Autoimmune orchitis induced an increment in b2-arenoceptor populations in intact mouse spleen lymphocytes. Normal and autoimmune lymphocytes incubated with soterenol increased the mechanical response of isolated atria. Autoimmune cells were more effective than normal cells in inducing this response. Soterenol or spleen cells alone did not modify the contractility at the concentration used. Inhibitors of β2-adrenoceptors of spleen cells completely blunted the reaction between soterenol and lymphocytes, while when atria were exposed to butoxamine, mechanical activity induced by soterenol plus lymphocytes was not affected. Cell-free supernatants of lymphocytes exposed to soterenol elicited the reaction in the same way as soterenol-treated lymphocytes. Direct contact of cells with the assay organ was not necessary. Inhibitors of cyclooxygenase on lymphocytes blocked the reaction of soterenol-treated lymphocytes on atria, while inhibitors of lipoxygenase(s) completely blocked the reaction of atria exposed to soterenol-stimulated lymphocytes or supernatants. These results suggest that soterenol reacts with β2-adrenoceptors of normal and autoimmune cells. From this reaction, soluble factors are released that in turn trigger stimulation of the atrial contractility as a consequence of the release of oxidative products of the lipoxygenase(s) pathway of arachidonic acid from atria. The high activity of atria in the presence of autoimmune spleen cells is probably related to the increment in number of β2-adrenoceptors of these cells.
Documento: | Artículo |
Título: | Role of β2-adrenoceptors in the biological effect of autoimmune orchitis spleen cells |
Autor: | Borda, E.; Perez Leiros, C.; Sterin-Borda, L. |
Filiación: | Centro de Estudios Farmacologicos y de Principios Naturales (CEFAPRIN), CONICET, 1414 Buenos Aires, Argentina |
Palabras clave: | 7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid; beta 2 adrenergic receptor; butoxamine; dihydroalprenolol h 3; dithizone; indometacin; nordihydroguaiaretic acid; oxprenolol; phentolamine; practolol; propranolol; radioisotope; soterenol; unclassified drug; animal model; article; autoimmune disease; blood and hemopoietic system; drug binding; drug efficacy; drug interaction; drug receptor binding; heart; heart muscle contractility; lymphatic system; lymphocyte; male genital system; mouse; nonhuman; orchitis; pharmacokinetics; priority journal; spleen |
Año: | 1986 |
Volumen: | 64 |
Número: | 8 |
Página de inicio: | 1091 |
Página de fin: | 1095 |
DOI: | http://dx.doi.org/10.1139/y86-186 |
Título revista: | Canadian Journal of Physiology and Pharmacology |
Título revista abreviado: | CAN. J. PHYSIOL. PHARMACOL. |
ISSN: | 00084212 |
CODEN: | CJPPA |
CAS: | 7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid, 40786-08-1; butoxamine, 1937-89-9; dithizone, 60-10-6; indometacin, 53-86-1, 74252-25-8, 7681-54-1; nordihydroguaiaretic acid, 500-38-9; oxprenolol, 22972-97-0, 6452-71-7, 6452-73-9; phentolamine, 50-60-2, 73-05-2; practolol, 6673-35-4; propranolol, 13013-17-7, 318-98-9, 3506-09-0, 4199-09-1, 525-66-6; soterenol, 13642-52-9, 14816-67-2 |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v64_n8_p1091_Borda |