Artículo

Este artículo es de Acceso Abierto y puede ser descargado en su versión final desde nuestro repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The role of oxidative stress in prostate cancer has been increasingly recognised. Acute and chronic inflammations generate reactive oxygen species that result in damage to cellular structures. Haeme oxygenase-1 (HO-1) has cytoprotective effects against oxidative damage. We hypothesise that modulation of HO-1 expression may be involved in the process of prostate carcinogenesis and prostate cancer progression. We thus studied HO-1 expression and localisation in 85 samples of organ-confined primary prostate cancer obtained via radical prostatectomy (Gleason grades 4-9) and in 39 specimens of benign prostatic hyperplasia (BPH). We assessed HO-1 expression by immunohistochemical staining. No significant difference was observed in the cytoplasmic positive reactivity among tumours (84%), non-neoplastic surrounding parenchyma (89%), or BPH samples (87%) (P=0.53). Haeme oxygenase-1 immunostaining was detected in the nuclei of prostate cancer cells in 55 of 85 (65%) patients but less often in non-neoplastic surrounding parenchyma (30 of 85, 35%) or in BPH (9 of 39, 23%) (P<0.0001). Immunocytochemical and western blot analysis showed HO-1 only in the cytoplasmic compartment of PC3 and LNCaP prostate cancer cell lines. Treatment with hemin, a well-known specific inducer of HO-1, led to clear nuclear localisation of HO-1 in both cell lines and highly induced HO-1 expression in both cellular compartments. These findings have demonstrated, for the first time, that HO-1 expression and nuclear localisation can define a new subgroup of prostate cancer primary tumours and that the modulation of HO-1 expression and its nuclear translocation could represent new avenues for therapy. © 2007 Cancer Research UK.

Registro:

Documento: Artículo
Título:Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer
Autor:Sacca, P.; Meiss, R.; Casas, G.; Mazza, O.; Calvo, J.C.; Navone, N.; Vazquez, E.
Filiación:Instituto de Biología Y Medicina Experimental, CONICET, Vuelta de Obligado 2490, Buenos Aires 1428, Argentina
Departamento de Patología, Instituto de Estudios Oncológicos, Academia Nacional de Medicina, Pacheco de Melo 3081, Buenos Aires 1425, Argentina
Departamento de Patología, Hospital Alemán, Buenos Aires, Argentina
Servicio Urología, Hospital de Clínicas, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Pabellón II, Buenos Aires 1428, Argentina
Department of Genitourinary Medical Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, United States
Intendente Güiraldes 2160, Ciudad Universitaria, Pab. II, Buenos Aires 1428, Argentina
Palabras clave:Haeme oxygenase-1; Nuclear localisation; Oxidative stress; Prostate cancer; heme oxygenase 1; hemin; adult; aged; article; cancer cell culture; cancer growth; carcinogenesis; cell compartmentalization; cell nucleus; cell protection; controlled study; disease association; disease course; enzyme localization; enzyme regulation; Gleason score; human; human cell; human tissue; immunohistochemistry; male; oxidative stress; priority journal; prostate cancer; prostate hypertrophy; prostatectomy; protein expression; Western blotting; Active Transport, Cell Nucleus; Adult; Aged; Aged, 80 and over; Cell Nucleus; Heme Oxygenase-1; Hemin; Humans; Male; Middle Aged; Prostatic Hyperplasia; Prostatic Neoplasms; Tumor Cells, Cultured
Año:2007
Volumen:97
Número:12
Página de inicio:1683
Página de fin:1689
DOI: http://dx.doi.org/10.1038/sj.bjc.6604081
Título revista:British Journal of Cancer
Título revista abreviado:Br. J. Cancer
ISSN:00070920
CODEN:BJCAA
CAS:hemin, 16009-13-5; Heme Oxygenase-1, EC 1.14.99.3; Hemin, 16009-13-5
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00070920_v97_n12_p1683_Sacca.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v97_n12_p1683_Sacca

Referencias:

  • Alam J, Igarashi K, Immenschuh S, Shibahara S, Tyrrell RM (2004) Regulation of heme oxygenase-1 gene transcription: recent advances and highlights from the International Conference (Uppsala, 2003) on Heme Oxygenase. Antioxid Redox Signal 6: 924-933; Aydin, A., Arsova-Sarafinovska, Z., Sayal, A., Eken, A., Erdem, O., Erten, K., Ozgok, Y., Dimovski, A., Oxidative stress and antioxidant status in non-metastatic prostate cancer and benign prostatic hyperplasia (2006) Clin Biochem, 39, pp. 176-179
  • Berberat, P.O., Dambrauskas, Z., Gulbinas, A., Giese, T., Giese, N., Kunzli, B., Autschbach, F., Friess, H., Inhibition of heme oxygenase-1 increases responsiveness of pancreatic cancer cells to anticancer treatment (2005) Clin Cancer Res, 11, pp. 3790-3798
  • Busserolles, J., Megias, J., Terencio, M.C., Alcaraz, M.J., Heme oxygenase-1 inhibits apoptosis in Caco-2 cells via activation of Akt pathway (2006) Int J Biochem Cell Biol, 38, pp. 1510-1517
  • Caballero, F., Meiss, R., Gimenez, A., Batlle, A., Vazquez, E., Immunohistochemical analysis of heme oxygenase-1 in preneoplastic and neoplastic lesions during chemical hepatocarcinogenesis (2004) Int J Exp Pathol, 85, pp. 213-222
  • Calabrese, V., Maines, M.D., Antiaging medicine: Antioxidants and aging (2006) Antioxid Redox Signal, 8, pp. 362-364
  • Chen, X., Ding, Y.W., Yang, G., Bondoc, F., Lee, M.J., Yang, C.S., Oxidative damage in an esophageal adenocarcinoma model with rats (2000) Carcinogenesis, 21, pp. 257-263
  • De Marzo, A.M., Meeker, A.K., Zha, S., Luo, J., Nakayama, M., Platz, E.A., Isaacs, W.B., Nelson, W.G., Human prostate cancer precursors and pathobiology (2003) Urology, 62, pp. 55-62
  • Dong, Z., Lavrovsky, Y., Venkatachalam, M.A., Roy, A.K., Heme oxygenase-1 in tissue pathology: The Yin and Yang (2000) Am J Pathol, 156, pp. 1485-1488
  • Dulak, J., Loboda, A., Zagorska, A., Jozkowicz, A., Complex role of heme oxygenase-1 in angiogenesis (2004) Antioxid Redox Signal, 6, pp. 858-866
  • Esposito, F., Cuccovillo, F., Morra, F., Russo, T., Cimino, F., DNA binding activity of the glucocorticoid receptor is sensitive to redox changes in intact cells (1995) Biochim Biophys Acta, 1260, pp. 308-314
  • Fang, J., Sawa, T., Akaike, T., Akuta, T., Sahoo, S.K., Khaled, G., Hamada, A., Maeda, H., In vivo antitumor activity of pegylated zinc protoporphyrin: Targeted inhibition of heme oxygenase in solid tumor (2003) Cancer Res, 63, pp. 3567-3574
  • Fleshner, N., Bagnell, P.S., Klotz, L., Venkateswaran, V., Dietary fat and prostate cancer (2004) J Urol, 171, pp. S19-S24
  • Giordano, A., Nisoli, E., Tonello, C., Cancello, R., Carruba, M.O., Cinti, S., Expression and distribution of heme oxygenase-1 and -2 in rat brown adipose tissue: The modulatory role of the noradrenergic system (2000) FEBS Lett, 487, pp. 171-175
  • Gleason, D.F., Classification of prostatic carcinomas (1966) Cancer Chemother Rep, 50, pp. 125-128
  • Goodman, A.I., Choudhury, M., da Silva, J.L., Schwartzman, M.L., Abraham, N.G., Overexpression of the heme oxygenase gene in renal cell carcinoma (1997) Proc Soc Exp Biol Med, 214, pp. 54-61
  • Guehmann, S., Vorbrueggen, G., Kalkbrenner, F., Moelling, K., Reduction of a conserved Cys is essential for Myb DNA-binding (1992) Nucleic Acids Res, 20, pp. 2279-2286
  • Hill, M., Pereira, V., Chauveau, C., Zagani, R., Remy, S., Tesson, L., Mazal, D., Anegon, I., Heme oxygenase-1 inhibits rat and human breast cancer cell proliferation: Mutual cross inhibition with indoleamine 2,3-dioxygenase (2005) FASEB J, 19, pp. 1957-1968
  • Hon, T., Hach, A., Tamalis, D., Zhu, Y., Zhang, L., The yeast heme-responsive transcriptional activator Hap1 is a preexisting dimer in the absence of heme (1999) J Biol Chem, 274, pp. 22770-22774
  • Huang, L.E., Arany, Z., Livingston, D.M., Bunn, H.F., Activation of hypoxia-inducible transcription factor depends primarily upon redox-sensitive stabilization of its alpha subunit (1996) J Biol Chem, 271, pp. 32253-32259
  • Huang, R.P., Adamson, E.D., Characterization of the DNA-binding properties of the early growth response-1 (Egr-1) transcription factor: Evidence for modulation by a redox mechanism (1993) DNA Cell Biol, 12, pp. 265-273
  • Kikuchi, G., Yoshida, T., Noguchi, M., Heme oxygenase and heme degradation (2005) Biochem Biophys Res Commun, 338, pp. 558-567
  • Klein, E.A., Casey, G., Silverman, R., Genetic susceptibility and oxidative stress in prostate cancer: Integrated model with implications for prevention (2006) Urology, 68, pp. 1145-1151
  • Li Volti, G., Ientile, R., Abraham, N.G., Vanella, A., Cannavo, G., Mazza, F., Curro, M., Campisi, A., Immunocytochemical localization and expression of heme oxygenase-1 in primary astroglial cell cultures during differentiation: Effect of glutamate (2004) Biochem Biophys Res Commun, 315, pp. 517-524
  • Lin, Q., Weis, S., Yang, G., Weng, Y.H., Helston, R., Rish, K., Smith, A., Dennery, P.A., Heme oxygenase-1 protein localizes to the nucleus and activates transcription factors important in oxidative stress (2007) J Biol Chem, 282, pp. 20621-20633
  • Liu, Z.M., Chen, G.G., Ng, E.K., Leung, W.K., Sung, J.J., Chung, S.C., Upregulation of heme oxygenase-1 and p21 confers resistance to apoptosis in human gastric cancer cells (2004) Oncogene, 23, pp. 503-513
  • Lo, S., Di, P.S., Yusuf, H., McCombe, A.W., Constitutive (HO-2) and inducible (HO-1) haem oxygenase in pleomorphic adenomas of the human parotid: An immunocytochemical study (2005) J Laryngol Otol, 119, pp. 179-183
  • Maines, M.D., The heme oxygenase system and its functions in the brain (2000) Cell Mol Biol (Noisy-le-grand), 46, pp. 573-585
  • Maines, M.D., The heme oxygenase system: Update 2005 (2005) Antioxid Redox Signal, 7, pp. 1761-1766
  • Maines, M.D., Abrahamsson, P.A., Expression of heme oxygenase-1 (HSP32) in human prostate: Normal, hyperplastic, and tumor tissue distribution (1996) Urology, 47, pp. 727-733
  • Maines, M.D., Gibbs, P.E., 30 some years of heme oxygenase: From a 'molecular wrecking ball' to a 'mesmerizing' trigger of cellular events (2005) Biochem Biophys Res Commun, 338, pp. 568-577
  • Malins, D.C., Johnson, P.M., Wheeler, T.M., Barker, E.A., Polissar, N.L., Vinson, M.A., Age-related radical-induced DNA damage is linked to prostate cancer (2001) Cancer Res, 61, pp. 6025-6028
  • Mayerhofer, M., Florian, S., Krauth, M.T., Aichberger, K.J., Bilban, M., Marculescu, R., Printz, D., Sillaber, C., Identification of heme oxygenase-1 as a novel BCR/ABL-dependent survival factor in chronic myeloid leukemia (2004) Cancer Res, 64, pp. 3148-3154
  • Morse, D., Choi, A.M., Heme oxygenase-1: From bench to bedside (2005) Am J Respir Crit Care Med, 172, pp. 660-670
  • Nishie, A., Ono, M., Shono, T., Fukushi, J., Otsubo, M., Onoue, H., Ito, Y., Kuwano, M., Macrophage infiltration and heme oxygenase-1 expression correlate with angiogenesis in human gliomas (1999) Clin Cancer Res, 5, pp. 1107-1113
  • Oates, P.S., West, A.R., Heme in intestinal epithelial cell turnover, differentiation, detoxification, inflammation, carcinogenesis, absorption and motility (2006) World J Gastroenterol, 12, pp. 4281-4295
  • Ohshima, H., Tatemichi, M., Sawa, T., Chemical basis of inflammation-induced carcinogenesis (2003) Arch Biochem Biophys, 417, pp. 3-11
  • Parfenova H, Neff III RA, Alonso JS, Shlopov BV, Jamal CN, Sarkisova SA, Leffler CW (2001) Cerebral vascular endothelial heme oxygenase: expression, localization, and activation by glutamate. Am J Physiol Cell Physiol 281: C1954-C1963; Prawan, A., Kundu, J.K., Surh, Y.J., Molecular basis of heme oxygenase-1 induction: Implications for chemoprevention and chemoprotection (2005) Antioxid Redox Signal, 7, pp. 1688-1703
  • Sacca, P., Caballero, F., Batlle, A., Vazquez, E., Cell cycle arrest and modulation of HO-1 expression induced by acetyl salicylic acid in hepatocarcinogenesis (2004) Int J Biochem Cell Biol, 36, pp. 1945-1953
  • Segui-Simarro, J.M., Testillano, P.S., Risueno, M.C., Hsp70 and Hsp90 change their expression and subcellular localization after microspore embryogenesis induction in Brassica napus L (2003) J Struct Biol, 142, pp. 379-391
  • Suttner, D.M., Sridhar, K., Lee, C.S., Tomura, T., Hansen, T.N., Dennery, P.A., Protective effects of transient HO-1 overexpression on susceptibility to oxygen toxicity in lung cells (1999) Am J Physiol, 276, pp. L443-L451
  • Tanaka, S., Akaike, T., Fang, J., Beppu, T., Ogawa, M., Tamura, F., Miyamoto, Y., Maeda, H., Antiapoptotic effect of haem oxygenase-1 induced by nitric oxide in experimental solid tumour (2003) Br J Cancer, 88, pp. 902-909
  • Tenhunen, R., Marver, H.S., Schmid, R., The enzymatic conversion of heme to bilirubin by microsomal heme oxygenase (1968) Proc Natl Acad Sci USA, 61, pp. 748-755
  • Toyokuni, S., Novel aspects of oxidative stress-associated carcinogenesis (2006) Antioxid Redox Signal, 8, pp. 1373-1377
  • Toyokuni, S., Okamoto, K., Yodoi, J., Hiai, H., Persistent oxidative stress in cancer (1995) FEBS Lett, 358, pp. 1-3
  • Tsuji, M.H., Yanagawa, T., Iwasa, S., Tabuchi, K., Onizawa, K., Bannai, S., Toyooka, H., Yoshida, H., Heme oxygenase-1 expression in oral squamous cell carcinoma as involved in lymph node metastasis (1999) Cancer Lett, 138, pp. 53-59
  • Vazquez, E., Gerez, E., Caballero, F., Olivieri, L., Falcoff, N., Tomaro, M.L., Batlle, A., On the promoting action of tamoxifen in the p-dimethylaminoazobenzene induced hepatocarcinogenesis CF1 mice model and the cytoprotective role of heme oxygenase (2002) Heme Oxygenase in Biology and Medicine, pp. 469-479. , Abraham NG, Alan J, Nath K eds, pp, New York, Boston, Dordrecht, London, Moscow: Kluwer Academic/Plenum
  • Wasylyk, C., Wasylyk, B., Oncogenic conversion of Ets affects redox regulation in-vivo and in-vitro (1993) Nucleic Acids Res, 21, pp. 523-529
  • Willis, D., Overview of HO-1 in inflammatory pathologies (1999) Inducible Enzymes in the Inflammatory Response, pp. 55-92. , Willoughby DA, Tomlinson A eds, pp, Basel: Birkauser Verlag
  • Willis, D., Moore, A.R., Frederick, R., Willoughby, D.A., Heme oxygenase: A novel target for the modulation of the inflammatory response (1996) Nat Med, 2, pp. 87-90
  • Yilmaz, M.I., Saglam, K., Sonmez, A., Gok, D.E., Basal, S., Kilic, S., Akay, C., Kocar, I.H., Antioxidant system activation in prostate cancer (2004) Biol Trace Elem Res, 98, pp. 13-19

Citas:

---------- APA ----------
Sacca, P., Meiss, R., Casas, G., Mazza, O., Calvo, J.C., Navone, N. & Vazquez, E. (2007) . Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer. British Journal of Cancer, 97(12), 1683-1689.
http://dx.doi.org/10.1038/sj.bjc.6604081
---------- CHICAGO ----------
Sacca, P., Meiss, R., Casas, G., Mazza, O., Calvo, J.C., Navone, N., et al. "Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer" . British Journal of Cancer 97, no. 12 (2007) : 1683-1689.
http://dx.doi.org/10.1038/sj.bjc.6604081
---------- MLA ----------
Sacca, P., Meiss, R., Casas, G., Mazza, O., Calvo, J.C., Navone, N., et al. "Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer" . British Journal of Cancer, vol. 97, no. 12, 2007, pp. 1683-1689.
http://dx.doi.org/10.1038/sj.bjc.6604081
---------- VANCOUVER ----------
Sacca, P., Meiss, R., Casas, G., Mazza, O., Calvo, J.C., Navone, N., et al. Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer. Br. J. Cancer. 2007;97(12):1683-1689.
http://dx.doi.org/10.1038/sj.bjc.6604081