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Abstract:

The robber fly Mallophora ruficauda is one of the most important apicultural pests in the Pampas region of Argentina. This species is a parasitoid of scarab beetle larvae. Females lay eggs away from the host, and the larvae perform active search behaviour toward Cyclocephala signaticollis third instar larvae, parasitoid's preferred host. This behaviour is mediated by host-related chemical cues produced in hosts' fermentation chamber. Also, C. signaticollis larvae are attracted to fermentation chamber extracts. As scarab larvae have microbe-rich fermentation chamber, it has been suggested that microorganisms could be involved in the production of these semiochemicals. The aims of this work were first to ascertain the presence of microorganisms in the fermentation chamber of C. signaticollis larvae and second to determine the role of microorganisms in the orientation response of parasitoid and host larvae. We found that microorganisms-free C. signaticollis larvae showed deterioration in their development and did not produce the attractive semiochemicals. Therefore, we isolated fermentation chamber microorganisms of host larvae by means of different cultures media, and then, assayed different microorganisms' stimuli by binary choice tests. We were able to isolate microorganisms and determine that M. ruficauda larvae are attracted to semiochemicals from protein degradation in the fermentation chamber. However, C. signaticollis larvae were not attracted to any semiochemicals associated with microorganisms' activity in the fermentation chamber. Although we were unable to elucidate the exact role of gut microorganisms in host behaviour, we discuss their relevance in parasitoid host-seeking behaviour and host conspecific interaction in M. ruficauda-C. signaticollis system. Copyright © Cambridge University Press 2015.

Registro:

Documento: Artículo
Título:Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda
Autor:Groba, H.F.; Castelo, M.K.
Filiación:Grupo de Investigación en Ecofisiología de Parasitoides (GIEP), Departamento de Ecología, Genética y Evolución- Instituto IEGEBA (CONICET - UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, Pabellón II, Ciudad de Buenos Aires, C1428EHA, Argentina
Palabras clave:Asilidae; host location; microorganisms; parasitoid; Scarabaeidae; semiochemicals; apiculture; bee; beetle; bioassay; chemical cue; entomology; fly; host location; host-parasitoid interaction; larva; microorganism; parasitoid; pest species; protein; semiochemical; Argentina; Pampas; Asilidae; Mallophora ruficauda; Scarabaeidae; animal; animal behavior; association; beetle; Diptera; growth, development and aging; host parasite interaction; intestine flora; larva; microbiology; orientation; parasitology; physiology; Animals; Beetles; Behavior, Animal; Cues; Diptera; Gastrointestinal Microbiome; Host-Parasite Interactions; Larva; Orientation
Año:2016
Volumen:106
Número:1
Página de inicio:81
Página de fin:90
DOI: http://dx.doi.org/10.1017/S0007485315000838
Título revista:Bulletin of Entomological Research
Título revista abreviado:Bull. Entomol. Res.
ISSN:00074853
CODEN:BEREA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00074853_v106_n1_p81_Groba

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Citas:

---------- APA ----------
Groba, H.F. & Castelo, M.K. (2016) . Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda. Bulletin of Entomological Research, 106(1), 81-90.
http://dx.doi.org/10.1017/S0007485315000838
---------- CHICAGO ----------
Groba, H.F., Castelo, M.K. "Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda" . Bulletin of Entomological Research 106, no. 1 (2016) : 81-90.
http://dx.doi.org/10.1017/S0007485315000838
---------- MLA ----------
Groba, H.F., Castelo, M.K. "Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda" . Bulletin of Entomological Research, vol. 106, no. 1, 2016, pp. 81-90.
http://dx.doi.org/10.1017/S0007485315000838
---------- VANCOUVER ----------
Groba, H.F., Castelo, M.K. Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda. Bull. Entomol. Res. 2016;106(1):81-90.
http://dx.doi.org/10.1017/S0007485315000838