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δ-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the δ-aminolevulinic acid transport system in the murine mammary adenocarcinoma cell line LM3 using 14C-δ-aminolevulinic acid, to finally improve δ-aminolevulinic acid incorporation in mammalian cells. Our results showed that δ aminolevulinic acid is incorporated into these cells by two different mechanisms, passive diffusion which is important at the beginning of the incubation, and active transport. Specificity assays suggested that the transporter involved in δ-aminolevulinic acid incorporation is a BETA transporter, probably GAT-2. © 2002 Cancer Research UK.


Documento: Artículo
Título:δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
Autor:Bermúdez Moretti, M.; Correa García, S.; Perotti, C.; Batlle, A.; Casas, A.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), FCEN, University of Buenos Aires, CONICET. Ciudad Universitaria, Pabellón II, 2° Piso, 1428, Buenos Aires, Argentina
Palabras clave:BETA transporters; Membrane transport; Photodynamic therapy; δ-aminolevulinic acid; aminolevulinic acid; carrier protein; animal cell; animal model; article; breast adenocarcinoma; cancer cell culture; cell membrane transport; controlled study; diffusion; drug transport; drug uptake; mouse; nonhuman; priority journal; transport kinetics; Adenocarcinoma; Aminolevulinic Acid; Animals; Biological Transport, Active; Mammary Neoplasms, Experimental; Mice; Tumor Cells, Cultured
Página de inicio:471
Página de fin:474
Título revista:British Journal of Cancer
Título revista abreviado:Br. J. Cancer
CAS:Aminolevulinic Acid, 106-60-5


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---------- APA ----------
Bermúdez Moretti, M., Correa García, S., Perotti, C., Batlle, A. & Casas, A. (2002) . δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters. British Journal of Cancer, 87(4), 471-474.
---------- CHICAGO ----------
Bermúdez Moretti, M., Correa García, S., Perotti, C., Batlle, A., Casas, A. "δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters" . British Journal of Cancer 87, no. 4 (2002) : 471-474.
---------- MLA ----------
Bermúdez Moretti, M., Correa García, S., Perotti, C., Batlle, A., Casas, A. "δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters" . British Journal of Cancer, vol. 87, no. 4, 2002, pp. 471-474.
---------- VANCOUVER ----------
Bermúdez Moretti, M., Correa García, S., Perotti, C., Batlle, A., Casas, A. δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters. Br. J. Cancer. 2002;87(4):471-474.