La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor


Uroporphyrinogen decarboxylase (UroD) (EC is an enzyme from the tetrapyrrole biosynthetic pathway, in which chlorophyll is the main final product in algae. This is the first time that a study on UroD activity has been performed in a green alga (Chlorella). We isolated and partially purified the enzyme from a Chlorella kessleri (Trebouxiophyceae, Chlorophyta) strain (Copahue, Neuquen, Argentina), and describe for the first time some of its properties. In C. kessleri, the decarboxylation of uroporphyrinogen III occurs in two stages, via 7 COOH and then 6 and 5 COOH intermediates, with the decarboxylation of the 7 COOH compound being the rate-limiting step for the reaction. Cultures in the exponential growth phase showed the highest specific activity values. The most suitable conditions to measure UroD activity in C. kessleri were as follows: 0.23-0.3 mg protein/mL, ≈6-8 μmol/L uroporphyrinogen III, and 20 min incubation time. Gel filtration chromatography and Western blot assays indicated that UroD from C. kessleri is a dimer of approximately 90 kDa formed by species of lower molecular mass, which conserves enzymatic activity. © 2007 NRC.


Documento: Artículo
Título:Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)
Autor:Juárez, A.B.; Aldonatti, C.; Vigna, M.S.; Ríos De Molina, M.C.
Filiación:Departamento de Biodiversidad Y Biología Experimental, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (C1428EHA) Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (C1428EHA) Buenos Aires, Argentina
Palabras clave:Chlorella kessleri; Chlorophyll; Chlorophyta; Porphyrins; Uroporphyrinogen decarboxylase; Bioassay; Biosynthesis; Chlorophyll; Chromatographic analysis; Molecular mass; Porphyrins; Reaction kinetics; Biosynthetic pathway; Chlorella kessleri; Chlorophyta; Decarboxylation; Enzyme kinetics; chlorophyll; uroporphyrinogen decarboxylase; alga; bioassay; decarboxylation; enzyme activity; growth rate; porphyrin; article; Chlorella; controlled study; decarboxylation; enzyme activity; enzyme purification; gel filtration; green alga; incubation time; nonhuman; priority journal; Western blotting; Chlorella; Molecular Weight; Uroporphyrinogen Decarboxylase; algae; Chlorella (Chlorellales); Chlorella (Prasiolales); Chlorella (unclassified Chlorophyceae); Chlorella (unclassified Trebouxiophyceae); Chlorophyta; Parachlorella kessleri; Trebouxiophyceae
Página de inicio:303
Página de fin:312
Título revista:Canadian Journal of Microbiology
Título revista abreviado:Can. J. Microbiol.
CAS:chlorophyll, 1406-65-1, 15611-43-5; uroporphyrinogen decarboxylase, 9024-70-8; Uroporphyrinogen Decarboxylase, EC


  • Beale, S.I., Enzymes of chlorophyll biosynthesis (1999) Photosynth. Res, 60, pp. 43-73. , doi:10.1023/A:1006297731456
  • Billi de Catabbi, S., Ríos de Molina, M.C., and San Martín de Viale, L.C. 1991. Studies on the active center of rat liver porphyrinogen carboxy-lyase in vivo effect of hexachlorobenzene. Int. J. Biochem. 23: 675-679. doi:10.1016/0020-711X(91)90037-N; Bischoff, H.W., Bold, H.C., Phycological studies IV (1963) Some soil algae from Enchanted Rock and related algal species, p. 6318. , Univ. Texas Publ, Austin, Texas
  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem, 72, pp. 248-254. , doi:10.1016/0003-2697(76)90527-3
  • Chaufan, G., Ríos de Molina, M.C., and San Martín de Viale, L.C. 2001. How does hexachlorobenzene treatment affect liver uroporphyrinogen decarboxylase? Int. J. Biochem. Cell Biol. 33: 621-630. doi:10.1016/S1357-2725(01)00034-6; Chaufan, G., Corvi, M., Armesto, A., San Martín de Viale, L.C., Luquet, C., and Ríos de Molina, M.C. 2002. Comparison between crab hepatopancreas and rat liver uroporphyrinogen decarboxylase. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 133: 251-256; De Verneuil, H., Sassa, S., Kappas, A., Effects of polychlorinated biphenyl compounds, 2, 3, 7, 8-tetrachlorodibenzo-p- dioxin, phenobarbital and iron on hepatic uroporphyrinogen decarboxylase (1983) Biochem. J, 214, pp. 145-151
  • De Verneuil, H., Sassa, S., Kappas, A., Purification and properties of uroporphyrinogen decarboxylase from human erythrocytes. A single enzyme catalysing the four sequential decarboxylations of uroporphyrinogens I and III (1983) J. Biol. Chem, 258, pp. 2454-2460
  • Elder, G.H., Tovey, J.A., Sheppard, D.M., Purification of uroporphyrinogen decarboxylase from human erythrocytes. Immunochemical evidence for a single protein with decarboxylase activity in human erythrocytes and liver (1983) Biochem. J, 215, pp. 45-55
  • Felix, F., Brouillet, N., Purification and properties of uroporphyrinogen decarboxylase from Saccharomyces cerevisiae. Yeast uroporphyrinogen decarboxylase (1990) Eur. J. Biochem, 188, pp. 393-403. , doi:10.1111/j.1432-1033.1990.tb15416.x
  • García, R.C., San Martín de Viale, L.C., Tomio, J.M., and Grinstein, M. 1973. Porphyrin biosynthesis. Porphyrinogen carboxylyase from avian erythrocytes. Further properties. Biochim. Biophys. Acta, 309: 203-210; Grimm, B., Novel insights in the control of tetrapyrrole metabolism of higher plants (1998) Curr. Opin. Plant Biol, 1, pp. 245-250. , doi:10.1016/S1369-5266(98)80112-X
  • Jackson, A.H., Sancovich, H.A., Ferramola, A.M., Evans, N., Games, D.E., Matlin, S.A., Elder, G.H., Smith, S.G., Macrocyclic intermediates in the biosynthesis of porphyrins (1976) Phil. Trans. R. Soc. Lond. B, 273, pp. 191-206
  • Jones, R.M., Jordan, P.M., Purification and properties of the uroporphyrinogen decarboxylase from Rhodobacter sphaeroides (1993) Biochem. J, 293, pp. 703-712
  • Juárez, A.B., Vélez, C.G., Sobre la presencia de Chlorella kessleri (Chlorococcales, Chlorophyta) en aguas del complejo termal Copahue (prov. del Neuquén, Argentina) (1993) Bol. Soc. Argent. Bot, 29, pp. 105-107
  • Juknat, A.A., Seubert, A., Seubert, S., Ippen, H., Studies on uroporphyrinogen decarboxylase of etiolated Euglena gracilis Z (1989) Eur. J. Biochem, 179, pp. 423-428. , doi:10.1111/j.1432-1033.1989.tb14570.x
  • Kappas, A., Sassa, S., Galbraith, R.A., Nordmann, Y., The porphyries (1995) The metabolic and molecular bases of inherited disease, pp. 2103-2160. , Edited by C.R. Scriver, A.L Beaudet, W.S. Sly, and D. Valle. McGraw-Hill Inc, New York. pp
  • Kawanishi, S., Seki, Y., Sano, S., Uroporphyrinogen decarboxylase. Purification, properties, and inhibition by polychlorinated biphenyl isomers (1983) J. Biol. Chem, 258, pp. 4285-4292
  • Koopmann, G.E., Juknat deGeralnik, A., and Del C.-Beatlle, A.M. 1986. Porphyrin biosynthesis in Rhodopseudomonas palustris. V. Purification of porphyrinogen decarboxylase and some unusual properties. Int. J. Biochem. 18: 935-944. doi:10.1016/0020-711X(86)90075-3; Marker, A.F.H., Nusch, E.A., Rai, H., and Rienemann, B. 1980. The measurement of photosynthetic pigments in freshwater and standardization of methods: conclusions and recommendations. Arch. Hydrobiol./Beih. Ergebn. Limnol., Stuttgart, 14: 91-106; Martins, B.M., Grimm, B., Mock, H.P., Huber, R., Messerschmidt, A., Crystal structure and substrate binding modeling of the uroporphyrinogen-III decarboxylase from Nicotiana tabacum. Implications for the catalytic mechanism (2001) J. Biol. Chem, 276, pp. 44108-44116. , doi:10.1074/jbc.M104759200
  • Mock, H.P., Trainotti, L., Kruse, E., Grimm, B., Isolation, sequencing and expression of cDNA sequences encoding uroporphyrinogen decarboxylase from tobacco and barley (1995) Plant Mol. Biol, 28, pp. 245-256. , doi:10.1007/BF00020244
  • Ríos de Molina, M.C., Wainstok de Calmanovici, R., and San Martín de Viale, L.C. 1987. Liver porphyrinogen carboxylyase in hexachlorobenzene porphyric rats. Studies with intermediate porphyrinogens of series III and with uroporphyrinogen I. Int. J. Biochem. 19: 365-372. doi:10.1016/0020-711X(87)90010-3; Ríos de Molina, M.C., Chaufan, G., Iglesias, S., Billi de Catabbi, S., and San Martín de Viale, L.C. 1996. Porphyrinogen carboxylyase. Studies on the existence of isoenzymes. Acta Physiol. Pharmacol. Ther. Latinoam. 46: 265-275; Ríos de Molina, M.C., Corvi, M., Chaufan, G., Guidi, S., and San Martín de Viale, L.C. 1999. Detection of four isoforms for rat liver uroporphyrinogen decarboxylase. Anales Asoc. Quim. Arg. 87: 121-125; Seki, Y., Kawanishi, S., Sano, S., Uroporphyrinogen decarboxylase purification from chicken erythrocytes (1986) Methods Enzymol, 123, pp. 415-421
  • Stewart, W.D.P., (1974) Algal physiology and biochemistry, , Blackwell Scientific Publications, Oxford, London
  • Straka, J.G., Kushner, J.P., Purification and characterization of bovine hepatic uroporphyrinogen decarboxylase (1983) Biochemistry, 22, pp. 4664-4672. , doi:10.1021/bi00289a009
  • Vavilin, D.V., Vermaas, W.F.J., Regulation of the tetrapyrrole biosynthetic pathway leading to heme and chlorophyll in plants and cyanobacteria (2002) Physiol. Plant, 115, pp. 9-24. , doi:10.1034/j.1399-3054.2002. 1150102.x
  • Von Wettstein, D., Gough, S., Kannangara, G., Chlorophyll biosynthesis (1995) Plant Cell, 7, pp. 1039-1057. , doi:10.1105/tpc.7.7.1039
  • Whitby, F.G., Phillips, J.D., Kushner, J.P., Hill, C.P., Crystal structure of human uroporphyrinogen decarboxylase (1998) EMBO J, 17, pp. 2463-2471. , doi:10.1093/emboj/17.9.2463


---------- APA ----------
Juárez, A.B., Aldonatti, C., Vigna, M.S. & Ríos De Molina, M.C. (2007) . Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta). Canadian Journal of Microbiology, 53(2), 303-312.
---------- CHICAGO ----------
Juárez, A.B., Aldonatti, C., Vigna, M.S., Ríos De Molina, M.C. "Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)" . Canadian Journal of Microbiology 53, no. 2 (2007) : 303-312.
---------- MLA ----------
Juárez, A.B., Aldonatti, C., Vigna, M.S., Ríos De Molina, M.C. "Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)" . Canadian Journal of Microbiology, vol. 53, no. 2, 2007, pp. 303-312.
---------- VANCOUVER ----------
Juárez, A.B., Aldonatti, C., Vigna, M.S., Ríos De Molina, M.C. Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta). Can. J. Microbiol. 2007;53(2):303-312.