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Abstract:

Chronic lymphocytic leukaemia (CLL) is characterized by the accumulation of long-lived B lymphocytes blocked in G0/1 by impaired apoptosis. As insulin-like growth factor-I (IGF-I) is known for its antiapoptotic effects in different cell types, we investigated whether IGF-I and its receptor (IGF-IR) participate in autocrine/paracrine loops affecting the survival of CLL cells. IGF-IR protein and mRNA was present in CLL cells in 44% and 59% of patients respectively. IGF-IR expression in CLL patients was positively correlated with the expression of the antiapoptotic protein Bcl-2 and was involved in CLL cell survival in vitro. Serum IGF-I was elevated in CLL patients, but growth hormone (GH) was normal. CLL cells expressed IGF-I mRNA and secreted the growth factor in vitro. Therefore, local production of IGF-I can account for the increased levels of serum IGF-I, independently of GH, and may be related to autocrine/paracrine control of lymphocyte survival acting at IGF-IR. This is the first demonstration of IGF-IR expression in a subgroup of CLL patients and of its antiapoptotic effects in vitro, highlighting the importance of this growth factor receptor as a possible therapeutic target in CLL. © 2005 Blackwell Publishing Ltd.

Registro:

Documento: Artículo
Título:Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia
Autor:Schillaci, R.; Galeano, A.; Becu-Villalobos, D.; Spinelli, O.; Sapia, S.; Bezares, R.F.
Filiación:Instituto de Biología y Medicina Experimental, CONICET, Buenos Aires, Argentina
Laboratorio de Patología Sanatoria Mater Dei, Buenos Aires, Argentina
Hospital Municipal Teodora Alvarez, Buenos Aires, Argentina
Instituto de Biologia y Medicina Experimental, CONICET, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:B cells; Chronic lymphocytic leukaemia (CLL); CLL apoptosis; CLL growth factors; growth hormone; messenger RNA; protein bcl 2; somatomedin C; somatomedin C receptor; adult; aged; apoptosis; article; autocrine effect; B lymphocyte; cell cycle; cell survival; chronic lymphatic leukemia; clinical article; controlled study; female; flow cytometry; human; human cell; immunophenotyping; male; paracrine signaling; priority journal; protein expression; reverse transcription polymerase chain reaction; statistical analysis; Aged; Aged, 80 and over; Analysis of Variance; Apoptosis; Autocrine Communication; B-Lymphocytes; Case-Control Studies; Culture Media, Conditioned; Female; Flow Cytometry; Growth Hormone; Humans; Insulin-Like Growth Factor I; Leukemia, Lymphocytic, Chronic; Male; Middle Aged; Paracrine Communication; Receptor, IGF Type 1; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Tumor Cells, Cultured
Año:2005
Volumen:130
Número:1
Página de inicio:58
Página de fin:66
DOI: http://dx.doi.org/10.1111/j.1365-2141.2005.05579.x
Título revista:British Journal of Haematology
Título revista abreviado:Br. J. Haematol.
ISSN:00071048
CODEN:BJHEA
CAS:growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; protein bcl 2, 219306-68-0; somatomedin C, 67763-96-6; Culture Media, Conditioned; Growth Hormone, 9002-72-6; Insulin-Like Growth Factor I, 67763-96-6; Receptor, IGF Type 1, EC 2.7.1.112; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00071048_v130_n1_p58_Schillaci

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Citas:

---------- APA ----------
Schillaci, R., Galeano, A., Becu-Villalobos, D., Spinelli, O., Sapia, S. & Bezares, R.F. (2005) . Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia. British Journal of Haematology, 130(1), 58-66.
http://dx.doi.org/10.1111/j.1365-2141.2005.05579.x
---------- CHICAGO ----------
Schillaci, R., Galeano, A., Becu-Villalobos, D., Spinelli, O., Sapia, S., Bezares, R.F. "Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia" . British Journal of Haematology 130, no. 1 (2005) : 58-66.
http://dx.doi.org/10.1111/j.1365-2141.2005.05579.x
---------- MLA ----------
Schillaci, R., Galeano, A., Becu-Villalobos, D., Spinelli, O., Sapia, S., Bezares, R.F. "Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia" . British Journal of Haematology, vol. 130, no. 1, 2005, pp. 58-66.
http://dx.doi.org/10.1111/j.1365-2141.2005.05579.x
---------- VANCOUVER ----------
Schillaci, R., Galeano, A., Becu-Villalobos, D., Spinelli, O., Sapia, S., Bezares, R.F. Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia. Br. J. Haematol. 2005;130(1):58-66.
http://dx.doi.org/10.1111/j.1365-2141.2005.05579.x