Navegar

Colección

Datos Estadísticas

Artículo

Este artículo es de Acceso Abierto y puede ser descargado en su versión final desde nuestro repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

δ-aminolevulinic acid (ALA) is the precursor in the biosynthesis of porphyrins. The knowledge of both the regulation of ALA entrance and efflux from the cells and the control of porphyrin biosynthesis is essential to improve ALA-mediated photodynamic therapy. In this work, we studied the regulation of ALA uptake and efflux by endogenously accumulated ALA and/or porphyrins in murine mammary adenocarcinoma cells. Under our set of conditions, the haem synthesis inhibitor succinyl acetone completely prevented porphobilinogen and porphyrin synthesis from ALA, and led to an increase in the intracellular ALA pool. However, neither intracellular ALA nor porphyrin pools regulate ALA uptake or efflux during the first 15 min of the process. Based on temperature dependence data, ALA but not γ-aminobutyric acid (GABA) efflux is mediated by a diffusion mechanism. Moreover, the addition of extracellular GABA not only did not influence the rate of ALA efflux but on the contrary it affected ALA uptake, showing the contribution of a saturable mechanism for the uptake, but not for the efflux of ALA from the cells. © 2003 Cancer Research UK.

Registro:

Documento: Artículo
Título:Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
Autor:Correa García, S.; Casas, A.; Perotti, C.; Batlle, A.; Bermúdez Moretti, M.
Filiación:Ctro. Invest. Porfirinas y Porfirias, FCEN, University of Buenos Aires, Buenos Aires, Argentina
CONICET, Ciudad Universitaria, Pabellón II, 2 Piso, Buenos Aires, 1428, Argentina
Viamonte 1881, 10A, C1056ABA, Buenos Aires, Argentina
Palabras clave:δ-aminolevulinic acid; ALA efflux; ALA uptake; Photodynamic therapy; 4 aminobutyric acid; aminolevulinic acid; heme derivative; porphobilinogen; porphyrin; succinylacetone; animal cell; article; breast adenocarcinoma; cancer cell culture; cell transport; controlled study; diffusion; heme synthesis; mouse; nonhuman; photodynamic therapy; priority journal; protein function; protein protein interaction; protein transport; temperature dependence; Adenocarcinoma; Aminolevulinic Acid; Animals; gamma-Aminobutyric Acid; Mammary Neoplasms, Animal; Mice; Photochemotherapy; Photosensitizing Agents; Porphyrins; Tumor Cells, Cultured
Año:2003
Volumen:89
Número:1
Página de inicio:173
Página de fin:177
DOI: http://dx.doi.org/10.1038/sj.bjc.6601066
Título revista:British Journal of Cancer
Título revista abreviado:Br. J. Cancer
ISSN:00070920
CODEN:BJCAA
CAS:4 aminobutyric acid, 28805-76-7, 56-12-2; aminolevulinic acid, 106-60-5; porphobilinogen, 487-90-1; porphyrin, 24869-67-8; succinylacetone, 51568-18-4; Aminolevulinic Acid, 106-60-5; gamma-Aminobutyric Acid, 56-12-2; Photosensitizing Agents; Porphyrins
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00070920_v89_n1_p173_CorreaGarcia.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v89_n1_p173_CorreaGarcia

Referencias:

  • Bermúdez Moretti, M., Correa García, S., Perotti, C., Batlle, A., Casas, A., δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by BETA transporters (2002) Br J Cancer, 87, pp. 471-474
  • Döring, F., Walter, J., Will, J., Föcking, M., Boll, M., Amasheh, S., Clauss, W., Daniel, H., Delta-aminolevulinic acid transport by intestinal and renal peptide transporters and its physiological and clinical implications (1998) J Clin Invest, 101, pp. 2761-2767
  • Dougherty, T., Kaufman, J., Goldfarb, A., Weishaupt, K., Boyle, D., Mittleman, A., Photoradiation therapy for the treatment of malignant tumors (1978) Cancer Res, 38, pp. 2638-2635
  • Ebert, P., Hess, R., Frykholm, B., Tschudy, D., Succinylacetone, a potent inhibitor of heme biosynthesis: Effect on cell growth, heme content and delta-aminolevulinic acid dehydratase activity of malignant murine erythroleukemia cells (1979) Biochem Biophys Res Commun, 88, pp. 1382-1390
  • Fukuda, H., Casas, A., Chueke, F., Paredes, S., Batlle, A., Photodynamic action of endogenously synthesized porphyrins from aminolevulinic acid, using a new model for assaying the effectiveness of tumoral cell killing (1993) Int J Biochem, 25, pp. 1395-1398
  • Fukuda, H., Batlle, A., Riley, P., Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA (1993) Int J Biochem, 25, pp. 1407-1410
  • Gibson, S., Cuprkis, D., Havens, J., Nguyen, M., Hilf, R., A regulatory role for porphobilinogen deaminase (PBGD) in 5-aminolevulinic acid (5-ALA)-induced photosensitization? (1998) Br J Cancer, 77, pp. 235-243
  • Gibson, S., Havens, J., Metz, L., Hilf, R., Is δ-aminolevulinic acid dehydratase rate limiting in heme biosynthesis following exposure of cells to aminolevulinic acid? (2001) Photochem Photobiol, 73, pp. 312-317
  • Iinuma, S., Farshi, S., Ortel, B., Hasan, H., A mechanistic study of cellular photodestruction with 5-aminolevulinic acid-induced porphyrin (1994) Br J Cancer, 70, pp. 21-28
  • Kennedy, J., Pottier, R., Pross, G., Photodynamic therapy with endogenous protoporphyrin IX: Basic principles and present clinical experience (1990) J Photochem Photobiol B, 6, pp. 143-148
  • Kriegmair, M., Baumgartner, R., Knuechel, R., Stepp, H., Hofstadter, F., Hofstetter, A., Detection of early bladder cancer by 5-aminolevulinic acid induced porphyrin fluorescence (1996) J Urol, 155, pp. 105-110
  • Malik, Z., Djaldetti, M., 5-Aminolevulinic acid stimulation of porphyrin and hemoglobin synthesis by uninduced Friend erythroleukemic cells (1979) Cell Differ, 8, pp. 223-233
  • Mauzerall, M., Granick, S., The occurrence and determination of 5-aminolevulinic acid and porphobilinogen in urine (1956) J Biol Chem, 219, pp. 435-437
  • Navone, N., Frisardi Resnik, E., Batlle, A., Polo, C., Porphyrin biosynthesis in human breast cancer. Preliminary mimetic in vitro studies (1988) Med Sci Res, 16, pp. 61-62
  • Navone, N., Polo, C., Frisardi, A., Batlle, A., Mouse mammary carcinoma PBGase and hydroxymethylbilane synthetase (1991) Comp Biochem Physiol B, 98, pp. 67-71
  • Novotny, A., Xiang, J., Stummer, W., Teuscher, N., Smith, D., Keep, R., Mechanisms of 5-aminolevulinic acid uptake at the choroid plexus (2000) J Neurochem, 75, pp. 321-328
  • Palacín, M., Estévez, R., Bertran, J., Zorzano, A., Molecular biology of mammalian plasma membrane amino acids transporters (1998) Physiol Rev, 78, pp. 969-1054
  • Pottier, R., Chow, Y., La Plante, J.-P., Truscott, T., Kennedy, J., Beiner, L., Non-invasive technique for obtaining fluorescence excitation and emission spectra in vivo (1986) Photochem Photobiol, 44, pp. 679-687
  • Rimington, C., Porphyrin and haeme biosynthesis and its control (1966) Acta Med Scand, 176, pp. 11-24
  • Rud, E., Gederaas, O., Hogset, A., Berg, K., 5-Aminolevulinic acid, but not 5-aminolevulinic acid esters, is transported into adenocarcinoma cells by system BETA transporters (2000) Photochem Photobiol, 73, pp. 164-169
  • Sinna, A., Kennedy, J., Balkeslee, D., Robertson, D., Experimental porphyric neuropathy: A preliminary report (1981) Can J Neurol Sci, 8, pp. 105-114
  • Werbajh, S.E., Urtreger, A.J., Puricelli, L.I., De Lustig, E.S., Bal De KierJoffe, E., Kornblihtt, A.R., Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells (1998) FEBS Lett, 440, pp. 277-281
  • Whitaker, C., Battah, S., Forsyth, M., Edwards, C., Boyle, R., Matthews, K., Photosensitization of pancreatic tumour cells by 5-aminolevulinic acid esters (2000) Anti-Cancer Drugs Desi, 15, pp. 161-170

Citas:

---------- APA ----------
Correa García, S., Casas, A., Perotti, C., Batlle, A. & Bermúdez Moretti, M. (2003) . Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line. British Journal of Cancer, 89(1), 173-177.
http://dx.doi.org/10.1038/sj.bjc.6601066
---------- CHICAGO ----------
Correa García, S., Casas, A., Perotti, C., Batlle, A., Bermúdez Moretti, M. "Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line" . British Journal of Cancer 89, no. 1 (2003) : 173-177.
http://dx.doi.org/10.1038/sj.bjc.6601066
---------- MLA ----------
Correa García, S., Casas, A., Perotti, C., Batlle, A., Bermúdez Moretti, M. "Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line" . British Journal of Cancer, vol. 89, no. 1, 2003, pp. 173-177.
http://dx.doi.org/10.1038/sj.bjc.6601066
---------- VANCOUVER ----------
Correa García, S., Casas, A., Perotti, C., Batlle, A., Bermúdez Moretti, M. Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line. Br. J. Cancer. 2003;89(1):173-177.
http://dx.doi.org/10.1038/sj.bjc.6601066