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Abstract:

The induction of gene expression has been correlated with long-lasting neuronal plasticity and long-term memory (LTM) formation. The fast activation of constitutive transcription factors by signaling mechanisms is thought to be the link between synaptic events and gene expression. However, only one constitutive transcription factor, CREB, has been shown to play a key role in several memory paradigms, both in vertebrates and invertebrates. Here, we report evidences for Rel/NFκ-B constitutive transcription factors participation in memory. Using the LTM paradigm in the crab Chasmagnathus, an enhancement of NFκ-B DNA-binding activity was found after spaced training, which induces LTM, but not after massed training which yields an intermediate-term memory (ITM). Such finding is correlated with the requirement of protein synthesis for LTM consolidation but not for ITM. Furthermore, NFκ-B activation was observed after 15 or 30 training trials, which are sufficient to induce LTM, but not after 5 or 10 trials, a number of trials insufficient to induce LTM. The kinetics of activation was studied and two waves of DNA-binding activity were found, similar to the time course described in other systems. NFκ-B activation after training was also found in synaptosomal extracts. The latter result supports the hypothesis of a novel synapse-to-nucleus signaling system, in which the transcription factor is locally activated by synaptic events and then transported to the nucleus. Copyright (C) 2000 Elsevier Science B.V.

Registro:

Documento: Artículo
Título:Participation of Rel/NF-κB transcription factors in long-term memory in the crab Chasmagnathus
Autor:Freudenthal, R.; Romano, A.
Filiación:Departamento de Ciencias Biologicas, Universidad de Buenos Aires, Ciudad Universitaria, Pab II (1428), Buenos Aires, Argentina
Palabras clave:Crab; EMSA; Long-term memory; Rel/NFκ-B/Dorsal; Transcription factor; immunoglobulin enhancer binding protein; transcription factor; animal experiment; animal tissue; article; controlled study; crab; DNA binding; hypothesis; long term memory; male; memory; nonhuman; priority journal; training; Animals; Base Sequence; Brachyura; Conditioning (Psychology); Male; Memory; NF-kappa B; Oligodeoxyribonucleotides; Proto-Oncogene Proteins c-rel; Signal Transduction; Substrate Specificity; Time Factors; Transcription Factors
Año:2000
Volumen:855
Número:2
Página de inicio:274
Página de fin:281
DOI: http://dx.doi.org/10.1016/S0006-8993(99)02358-6
Título revista:Brain Research
Título revista abreviado:Brain Res.
ISSN:00068993
CODEN:BRREA
CAS:NF-kappa B; Oligodeoxyribonucleotides; Proto-Oncogene Proteins c-rel; Transcription Factors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00068993_v855_n2_p274_Freudenthal

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Citas:

---------- APA ----------
Freudenthal, R. & Romano, A. (2000) . Participation of Rel/NF-κB transcription factors in long-term memory in the crab Chasmagnathus. Brain Research, 855(2), 274-281.
http://dx.doi.org/10.1016/S0006-8993(99)02358-6
---------- CHICAGO ----------
Freudenthal, R., Romano, A. "Participation of Rel/NF-κB transcription factors in long-term memory in the crab Chasmagnathus" . Brain Research 855, no. 2 (2000) : 274-281.
http://dx.doi.org/10.1016/S0006-8993(99)02358-6
---------- MLA ----------
Freudenthal, R., Romano, A. "Participation of Rel/NF-κB transcription factors in long-term memory in the crab Chasmagnathus" . Brain Research, vol. 855, no. 2, 2000, pp. 274-281.
http://dx.doi.org/10.1016/S0006-8993(99)02358-6
---------- VANCOUVER ----------
Freudenthal, R., Romano, A. Participation of Rel/NF-κB transcription factors in long-term memory in the crab Chasmagnathus. Brain Res. 2000;855(2):274-281.
http://dx.doi.org/10.1016/S0006-8993(99)02358-6