Adrenalectomy further reduced U-B PCO2 in amiloride-treated, bicarbonate-infused rats (from 27.9 ± 1.82 mmHg in sham-operated rats to 21.3 ± 1.58 mmHg in adrenalectomized (ADX) rats) (1 mmHg = 133.322 Pa). Acute administration of corticosterone and 18-hydroxycorticosterone (18-OH-B), but not of aldosterone, caused recovery of U-B PCO2 to the level of sham-operated animals treated with amiloride. Aldosterone did not affect U-B PCO2 in the presence of amiloride (21.9 mmHg ADX group vs. 20.98 mmHg aldosterone group). Results are compatible with aldosterone affecting distal H ion secretion mostly by a sodium and potential difference dependent mechanism, while corticosterone and 18-OH-B should act by other mechanisms (e.g., increased luminal buffer level).
Documento: | Artículo |
Título: | Role of corticosteroids in distal acidification of amiloride-treated rats |
Autor: | Ansaldo, M.; Damasco, M.C.; De Lavallaz, M.S.; Lantos, C.P.; Malnic, G. |
Filiación: | Laboratorio de Esteroides, Departamento de Quimica Biologica, Fac. de Ciencias Exactas y Naturales, 1428 Buenos Aires, Argentina |
Palabras clave: | 18-hydroxycorticosterone; adrenalectomy; aldosterone; amiloride; corticos terone; corticosteroids; distal H ion secretion; 18 hydroxycorticosterone; aldosterone; amiloride; corticosterone; adrenalectomy; animal experiment; article; controlled study; intramuscular drug administration; intravenous drug administration; kidney distal tubule; male; nonhuman; priority journal; rat; urine acidification |
Año: | 1992 |
Volumen: | 70 |
Número: | 5 |
Página de inicio: | 695 |
Página de fin: | 700 |
DOI: | http://dx.doi.org/10.1139/y92-089 |
Título revista: | Canadian Journal of Physiology and Pharmacology |
Título revista abreviado: | CAN. J. PHYSIOL. PHARMACOL. |
ISSN: | 00084212 |
CODEN: | CJPPA |
CAS: | 18 hydroxycorticosterone, 561-65-9; aldosterone, 52-39-1, 6251-69-0; amiloride, 2016-88-8, 2609-46-3; corticosterone, 50-22-6 |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v70_n5_p695_Ansaldo |