The role of adrenocortical steroids in distal nephron acidification was studied in rats by measuring urine minus blood PCO2 differences (U-B PCO2) in control, sham-operated, and adrenalectomized (ADX) animals. Operations were performed 48 h before experiments. During the experiments, all rats received an infusion of 0.35-0.60 M NaHCO3, leading to urine bicarbonate concentrations in the order of 100-200 mM. Adrenalectomized rats had significantly decreased U-B PCO2 (11.9 ± 1.99 mmHg; 1 mmHg = 133.3 Pa) with respect to sham-operated rats (39.9 ± 1.26 mmHg). In another series, ADX rats received supplements of the adrenal steroids corticosterone, aldosterone, and 18-hydroxycorticosterone 100 min before the experiment. U-B PCO2 increased after hormone administration: corticosterone, 30.0 ± 2.13 mmHg; aldosterone, 26.6 ± 1.74 mmHg; 18-hydroxycorticosterone, 29.0 ± 1.60 mmHg; but none restored these values to normal. Combinations of two hormones were also used; only aldosterone + corticosterone restored U-B PCO2 to normal: 39.0 ± 1.66 mmHg. Renal phosphate excretion (but not urine phosphate levels) decreased significantly in ADX as compared with sham-operated rats. Extracellular volume was not significantly affected in ADX rats, which received ad libitum 0.9% NaCl for drinking. It is concluded that distal tubular acidification, as evaluated by U-B PCO2, is dependent on cortical steroids.
Documento: | Artículo |
Título: | Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification |
Autor: | Damasco, M.C.; Ansaldo, M.; Malnic, G. |
Filiación: | Laboratorio de Esteroides, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, 1428 Buenos Aires, Argentina |
Palabras clave: | 18 hydroxycorticosterone; aldosterone; bicarbonate; corticosterone; adrenalectomy; animal cell; animal experiment; blood gas; controlled study; kidney function; male; nonhuman; priority journal; rat; urine acidification |
Año: | 1989 |
Volumen: | 67 |
Número: | 6 |
Página de inicio: | 607 |
Página de fin: | 614 |
DOI: | http://dx.doi.org/10.1139/y89-097 |
Título revista: | Canadian Journal of Physiology and Pharmacology |
Título revista abreviado: | CAN. J. PHYSIOL. PHARMACOL. |
ISSN: | 00084212 |
CODEN: | CJPPA |
CAS: | 18 hydroxycorticosterone, 561-65-9; aldosterone, 52-39-1, 6251-69-0; bicarbonate, 144-55-8, 71-52-3; corticosterone, 50-22-6 |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v67_n6_p607_Damasco |