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Abstract:

Uroporphyrinogen decarboxylase (UroD) (EC 4.1.1.37) is an enzyme from the tetrapyrrole biosynthetic pathway, in which chlorophyll is the main final product in algae. This is the first time that a study on UroD activity has been performed in a green alga (Chlorella). We isolated and partially purified the enzyme from a Chlorella kessleri (Trebouxiophyceae, Chlorophyta) strain (Copahue, Neuquen, Argentina), and describe for the first time some of its properties. In C. kessleri, the decarboxylation of uroporphyrinogen III occurs in two stages, via 7 COOH and then 6 and 5 COOH intermediates, with the decarboxylation of the 7 COOH compound being the rate-limiting step for the reaction. Cultures in the exponential growth phase showed the highest specific activity values. The most suitable conditions to measure UroD activity in C. kessleri were as follows: 0.23-0.3 mg protein/mL, ≈6-8 μmol/L uroporphyrinogen III, and 20 min incubation time. Gel filtration chromatography and Western blot assays indicated that UroD from C. kessleri is a dimer of approximately 90 kDa formed by species of lower molecular mass, which conserves enzymatic activity. © 2007 NRC.

Registro:

Documento: Artículo
Título:Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)
Autor:Juárez, A.B.; Aldonatti, C.; Vigna, M.S.; Ríos De Molina, M.C.
Filiación:Departamento de Biodiversidad Y Biología Experimental, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (C1428EHA) Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (C1428EHA) Buenos Aires, Argentina
Palabras clave:Chlorella kessleri; Chlorophyll; Chlorophyta; Porphyrins; Uroporphyrinogen decarboxylase; Bioassay; Biosynthesis; Chlorophyll; Chromatographic analysis; Molecular mass; Porphyrins; Reaction kinetics; Biosynthetic pathway; Chlorella kessleri; Chlorophyta; Decarboxylation; Enzyme kinetics; chlorophyll; uroporphyrinogen decarboxylase; alga; bioassay; decarboxylation; enzyme activity; growth rate; porphyrin; article; Chlorella; controlled study; decarboxylation; enzyme activity; enzyme purification; gel filtration; green alga; incubation time; nonhuman; priority journal; Western blotting; Chlorella; Molecular Weight; Uroporphyrinogen Decarboxylase; algae; Chlorella (Chlorellales); Chlorella (Prasiolales); Chlorella (unclassified Chlorophyceae); Chlorella (unclassified Trebouxiophyceae); Chlorophyta; Parachlorella kessleri; Trebouxiophyceae
Año:2007
Volumen:53
Número:2
Página de inicio:303
Página de fin:312
DOI: http://dx.doi.org/10.1139/W06-117
Título revista:Canadian Journal of Microbiology
Título revista abreviado:Can. J. Microbiol.
ISSN:00084166
CODEN:CJMIA
CAS:chlorophyll, 1406-65-1, 15611-43-5; uroporphyrinogen decarboxylase, 9024-70-8; Uroporphyrinogen Decarboxylase, EC 4.1.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084166_v53_n2_p303_Juarez

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Citas:

---------- APA ----------
Juárez, A.B., Aldonatti, C., Vigna, M.S. & Ríos De Molina, M.C. (2007) . Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta). Canadian Journal of Microbiology, 53(2), 303-312.
http://dx.doi.org/10.1139/W06-117
---------- CHICAGO ----------
Juárez, A.B., Aldonatti, C., Vigna, M.S., Ríos De Molina, M.C. "Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)" . Canadian Journal of Microbiology 53, no. 2 (2007) : 303-312.
http://dx.doi.org/10.1139/W06-117
---------- MLA ----------
Juárez, A.B., Aldonatti, C., Vigna, M.S., Ríos De Molina, M.C. "Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)" . Canadian Journal of Microbiology, vol. 53, no. 2, 2007, pp. 303-312.
http://dx.doi.org/10.1139/W06-117
---------- VANCOUVER ----------
Juárez, A.B., Aldonatti, C., Vigna, M.S., Ríos De Molina, M.C. Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta). Can. J. Microbiol. 2007;53(2):303-312.
http://dx.doi.org/10.1139/W06-117