Artículo
Casas, A.; Perotti, C.; Saccoliti, M.; Sacca, P.; Fukuda, H.; Batlle, A.M.D.C. "ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures" (2002) British Journal of Cancer. 86(5):837-842
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Abstract:
In spite of the wide range of tumours successfully treated with 5-aminolevulinic acid mediated photodynamic therapy, the fact that 5-aminolevulinic acid has low lipid solubility, limits its clinical application. More lipophilic 5-aminolevulinic acid prodrugs and the use of liposomal carriers are two approaches aimed at improving 5-aminolevulinic acid transmembrane access. In this study we used both 5-aminolevulinic acid and its hexyl ester in their free and encapsulated formulations to compare their corresponding endogenous synthesis of porphyrins. Employing murine tumour cultures, we found that neither the use of hexyl ester nor the entrappment of either 5-aminolevulinic acid or hexyl ester into liposomes increase the rate of tumour porphyrin synthesis. By light and electronic microscopy it was demonstrated that exposure of tumour explants to either free or liposomal 5-aminolevulinic acid and subsequent illumination induces the same type of subcellullar damage. Mitochondria, endoplasmic reticulum and plasma membrane are the structures mostly injured in the early steps of photodynamic treatment. In a later stage, cytoplasmic and nuclear disintegration are observed. By electronic microscopy the involvement of the endocytic pathway in the incorporation of liposomal 5-aminolevulinic acid into the cells was shown. © 2002 Cancer Research UK.
Registro:
| Documento: | Artículo |
| Título: | ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures |
| Autor: | Casas, A.; Perotti, C.; Saccoliti, M.; Sacca, P.; Fukuda, H.; Batlle, A.M.D.C. |
| Filiación: | Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP) FCEyN (University of Buenos Aires and CONICET), Ciudad Universitaria, Pabellón II, 2do piso; (1428), Buenos Aires, Argentina Servicio de Patología, Hospital Durand, Buenos Aires, Argentina |
| Palabras clave: | ALA; ALA derivatives; Aminolevulinic acid; Hexyl-ALA; Liposomes; PDT; Photodynamic therapy; aminolevulinic acid; liposome; porphyrin; prodrug; animal cell; animal experiment; animal model; animal tissue; article; cell damage; cell membrane; controlled study; drug formulation; encapsulation; endocytosis; endoplasmic reticulum; laser; lipophilicity; male; mouse; nonhuman; organ culture; phospholipid vesicle; photodynamic therapy; photosensitivity; priority journal; protein localization; protein structure; tumor cell culture; Aminolevulinic Acid; Animals; Liposomes; Male; Mice; Mice, Inbred BALB C; Microscopy, Electron; Mitochondria; Photochemotherapy; Photosensitizing Agents; Porphyrins; Tumor Cells, Cultured |
| Año: | 2002 |
| Volumen: | 86 |
| Número: | 5 |
| Página de inicio: | 837 |
| Página de fin: | 842 |
| DOI: | http://dx.doi.org/10.1038/sj.bjc.6600144 |
| Título revista: | British Journal of Cancer |
| Título revista abreviado: | Br. J. Cancer |
| ISSN: | 00070920 |
| CAS: | 5-aminolevulinic acid hexyl ester; Aminolevulinic Acid, 106-60-5; Liposomes; Photosensitizing Agents; Porphyrins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v86_n5_p837_Casas |
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Citas:
---------- APA ----------
Casas, A., Perotti, C., Saccoliti, M., Sacca, P., Fukuda, H. & Batlle, A.M.D.C. (2002) . ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures. British Journal of Cancer, 86(5), 837-842.http://dx.doi.org/10.1038/sj.bjc.6600144
---------- CHICAGO ----------
Casas, A., Perotti, C., Saccoliti, M., Sacca, P., Fukuda, H., Batlle, A.M.D.C. "ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures" . British Journal of Cancer 86, no. 5 (2002) : 837-842.http://dx.doi.org/10.1038/sj.bjc.6600144
---------- MLA ----------
Casas, A., Perotti, C., Saccoliti, M., Sacca, P., Fukuda, H., Batlle, A.M.D.C. "ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures" . British Journal of Cancer, vol. 86, no. 5, 2002, pp. 837-842.http://dx.doi.org/10.1038/sj.bjc.6600144
---------- VANCOUVER ----------
Casas, A., Perotti, C., Saccoliti, M., Sacca, P., Fukuda, H., Batlle, A.M.D.C. ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures. Br. J. Cancer. 2002;86(5):837-842.http://dx.doi.org/10.1038/sj.bjc.6600144
