Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Normal human lymphocytes (L) (8 × 105 ml−1) incubated with methoxamine (Me) (1 × 10−7 M) (Me‐L) triggered the mchanical response of the isolated vas deferens of the rat. L or Me alone did not modify this contractile activity at the concentrations cited above. Me alone (10−6 to 10−3 M) increased the tension of the vas. In the presence of L (8 × 105 ml−1) the dose‐response curve to Me shifted to the left and the efficacy of Me was enhanced. Inhibitors of α1‐adrenoceptors completely blocked the reaction between Me and L while drugs that block α1 and α2‐adrenoceptors reduced the reaction between Me‐L and the vas deferens. Direct contact of Me‐L with the assay organ was not necessary. Cell‐free supernatants of L exposed to Me (Me‐L supernatants) elicited the reaction in the same way as Me‐L. This effect required the continuous presence of Me since dialyzed Me‐L supernatants were inactive. Inhibitors of lipoxygenase(s) completely blocked the positive inotropic effect of Me‐L or of Me‐L supernatants. Inhibitors of cyclo‐oxygenase potentiated this effect. These results suggest that Me reacts with α1‐adrenoceptors of L. From this reaction, soluble factors are released that potentiate the α‐adrenoceptor stimulatory effect of Me on the vas deferens as a consequence of the release of oxidative products of the lipoxygenase/s pathway of arachidonic acid. 1984 British Pharmacological Society

Registro:

Documento: Artículo
Título:α‐Adrenoceptor stimulated lymphocytes trigger the mechanical response of vas deferens: participation of arachidonic acid metabolites
Autor:Borda, E.S.; de Bracco, M.M.; Cangiani, S.; Finiasz, M.; Sterin‐Borda, L.
Filiación:Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPREN), CONICET and Inmunologia de la Academia Nacional de Medicine, Serrano 665/69, Buenos Aires, 1414, Argentina
Palabras clave:7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid; acetylsalicylic acid; alpha adrenergic receptor; arachidonic acid; indometacin; lipoxygenase inhibitor; methoxamine; nordihydroguaiaretic acid; phentolamine; prazosin; prostaglandin synthase inhibitor; yohimbine; animal cell; blood and hemopoietic system; drug efficacy; human; human cell; lymphatic system; lymphocyte; male genital system; muscle; nonhuman; normal human; rat; smooth muscle contractility; vas deferens; Adrenergic alpha-Agonists; Adrenergic alpha-Antagonists; Animals; Arachidonic Acid; Arachidonic Acids; Humans; Lipoxygenase Inhibitors; Lymphocytes; Male; Methoxamine; Muscle Contraction; Muscle, Smooth; Rats; Time Factors; Vas Deferens
Año:1984
Volumen:82
Número:4
Página de inicio:863
Página de fin:870
DOI: http://dx.doi.org/10.1111/j.1476-5381.1984.tb16483.x
Título revista:British Journal of Pharmacology
Título revista abreviado:Br. J. Pharmacol.
ISSN:00071188
CAS:7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid, 40786-08-1; acetylsalicylic acid, 493-53-8, 50-78-2, 53663-74-4, 53664-49-6, 63781-77-1; arachidonic acid, 506-32-1, 6610-25-9, 7771-44-0; indometacin, 53-86-1, 74252-25-8, 7681-54-1; methoxamine, 390-28-3, 61-16-5; nordihydroguaiaretic acid, 500-38-9; phentolamine, 50-60-2, 73-05-2; prazosin, 19216-56-9, 19237-84-4; yohimbine, 146-48-5, 65-19-0; Adrenergic alpha-Agonists; Adrenergic alpha-Antagonists; Arachidonic Acid, 506-32-1; Arachidonic Acids; Lipoxygenase Inhibitors; Methoxamine, 390-28-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00071188_v82_n4_p863_Borda

Referencias:

  • BIDART, J.M., MOTTE, P.H., ASSICOT, M., BOHUON, C., BELLET, D., Catechol‐O‐methylatransferase activity and aminergic binding sites distribution in human peripheral blood lymphocyte subpopulations. (1983) Clin. Immol. Immunopat., 26, pp. 1-9
  • BORDA, E.S., AGOSTINI, M.C., GIMENO, M.F., GIMENO, A.L., Castration alters the stimulatory and inhibitory adrenergic influences on isolated rat vas deferens. (1981) Pharmac. Res. Comm., 13, pp. 981-995
  • BORDA, E.S., BRACCO, M.M., FINIASZ, M., STERINBORDA, L., Lymphocytes sensitize rat isolated atria to the inotropic and chronotropic effects of sodium arachidonate. (1984) Br. J. Pharmac., 81, pp. 75-83
  • BOYUM, A., Separation of leukocytes from blood and bone marrow. (1968) Scand. J. lab. Invest., 21, pp. 77-89
  • BURKA, J.F., PATERSON, A.M., The effects of SRS‐A and histamine antagonists on antigen‐induced contraction of guinea pig trachea. (1981) Eur. J. Pharmac., 70, pp. 489-499
  • CHISARI, F.V., EDGINGTON, T.S., Human T lymphocyte E‐rosette function. A process modulated by intracellular cyclic AMP. (1976) J. exp. Med., 140, pp. 1122-1126
  • EXTON, J.H., Molecular mechanisms involved in alpha adrenergic responses. (1981) Mol. Cell. Endocrinol., 23, pp. 233-264
  • FARESE, R.V., The phosphatide‐phosphainositide cycle: an intracellular messenger system in the action of hormones and neurotransmitters. (1983) Metabolism, 32, pp. 628-641
  • HAMBERG, M., On the formation of TXB2 and 12 L‐hydroxy‐5, 8, 10, 14‐eicosatetraenoic in tissues from guinea pig. (1976) Biochim. biophys. Acta, 431, pp. 651-654
  • HAMBERG, M., SAMUELSSON, B., Prostaglandin endoperoxides. Novel transformation of arachidonic acid in human platelets. (1974) Proc natn. acad. Sci. U.S.A., 71, pp. 5400-5403
  • HENRTKSEN, O., FREY, J.R., Control of expression of interleukin 2 activity. (1982) Cell. Immunol., 73, pp. 106-114
  • JONES, L.M., MICHELL, R.H., Stimulus‐response coupling at alpha‐adrenergic receptors. (1978) Biochem. Soc. Trans., 6, pp. 673-688
  • KELLY, J.P., JOHNSON, M.C., PARKER, C.W., Effect of inhibitors of arachidonic acid metabolite on mitagenesis in human lymphocytes: possible role of thromboxanes and products of the lipoxygenase pathway. (1979) J. Immunol., 122, pp. 1563-1571
  • KELLY, J.P., PARKER, C.W., Effects of arachidonic acid and other fatty acids on mitogenesis in human lymphocytes. (1979) J. Immunol., 122, pp. 1556-1562
  • KRAWIETZ, W., WERDAN, K., SCHOBER, M., ERDMANN, E., RINDFLEISCH, G., HANNIG, K., Different number of beta‐adrenoceptors in human lymphocyte subpopulations. (1982) Biochem. Pharmac., 31, pp. 133-136
  • LANGER, S.Z., Presynaptic regulation of the release of catecholamines. (1981) Pharmac. Rev., 32, pp. 337-355
  • LAPPIN, D., WHALEY, K., Adrenergic receptors on monocytes modulate complement component synsthesis. (1982) Clin. exp. Immunol., 47, pp. 606-612
  • MICHELL, R.H., Inositol phospholipids and cell surface receptor function. (1975) Biochim. biophys. Acta, 415, pp. 81-147
  • MICHELL, R.H., KIRK, C.J., Why is phosphatidylinositol degraded in response to stimulation of certain receptors. (1981) Trends Pharmac. Sci., 2, pp. 86-89
  • PARKER, C.W., KELLY, J.P., FALENHEIM, S.F., HUBER, M.G., Release of arachidonic acid from human lymphocytes in response to mitogenic lectins. (1979) J. exp. Med., 149, pp. 1487-1503
  • PARKER, C.W., STENSON, W.F., HUBER, M.G., KELLY, J.P., Formation of thromboxane B2 and hydroxy‐arachidonic acid in purified human lymphocytes in the presence and absence of PHA. (1979) J. Immunol., 122, pp. 1572-1578
  • PISTORIOUS, E.K., AXELROD, B., Iron, an esential component of lipoxygenase. (1974) J. biol. Chem., 249, pp. 3183-3186
  • SLESS, F., PARROTT, D.M.V., Evidence for control of complement receptor rosette‐forming cells by alpha and beta adrenergic agonists. (1977) Nature, 266, pp. 633-635
  • STERIN‐BORDA, L., BORDA, E.S., FINK, S., BRACCO, M.M., Effect of phytohemagglutinin‐stimulated human lymphocytes on isolated rat atria. Participation of lipoxgenase products of arachidonic acid metabolites. (1983) Naunyn-Schmiedeberg's Archives of Pharmacology, 324, pp. 58-63
  • VAN ROSSUM, J.M., Cumulative dose‐response curves. II. Technique for the making of dose‐response curves in isolated organs and the evaluation of drug parameters. (1963) Archs. int. Pharmacodyn. Thér., 143, pp. 299-319
  • VANE, J.R., Inhibition of prostaglandin synsthesis as a mechanism of action for aspirin‐like durgs. (1971) Nature, New Biol., 251, pp. 232-235

Citas:

---------- APA ----------
Borda, E.S., de Bracco, M.M., Cangiani, S., Finiasz, M. & Sterin‐Borda, L. (1984) . α‐Adrenoceptor stimulated lymphocytes trigger the mechanical response of vas deferens: participation of arachidonic acid metabolites. British Journal of Pharmacology, 82(4), 863-870.
http://dx.doi.org/10.1111/j.1476-5381.1984.tb16483.x
---------- CHICAGO ----------
Borda, E.S., de Bracco, M.M., Cangiani, S., Finiasz, M., Sterin‐Borda, L. "α‐Adrenoceptor stimulated lymphocytes trigger the mechanical response of vas deferens: participation of arachidonic acid metabolites" . British Journal of Pharmacology 82, no. 4 (1984) : 863-870.
http://dx.doi.org/10.1111/j.1476-5381.1984.tb16483.x
---------- MLA ----------
Borda, E.S., de Bracco, M.M., Cangiani, S., Finiasz, M., Sterin‐Borda, L. "α‐Adrenoceptor stimulated lymphocytes trigger the mechanical response of vas deferens: participation of arachidonic acid metabolites" . British Journal of Pharmacology, vol. 82, no. 4, 1984, pp. 863-870.
http://dx.doi.org/10.1111/j.1476-5381.1984.tb16483.x
---------- VANCOUVER ----------
Borda, E.S., de Bracco, M.M., Cangiani, S., Finiasz, M., Sterin‐Borda, L. α‐Adrenoceptor stimulated lymphocytes trigger the mechanical response of vas deferens: participation of arachidonic acid metabolites. Br. J. Pharmacol. 1984;82(4):863-870.
http://dx.doi.org/10.1111/j.1476-5381.1984.tb16483.x